12 results about "Oscillarin" patented technology

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-10 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.21-5.70 times.

The present application belongs to the technical field of oscillator, especially a constant-temperature oscillator for vaccine shake flask culture, aiming at the problem that the existing constant-temperature oscillator cannot clamp and fix the shake flask, and the corresponding water bath constant-temperature oscillator and air bath constant-temperature oscillator need to be used, thereby greatly increasing the culture cost, the present application proposes the following scheme, including a box body, a shaking plate is arranged in the box body, a plurality of clamping members for clamping the shake flask are arranged on the top of the shaking plate, U-shaped handles are fixed on the two sides of the top of the shaking plate, the shaking plate is lifted by the U-shaped handles, a temperature sensor is fixed on the inner wall of the bottom of the box body, a water tank and a heating tank are further included, the water tank is fixed on the bottom of the box body, the present application can perform air bath and water bath on the shake flask on the same equipment, the water bath and the air bath can be freely switched by the switching structure, the operation is simple, the cost of vaccine shake flask culture is greatly reduced, and the shake flask can be clamped in all directions by the cooperation of the clamping members, the first spring and the pressing plate during shaking.

The utility model relates to the technical field of bacillus subtilis production, and discloses a constant-temperature oscillator for bacillus subtilis production, which comprises an oscillation box body, a stainless steel sealing cover is arranged at the top of the oscillation box body, a control panel is fixedly connected to the right end of the oscillation box body, a processing chamber is arranged in the oscillation box body, and the processing chamber is communicated with the stainless steel sealing cover. A processing mechanism is arranged in the oscillation box body, a constant temperature control mechanism is arranged on the surface of the oscillation box body, the processing mechanism comprises an oscillation assembly and a device placing assembly, after the device is started, a motor is controlled to drive a rotating shaft and a half gear to rotate, and a placing box is driven to move through meshing of the half gear and a rack; and when the rack of the half gear does not make contact with the rack, the placing box rebounds to achieve oscillation, at the moment, telescopic columns and springs arranged on the two sides of the placing box can reduce excessive oscillation amplitude, it is guaranteed that the oscillation amplitude of the placing box is always appropriate, the danger caused by falling of beakers in the placing box is avoided, and the machining efficiency of the device is improved.

1. The name of the design product: the waist line of the oscillator. 2. The use of the design product: the whole product is an oscillator, which is used for the oscillation culture of various liquid and solid compounds such as biology, biochemistry, cells and strains; the part to be protected is the waist line of the oscillator, which is used for supporting the products to be oscillated and cultured. 3. The design points of the design product: the shape of the part to be protected. 4. The picture or photo that best indicates the design points: perspective view. 5. Other circumstances that need to be explained: the area of the dashed line is not the part to be protected, and the solid line part is the part to be protected.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-12 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.69-6.90 times.

A preparation method of amoxicillin micro-powder belongs to the field of preparation of amoxicillin micro-powder, and comprises the following steps: step 1, adding amoxicillin dissolved clear liquid and ammonia water into a first ultrasonic mixer in a hedging manner; 2, when the liquid level in the first ultrasonic mixer reaches the specified height, mixed liquid in the first ultrasonic mixer is fed into a second mixer; 3, the mixed liquid in the first ultrasonic mixer is injected into a second mixer and collides with obstacles in the second mixer; 4, after all mixed liquid in the first ultrasonic mixer in the second mixer is injected, ammonia water is added again for mixing, and crystallization is continued; and step 5, filtering after the crystallization is completed, and drying the solid to obtain the amoxicillin micro powder. By introducing the ultrasonic oscillator, the mixing efficiency of amoxicillin-dissolved clear liquid and ammonia water can be effectively improved, meanwhile, the crystallization liquid in the first mixer is subjected to ultrasonic crushing, and crystals can be further crushed after the crystallization liquid entering the second mixer along with the mixed liquid in the second mixer collides with a barrier at a high speed.

The invention discloses a natural high-stability anthocyanin toner and a preparation method thereof. The natural high-stability anthocyanin toner is prepared from 5-20 g of anthocyanin extract, 0.2-0.8 g of lycium barbarum polysaccharide and 0.02-0.10 g of calcium ions, and the pH is 4-7. The method comprises the following steps: pulping, filtering and centrifuging anthocyanin-rich fruit and vegetable defective products to obtain clear juice, diluting with water, purifying with AB-8 macroporous resin, and freeze-drying to obtain the anthocyanin extract. The preparation method comprises the following steps: dissolving the anthocyanin extract, lycium barbarum polysaccharide and metal ions in deionized water in proportion, adjusting the pH value, reacting in a constant-temperature water bath oscillator, precipitating with absolute ethyl alcohol, centrifuging, dialyzing, filtering, and freeze-drying to obtain the natural high-stability anthocyanin toner. The toner disclosed by the invention remarkably improves the thermal stability of anthocyanin through a composite coordination effect, has excellent color retention rate and degradation resistance under a high-temperature condition, and can be widely applied to the fields of foods, medicines and the like. The preparation technology is simple, the product stability is good, and the important application value is achieved.

The present invention relates to the technical field of continuous flow reactions, and discloses a multi-point oscillation mixing apparatus for chemical reagent production and a production process thereof, the multi-point oscillation mixing apparatus comprises: a reaction tube for continuous passing and mixing of chemical reagents; the plurality of side pipes are arranged along the reaction pipe; the oscillator is arranged in the side pipe and is used for blocking the chemical reagent on the inner side of the side pipe; the side pipes are connected with the piston cylinder through transmission pipelines, and the transmission pipelines are filled with transmission fluid; the main piston reciprocates in the piston cylinder to enable the oscillators in the side pipes to oscillate through the transmission fluid; the driver drives the main piston to reciprocate; by utilizing the reciprocating motion of the main piston in the piston cylinder, the reciprocating motion can be transmitted to the plurality of oscillators through the transmission pipeline and the transmission liquid, so that the multi-point oscillation mixing of chemical reagents in the reaction tube is promoted; the oscillators directly act on chemical reagents in multiple positions of the reaction tube, so that the oscillation mixing effect of the chemical reagents is remarkably improved, and the mixing reaction quality is ensured.

The preparation method of the pressing type access temperature / pH dual-responsive antioxidant and antibacterial hydrogel comprises the following steps: A, synthesis of MY NPs: dissolving DMY in ethanol for ultrasonic dissolution, then adding ultrapure water and hydrochloric acid, adding a formaldehyde solution with the mass concentration of 38% into a DMY dispersion liquid under vigorous stirring, then adding a lysine solution, reacting for 10 minutes, centrifuging and collecting nano-particles, and drying the nano-particles to obtain the MY NPs; b, synthesizing DMY NPs (at) Ag-NPs: mixing a DMY NPs solution with silver nitrate, then vigorously stirring at room temperature until the solution becomes brown yellow, and centrifugally collecting after 4 hours; and C, hydrogel synthesis: mixing F127 with carboxymethyl chitosan, then adding DMY NPs (at) Ag-NPs and water, uniformly mixing on an oscillator, and finally placing the mixture in a constant-temperature water bath at 37 DEG C to obtain the hydrogel. The garment is convenient to use.

The present application relates to the technical field of continuous flow reaction, and discloses a multi-point oscillation mixing device for chemical reagent production and a production process thereof, comprising: a reaction tube for continuously passing through mixing of chemical reagents; a plurality of side tubes arranged along the reaction tube; an oscillator arranged in the side tube and blocking the chemical reagents inside; a piston cylinder, the side tubes are connected with the piston cylinder through transmission pipelines, the transmission pipelines are filled with transmission liquid; a main piston reciprocatingly moving in the piston cylinder to make the oscillator in each side tube oscillate through the transmission liquid; and a driver driving the main piston to reciprocate. The present application utilizes the reciprocating movement of the main piston in the piston cylinder to transmit the reciprocating action to the multiple oscillators through the transmission pipelines and the transmission liquid, promotes the multi-point oscillation mixing of the chemical reagents in the reaction tube, and significantly improves the oscillation mixing effect of the chemical reagents to ensure the mixing reaction quality.