24 results about "Oscillarin" patented technology

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-10 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.21-5.70 times.

The invention discloses a preparation method of an in-situ encapsulated enzyme material based on a metal polyphenol network, and belongs to the field of immobilized enzyme preparation. The preparation method comprises the following steps: firstly, fully mixing glucose oxidase (GOx) and tannic acid (TA), and combining the glucose oxidase (GOx) and the tannic acid (TA) through hydrogen bonds and hydrophobic interaction to form a TA-GOx compound; then adding a FeCl3. 6H2O solution, oscillating by using a vortex oscillator, and forming a metal polyphenol network through a rapid coordination reaction to package the enzyme inside in a lossless manner, so as to realize the construction of an enzyme in-situ packaging system. The preparation method of the metal polyphenol network in-situ encapsulated enzyme material (20s vortex, room temperature and aqueous solution) is simple, overcomes the limitations that the enzyme is easy to fall off, the enzyme structure is irreversibly damaged and the accessibility of enzyme active sites is poor, and breaks through the size limitation of the enzyme. And the polyphenol and the metal salt are low in industrial price, so that the simple and economical enzyme encapsulation technology has a wide application range and industrial application.

1. The name of the product of this design: drug oscillator. 2. Purpose of this design product: drug oscillation. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The present application belongs to the technical field of oscillator, especially a constant-temperature oscillator for vaccine shake flask culture, aiming at the problem that the existing constant-temperature oscillator cannot clamp and fix the shake flask, and the corresponding water bath constant-temperature oscillator and air bath constant-temperature oscillator need to be used, thereby greatly increasing the culture cost, the present application proposes the following scheme, including a box body, a shaking plate is arranged in the box body, a plurality of clamping members for clamping the shake flask are arranged on the top of the shaking plate, U-shaped handles are fixed on the two sides of the top of the shaking plate, the shaking plate is lifted by the U-shaped handles, a temperature sensor is fixed on the inner wall of the bottom of the box body, a water tank and a heating tank are further included, the water tank is fixed on the bottom of the box body, the present application can perform air bath and water bath on the shake flask on the same equipment, the water bath and the air bath can be freely switched by the switching structure, the operation is simple, the cost of vaccine shake flask culture is greatly reduced, and the shake flask can be clamped in all directions by the cooperation of the clamping members, the first spring and the pressing plate during shaking.

The utility model relates to the technical field of bacillus subtilis production, and discloses a constant-temperature oscillator for bacillus subtilis production, which comprises an oscillation box body, a stainless steel sealing cover is arranged at the top of the oscillation box body, a control panel is fixedly connected to the right end of the oscillation box body, a processing chamber is arranged in the oscillation box body, and the processing chamber is communicated with the stainless steel sealing cover. A processing mechanism is arranged in the oscillation box body, a constant temperature control mechanism is arranged on the surface of the oscillation box body, the processing mechanism comprises an oscillation assembly and a device placing assembly, after the device is started, a motor is controlled to drive a rotating shaft and a half gear to rotate, and a placing box is driven to move through meshing of the half gear and a rack; and when the rack of the half gear does not make contact with the rack, the placing box rebounds to achieve oscillation, at the moment, telescopic columns and springs arranged on the two sides of the placing box can reduce excessive oscillation amplitude, it is guaranteed that the oscillation amplitude of the placing box is always appropriate, the danger caused by falling of beakers in the placing box is avoided, and the machining efficiency of the device is improved.

1. The name of the design product: the waist line of the oscillator. 2. The use of the design product: the whole product is an oscillator, which is used for the oscillation culture of various liquid and solid compounds such as biology, biochemistry, cells and strains; the part to be protected is the waist line of the oscillator, which is used for supporting the products to be oscillated and cultured. 3. The design points of the design product: the shape of the part to be protected. 4. The picture or photo that best indicates the design points: perspective view. 5. Other circumstances that need to be explained: the area of the dashed line is not the part to be protected, and the solid line part is the part to be protected.

The utility model relates to the technical field of bacillus subtilis production, and discloses a constant-temperature oscillator, which comprises an oscillation box fixedly placed on a table top; the sealing cover is arranged at the top of the oscillation box; the movable plate is arranged in the oscillation box; the fixing structure comprises sixteen sets of limiting sleeves, and the limiting sleeves are arranged at the top of the movable plate; the heating structure comprises a first electric control box, and the first electric control box is fixedly installed on the outer wall of one side of the oscillation box; the oscillation structure comprises a sliding groove, and the sliding groove is formed in the bottom of the inner side of the oscillation box; and the second electric control box is fixedly installed on the outer wall of one side of the oscillation box, so that the purposes of carrying out test tube placement on culture bacteria and limiting and fixing the culture bacteria are achieved, the placement stability of the culture bacteria is improved, the culture bacteria are prevented from being scattered in the oscillation process, the use flexibility of the device is improved, and the fixing effect of the device is enhanced.

The invention relates to the technical field of medicine oscillation, in particular to a medicine dissolving oscillator suitable for multiple specifications, which comprises an oscillation base and an oscillation mechanism, the oscillation mechanism comprises a motor, a turntable and an oscillator arranged on the turntable, the motor is arranged in the oscillation base, the turntable is arranged on the oscillation base, and the oscillator is arranged on the turntable. An output shaft of the motor is connected with the middle of the rotary disc and drives the rotary disc and the oscillator on the rotary disc to rotate, the oscillator comprises an oscillation vertical rod, an oscillation transverse rod and a medicine bottle clamping piece, the oscillation transverse rod is arranged on the outer side of the oscillation vertical rod, and the medicine bottle clamping piece is arranged at the end of the oscillation transverse rod. The medicine bottle clamping piece comprises an installation block, a driving assembly and a clamping jaw assembly, and the driving assembly and the clamping jaw assembly are installed on the installation block. A plurality of medicine bottles are clamped on each medicine bottle clamping piece, so that the plurality of medicine bottles can be oscillated at one time, and the efficiency is high; each oscillator is arranged to be of an independent structure, the oscillation speed of the oscillator is adjustable, and it is guaranteed that the oscillator can be suitable for medicine of different specifications.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-12 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.69-6.90 times.

A preparation method of amoxicillin micro-powder belongs to the field of preparation of amoxicillin micro-powder, and comprises the following steps: step 1, adding amoxicillin dissolved clear liquid and ammonia water into a first ultrasonic mixer in a hedging manner; 2, when the liquid level in the first ultrasonic mixer reaches the specified height, mixed liquid in the first ultrasonic mixer is fed into a second mixer; 3, the mixed liquid in the first ultrasonic mixer is injected into a second mixer and collides with obstacles in the second mixer; 4, after all mixed liquid in the first ultrasonic mixer in the second mixer is injected, ammonia water is added again for mixing, and crystallization is continued; and step 5, filtering after the crystallization is completed, and drying the solid to obtain the amoxicillin micro powder. By introducing the ultrasonic oscillator, the mixing efficiency of amoxicillin-dissolved clear liquid and ammonia water can be effectively improved, meanwhile, the crystallization liquid in the first mixer is subjected to ultrasonic crushing, and crystals can be further crushed after the crystallization liquid entering the second mixer along with the mixed liquid in the second mixer collides with a barrier at a high speed.

The invention discloses a natural high-stability anthocyanin toner and a preparation method thereof. The natural high-stability anthocyanin toner is prepared from 5-20 g of anthocyanin extract, 0.2-0.8 g of lycium barbarum polysaccharide and 0.02-0.10 g of calcium ions, and the pH is 4-7. The method comprises the following steps: pulping, filtering and centrifuging anthocyanin-rich fruit and vegetable defective products to obtain clear juice, diluting with water, purifying with AB-8 macroporous resin, and freeze-drying to obtain the anthocyanin extract. The preparation method comprises the following steps: dissolving the anthocyanin extract, lycium barbarum polysaccharide and metal ions in deionized water in proportion, adjusting the pH value, reacting in a constant-temperature water bath oscillator, precipitating with absolute ethyl alcohol, centrifuging, dialyzing, filtering, and freeze-drying to obtain the natural high-stability anthocyanin toner. The toner disclosed by the invention remarkably improves the thermal stability of anthocyanin through a composite coordination effect, has excellent color retention rate and degradation resistance under a high-temperature condition, and can be widely applied to the fields of foods, medicines and the like. The preparation technology is simple, the product stability is good, and the important application value is achieved.

The invention discloses a preparation method of a bakuchiol-loaded exosome and application of the bakuchiol-loaded exosome. The method comprises the following steps: S1, mixing supernate of a collected umbilical cord mesenchymal stem cell hUMSC culture supernate sample with Exosome Concentration Solution, oscillating and uniformly mixing through a vortex oscillator, and then standing at 4 DEG C to capture exosome particles; s2, centrifuging, discarding the supernatant, re-suspending the precipitate, centrifuging, reserving the supernatant, namely an exosome particle crude product, transferring to a purification column, and purifying to obtain purified exosome particles; and S3, mixing the exosome particles obtained in S2 with bakuchiol BAK, and carrying out drug loading treatment by using an ultrasonic cell crusher. The drug-loaded exosome can inhibit inflammation, promote collagen and angiogenesis and accelerate wound healing through subcutaneous injection near a wound, the treatment effect of the exosome is maximized, and the drug-loaded exosome has practical value in the field of skin wound healing.

1. The name of the design product: Oscillator. 2. The use of the design product: A drug mixing and dissolving oscillator, used for dissolving and mixing drugs to prepare uniform suspensions, suspensions, dispersions, etc. 3. The design key points of the design product: lie in the overall shape and structure of the product. 4. The picture or photo that best shows the design key points: the perspective view.

The invention belongs to the technical field of oscillators, particularly relates to a constant-temperature oscillator for vaccine shake-flask culture, and aims to solve the problem that the culture cost is greatly increased due to the fact that an existing shake-flask cannot be clamped and fixed and a corresponding water bath constant-temperature oscillator and a corresponding gas bath constant-temperature oscillator need to be used. An oscillation plate is arranged in the box body, a plurality of clamping components used for clamping shake flasks are arranged at the top of the oscillation plate, U-shaped handles are fixed to the two sides of the top of the oscillation plate and used for lifting the oscillation plate, and a temperature sensor is fixed to the inner wall of the bottom of the box body; the water tank is fixed to the bottom of the box body, air bath and water bath can be carried out on the shake flask on the same equipment, free switching of the water bath and the air bath can be completed through the switching structure, operation is easy, the cost of vaccine shake flask culture is greatly reduced, in addition, during oscillation, the water bath and the water bath can be separated, and the vaccine shake flask culture efficiency is improved. The shake flask can be clamped in all directions through cooperation of the clamping component, the first spring and the pressing plate.

The invention provides composite peptide wine prepared on the basis of various peptides and a preparation method of the composite peptide wine, and belongs to the technical field of functional health-care wine. According to the invention, marine fish skin collagen oligopeptide powder, corn oligopeptide powder, wheat oligopeptide powder and oyster peptide powder are used as raw materials, and plant-derived polypeptide and animal-derived polypeptide are compounded and optimally combined; the base liquor is soaked firstly, so that the content of ascorbic acid and polyphenol is increased, and the antioxidant effect of peptides is further synergistically improved; during preparation, thick slurry is prepared through air stirring, and then the thick slurry is subjected to gradient filtration under different pressures so as to reduce the influence of the solubility of the peptide powder to the maximum extent; meanwhile, air is introduced for stirring when the base liquor is mixed, a high-frequency ultrasonic oscillator is adopted for dispersing to form uniform slurry, and clear liquor is obtained through refined filtration. The obtained composite peptide wine is outstanding in anti-oxidation effect, clear in wine body, free of turbidity after long-term storage, and good in health-care effect and market prospect.

A tobacco product oscillation extraction device relates to the technical field of tobacco product pretreatment and comprises an oscillator body and a clamping device used in cooperation with the oscillator body. The clamping device comprises a pair of fixing seats fixed on two sides of the oscillation table, a plurality of horizontal guide rail groups are arranged between the pair of fixing seats in a crossing mode, and each horizontal guide rail group is composed of a pair of parallel horizontal guide rails. The horizontal guide rail set further comprises a plurality of clamping rods, the two ends of each clamping rod are connected with a pair of parallel horizontal guide rails in a sliding mode respectively, and the clamping rods can slide in the length direction of the horizontal guide rails. And a sponge sleeve is sleeved outside the clamping rod. During use, every two clamping rods can clamp a container from the two sides of the container, and the sponge sleeve deforms under pressure and can adapt to containers of various sizes. Meanwhile, a plurality of horizontal guide rails are matched for use, and a plurality of clamping points in the height direction can be provided for the container, so that the clamping device is adaptive to containers in various shapes such as test tubes, conical flasks and dropping funnels, and the clamping device has better universality.

The present invention relates to the technical field of continuous flow reactions, and discloses a multi-point oscillation mixing apparatus for chemical reagent production and a production process thereof, the multi-point oscillation mixing apparatus comprises: a reaction tube for continuous passing and mixing of chemical reagents; the plurality of side pipes are arranged along the reaction pipe; the oscillator is arranged in the side pipe and is used for blocking the chemical reagent on the inner side of the side pipe; the side pipes are connected with the piston cylinder through transmission pipelines, and the transmission pipelines are filled with transmission fluid; the main piston reciprocates in the piston cylinder to enable the oscillators in the side pipes to oscillate through the transmission fluid; the driver drives the main piston to reciprocate; by utilizing the reciprocating motion of the main piston in the piston cylinder, the reciprocating motion can be transmitted to the plurality of oscillators through the transmission pipeline and the transmission liquid, so that the multi-point oscillation mixing of chemical reagents in the reaction tube is promoted; the oscillators directly act on chemical reagents in multiple positions of the reaction tube, so that the oscillation mixing effect of the chemical reagents is remarkably improved, and the mixing reaction quality is ensured.

The preparation method of the pressing type access temperature / pH dual-responsive antioxidant and antibacterial hydrogel comprises the following steps: A, synthesis of MY NPs: dissolving DMY in ethanol for ultrasonic dissolution, then adding ultrapure water and hydrochloric acid, adding a formaldehyde solution with the mass concentration of 38% into a DMY dispersion liquid under vigorous stirring, then adding a lysine solution, reacting for 10 minutes, centrifuging and collecting nano-particles, and drying the nano-particles to obtain the MY NPs; b, synthesizing DMY NPs (at) Ag-NPs: mixing a DMY NPs solution with silver nitrate, then vigorously stirring at room temperature until the solution becomes brown yellow, and centrifugally collecting after 4 hours; and C, hydrogel synthesis: mixing F127 with carboxymethyl chitosan, then adding DMY NPs (at) Ag-NPs and water, uniformly mixing on an oscillator, and finally placing the mixture in a constant-temperature water bath at 37 DEG C to obtain the hydrogel. The garment is convenient to use.

The invention discloses a preparation method for extracting cyperus esculentus oil through water medium-freezing and unfreezing, and relates to the technical field of food processing. Comprising the following steps: (1) removing impurities, cleaning cyperus esculentus, drying the cyperus esculentus to constant weight at 45 DEG C, crushing the cyperus esculentus, and sieving the crushed cyperus esculentus by (2) weighing 30 g of the cyperus esculentus powder in the step (1), putting the weighed cyperus esculentus powder into a conical flask, adding distilled water according to a material-liquid ratio of 1: 4-1: 7, putting the conical flask into a water bath oscillator, performing oscillation heating to 60 DEG C, taking out the conical flask, and cooling the conical flask to room temperature; (3) adjusting the pH value to 7.00-11.00, incubating for 2-6 hours in a water bath oscillator at 40-80 DEG C, centrifuging for 5 minutes at the speed of 5000r / min, and putting an upper oil layer and an emulsion layer into a 50mL plastic centrifuge tube; and (4) freezing at-5 to-25 DEG C for 8-24 hours, taking out, unfreezing in warm water at 40-80 DEG C for 10-30 minutes, centrifuging at 7000 r / min for 15 minutes, and collecting supernate. According to the invention, the cost is reduced, the oil extraction rate is improved, and the invention provides a green, efficient and economical preparation method of the cyperus esculentus oil.

The invention discloses a preparation method of a sticky rice fragrance extract. The preparation method comprises the following steps: distilling, preparing combined macroporous adsorption resin, adsorbing, eluting and concentrating. Wherein the combined macroporous adsorption resin is prepared by uniformly mixing medium-polarity macroporous adsorption resin and non-polar macroporous adsorption resin according to a weight ratio of 1: (1.2-0.8), then soaking with water, soaking with absolute ethyl alcohol and washing with water. During adsorption, the combined macroporous adsorption resin and the condensate obtained by distillation are mixed, and the mixture is oscillated and adsorbed on a shaking table oscillator; and eluting with an ethanol solution to obtain an eluent, and concentrating to obtain the glutinous rice aroma extract. According to the preparation method of the glutinous rice flavor extract, the macroporous adsorption resin is combined to capture and adsorb the first flavor substance in the condensate, and the obtained glutinous rice flavor extract has natural glutinous rice fragrance and fragrance, and is rich and vivid in fragrance and lasting in fragrance. The invention also provides the glutinous rice fragrance extract and the glutinous rice essence.

The present application relates to the technical field of continuous flow reaction, and discloses a multi-point oscillation mixing device for chemical reagent production and a production process thereof, comprising: a reaction tube for continuously passing through mixing of chemical reagents; a plurality of side tubes arranged along the reaction tube; an oscillator arranged in the side tube and blocking the chemical reagents inside; a piston cylinder, the side tubes are connected with the piston cylinder through transmission pipelines, the transmission pipelines are filled with transmission liquid; a main piston reciprocatingly moving in the piston cylinder to make the oscillator in each side tube oscillate through the transmission liquid; and a driver driving the main piston to reciprocate. The present application utilizes the reciprocating movement of the main piston in the piston cylinder to transmit the reciprocating action to the multiple oscillators through the transmission pipelines and the transmission liquid, promotes the multi-point oscillation mixing of the chemical reagents in the reaction tube, and significantly improves the oscillation mixing effect of the chemical reagents to ensure the mixing reaction quality.