51 results about "Oscillarin" patented technology

The invention relates to an analyzing method for detecting activity of soil xylanase, which comprises the following steps: firstly, weighting n sieved air dry soil samples into n thick test tubes, adding acetic acid buffer solution into each test tube, oscillating by a vortex oscillator, getting soil suspension into 96 micropore plates under the oscillation condition, adding 4-MUB-7-Beta-D-xyloside substrate solution into n-1 holes, and adding water with equal quantity into the other one hole so as to be used as non-substrate contrast, adding substrate solution with equal quantity and water with equal quantity into the (n+1)th hole so as to be used as soli-free contrast, oscillating and culturing under constant temperature; secondly, adding NaOH into the micropore plates to terminate the reaction after the culture is finished; thirdly, performing the fluorimetric determination to resultant of reaction by a multifunctional microplate reader; and fourthly, calculating the activity of the xylanase. Compared with the traditional method, the invention shortens the culture time, omits the operation procedures of filtration, and the like, and simplifies the operation steps; meanwhile, the determination data of fluorescent materials in the micropore plate can be obtained within 15s through the multifunctional microplate reader, huge samples are allowed to be simultaneously determined; and moreover, the invention has high accuracy and easy operation, stable and reliable result and good reproduction quality.

The invention discloses a brasenia schreberi polysaccharide extraction method. According to the method, freeze-dried brasenia schreberi is pulverized, petroleum ether is adopted for extraction degreasing at 50 DEG C, after solvents are dried through volatilization at the room temperature, the crushed brasenia schreberi is placed in a drying box to be dried at 40 DEG C, the dried brasenia schreberi is obtained, a NaOH solution is added, oscillation extraction is carried out on a water bath oscillator, then, the centrifugal treatment is carried out, supernatant containing brasenia schreberi polysaccharide is obtained, the supernatant is extracted out, brasenia schreberi powder precipitates are left, the NaOH solution is added for continuous extraction, the number of extraction times is totally four, the supernatant obtained in each time is merged, the final supernatant containing polysaccharidec is obtained, and the polysaccharidec yield is measured. The brasenia schreberi polysaccharide extraction method has the beneficial effects that the utilization rate of brasenia schreberi raw materials is high, the effect is good, and the yield of brasenia schreberi polysaccharide extracted by the method provided by the invention is high.

A process for preparing the andrographolide compound (or medicine with improved solubility includes such steps as proportionally mixing the andrographolide with solubilizer (cyclodextrin, amino acid, phosphatide, water-soluble high polymer and vegetable oil), adding water or organic cosolvent, mechanical grinding, ultrasonic processing, and mixing with medicinal auxiliary.

The invention discloses a kiwi fruit seed oil liposome oral liquid and a preparation method thereof. The method comprises the following steps: (1) adding a phosphate buffer solution with pH of 6.8 in a constant temperature oscillator, heating in a water bath to 60 DEG C and keeping constant temperature; (2) based on anhydrous ethanol as a solvent, preparing a mixed solution containing15-25mg/ml of lecithin, 5-20mg/ml of cholesterol and 5-20mg/ml of kiwi fruit seed oil; and (3) injecting the mixed solution in the step (2) into the phosphate buffer solution in the step (1), placing in the constant-temperature water bath at the temperature of 60 DEG C, then performing ultrasonic treatment, adding distilled water to a certain volume, sealing and sterilizing to obtain the kiwi fruit seed oil liposome oral liquid. According to the kiwi fruit seed oil liposome oral liquid prepared by the method disclosed by the invention, kiwi fruit seed oil has high stability and safety, unsaturated fatty acid in the kiwi fruit seed oil can be prevented from being oxidized, and the purposes of convenience in transportation and long-term preservation can be further achieved.

The invention relates to a method for detecting in-vitro enzymolysis of cross-linked hyaluronic acid by utilizing water-phase gel permeation chromatography. Whether the cross-linked hyaluronic acid completely achieves enzymolysis is determined by detecting changes of molecular weight of the hyaluronic acid after the enzymolysis. The method comprises putting small granulated cross-linked hyaluronic acid products of 1mL into a color comparison tube, adding 300unis of hyaluronidase, diluting to the volume of one time with water into a water bath chader with the constant temperature of 37 DEG C, starting to time after dilution, fetching 50mum L supernate through a micro syringe every twenty minutes from the twentieth minutes, putting the fetched supernate into a refrigerator and quickly cooling the supernate to the temperature of 5 DEG C, fetching enzymolysis supernate for 3-5 hours, detecting the molecular weight of the supernate at different time intervals respectively through the water-phase gel permeation chromatography (GPC), and determining that the enzymolysis of the product is finished when the molecular weight trends to be a constant. The method for detecting the in-vitro enzymolysis of the cross-linked hyaluronic acid by utilizing the water-phase gel permeation chromatography is simple, easy to operate, low in cost, safe and reliable.

The invention provides a pretreatment method for detection of acetamiprid in dry fruits and vegetable. The method comprises the operations of weighting the sample to be detected, soaking, performing rotary evaporation and vortex elution, centrifuging, filtering and the like. The used instrument comprises a constant-temperature oscillator, a reduced-pressure rotary evaporator, a high-speed freezing centrifuge and the like. The sample solution prepared by the pretreatment method can be directly detected by liquid chromatography-mass spectrography. Compared with the traditional method, the pretreatment method has the advantages of being simple and effective in operation, low in cost, good in repeatability and the like, has very strong operability and significant economic benefits, and is potentially to be popularized and applied on a large scale in the industries of environmental protection, commercial examination and entry-exit inspection and quarantine and the like.

The invention relates to a cadmium-lead-arsenic polluted soil composite eluting agent and a preparation method thereof. The cadmium-lead-arsenic polluted soil composite eluting agent comprises a hydroxyethylidene-1, 1-diphosphonic acid solution, an L-lactic acid solution, a pyruvic acid solution and a FeCl3 solution. The volume ratio of the hydroxyethylidene-1, 1-diphosphonic acid to the L-lactic acid to the pyruvic acid to the FeCl3 solution is 6: 2: 1: 1. The preparation method of the cadmium-lead-arsenic polluted soil composite eluting agent comprises the following steps: (1) mixing 10% of a 1-hydroxyethylidene-1, 1-diphosphonic acid solution, 10% of an L-lactic acid solution, 10% of a pyruvic acid solution and a 0.1 mol/L FeCl3 solution according to a volume ratio of 6: 2: 1: 1 to obtain a mixed solution A; and (2) placing the mixed solution A in a reciprocating oscillator for oscillation treatment, sealing, and storing in a dark place. According to the invention, thehydroxyethylidene-1, 1-diphosphonic acid solution, the L-lactic acid solution, the pyruvic acid solution and the FeCl3 solution are compounded, so that not only can the efficient leaching of the soil polluted by the high cadmium, lead and arsenic be realized, but also the problem of secondary pollution to the soil can be avoided.

The invention provides a palladium ion imprinted polymer and a preparation method and application thereof. The method comprises the steps that template palladium ion PdC142- and functional monomers are mixed and dissolved in methyl alcohol, and placed in a thermostatic oscillator for oscillatory reaction to form a template functional monomer complex, and initiator and a cross-linking agent are added and mixed evenly; nitrogen is introduced in the obtained evenly-mixed solution, and a precipitation polymerization method is adopted to obtain the ion imprinted polymer with template ions; the initiator, the cross-linking agent and the unreacted functional monomers are removed; centrifugal treatment is carried out on an obtained sample, supernate is discarded, the obtained polymer is placed ina vacuum drying oven and dried, and the palladium ion imprinted polymer is obtained. The provided palladium ion imprinted polymer has high affinity and significant selectivity for heavy metal palladium, and can effectively separate and recycle the heavy metal contaminated water body containing palladium.

The invention provides a method for measuring the content of glyceryl triacetate. The method is characterized by including the following steps: (1) putting 0.1 mL of glyceryl triacetate sample in a 50 mL of conical flask; (2) adding 10 mL of acetone; (3) oscillating on an oscillator for 10 minutes, and sampling for waiting for testing on a machine; (4) measuring the sample to be measured by a gas chromatographic method, wherein the gas chromatographic condition and the quantitative method are the same as those of YC144-2008. On the condition that the dilution ratio of an original method is almost unchanged, the measuring method provided by the invention is used for optimizing the sample extracting volume, a vessel, a dilution solvent, solvent dosage and the like of the original method. Compared with the prior art, the measuring method has the advantages of simplifying the operation, improving the efficiency, saving the cost and ensuring better stability.

The invention relates to a method for improving activity of enterococcus faecium glutamate decarboxylase by virtue of 732 cation exchange resin, and belongs to the field of bio-technology. According to the method provided by the invention, the 732 cation exchange resin is taken as an enzyme activity accelerant of the enterococcus faecium glutamate decarboxylase; a 732 cation exchange resin-glutamate decarboxylase composite catalysis system is constructed by mixing the 732 cation exchange resin, an L-glutamic acid solution and an enterococcus faecium suspension or glutamate decarboxylase free enzyme liquid at the ratio of 1 to 1 to 1 of the mass of the 732 cation exchange resin to the volume of the L-glutamic acid solution to the volume of the enterococcus faecium suspension or glutamate decarboxylase free enzyme liquid; and the yield of [gamma]-aminobutyric acid can be improved by 25.31-143.23% when it conducts a reaction in a water-bath oscillator at 80r/min and 37-43 DEG C or it conducts a stirring reaction at a low speed for 24-36h. According to the method provided by the invention, the 732 cation exchange resin can obviously improve the activity of the glutamate decarboxylase,and meanwhile, an exchange adsorption effect of the 732 cation exchange resin on [gamma]-aminobutyric acid is a process of purifying the [gamma]-aminobutyric acid, so that a downstream extraction andpurification process is simplified and production cost is reduced; and the method is simple and convenient and is green and environment-friendly.

The invention discloses a method for improving the jelly strength of gelatin, and the method specifically comprises the following steps: uniformly mixing gelatin, a cationic modifier and water, and then putting the mixture into a constant-temperature oscillator for reaction; after the reaction is finished, standing the reaction liquid, sequentially filtering, evaporating and concentrating the obtained gelatin liquid, performing sterilizing, freezing and forming to obtain jelly, and finally cutting the jelly into blocks; and performing drying and crushing to obtain the modified gelatin. According to the method disclosed by the invention, under the action of the cationic modifier, amino groups and carboxyl groups in the gelatin are mutually crosslinked, the molecular weight is increased, and a more stable three-dimensional network structure is formed, so the jelly strength of the gelatin is improved; the biomass material chitosan in the cationic modifier has good antibacterial ability, so that the antibacterial property of the gelatin is improved, and the gelatin is more beneficial to storage.

The invention discloses an automatic benthonic animal releasing oscillator which comprises a releasing container, the releasing container is arranged on a ship body through a container support, a feeding door and a lower discharging opening are formed in the releasing container, the top of a sliding way is located below the corresponding lower discharging opening, the bottom of the sliding way extends to the position above the top of an oscillating plate, the sliding way and the oscillating plate are both in a slope shape, the oscillating plate is arranged on an oscillating plate support through an oscillating spring, the oscillating plate support is arranged on the ship body, and a vibrator is arranged at the bottom of the oscillating plate. The problems that in water ecological environment treatment, since the benthonic animals are thrown mainly through manpower, releasing is uneven, efficiency is low, manpower and material resources are consumed and the like are solved, and the releasing mode of the benthonic animals is changed; and the automatic benthonic animal releasing oscillator is simple in structure, convenient to use and remarkable in effect.

The invention relates to the technical field of bioengineering, and especially relates to a fluorescent quantitative PCR kit for detecting a vancomycin-resistant vanA gene of enterotoxin. SEQ ID NO:1upstream primer F:5'-GCTACGTTTACCTATCCTG-3', SEQ ID NO:2 downstream primer R:5'-CAGCCTGCTCAATTAAGA-3' and SEQ ID NO:3 TaqMan probe sequence P:5'-FAM-TTGCTGTCATATTGTCTTGCCGATTC-BHQ-3' are designed through software. Fluorescent RT-PCR detection is carried out, an RT-PCR reaction liquid, a hot start Taq enzyme and a probe are taken and mixed into a centrifugal tube according to the number n (n = (thenumber of samples to be detected) + 2) of detection samples, the centrifugal tube is oscillated on a vortex oscillator, sub-packaging is carried out according to each tube, each tube is covered witha tube cover for later use, a negative control liquid is added into a sub-packaging tube, and DNA of each sample is taken, and is added into a corresponding reaction tube; and finally, a positive control is taken out and is added into another reaction tube, and all the reaction tubes are labeled and centrifuged, and are taken out of a fluorescent PCR instrument. The kit can rapidly detect the enterococcus vancomycin VanA drug-resistant gene, and provides guidance for drug therapy after super bacterial infection.

The invention discloses bacillus siamensis capable of degrading grease and the application of the bacillus siamensis in grease-containing wastewater. The bacillus siamensis BS1 disclosed by the invention has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2021840 in the China Center for Type Culture Collection). The bacillus siamensis BS1 disclosed by the invention can be used for degrading grease. In a grease culture medium containing soybean oil with the initial concentration of 9.5-10.5 g/L, after being cultured for 48 hours, the grease degradation rate reaches 82.34%, wherein the culture temperature is controlled at 37 DEG C, and the rotating speed of a constant-temperature culture oscillator is 200 rpm. The invention also discloses the application of the bacillus siamensis BS1 in grease-containing wastewater, and the bacillus siamensis BS1 has a relatively good grease degradation capability.