Kiwi fruit seed oil liposome oral liquid and preparation method thereof

An oil liposome, kiwifruit seed technology, applied in the directions of liposome delivery, blood diseases, pharmaceutical formulations, etc., can solve problems such as loss of kiwifruit seed oil, and achieve the effects of promoting commercial value, facilitating long-term storage, and facilitating transportation

Inactive Publication Date: 2012-08-22
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The spray drying method still needs a higher temperature (air inlet 180°C, air outlet 80°C), so there i

Method used

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  • Kiwi fruit seed oil liposome oral liquid and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Determination of Encapsulation Efficiency of Kiwifruit Seed Oil Liposomes

[0036] The content of kiwifruit seed oil was determined by dialysis-ultraviolet spectrophotometry. Pipette 20ml kiwifruit seed oil liposomes into the dialysis bag, after dialysis for 17h, pour the solution in the bag into a 100ml volumetric flask, select ethanol as the demulsifier, combine ultrasound, add sherwood oil to extract, the mixed solution is centrifuged (3000r / min, 3min), take 10ml of supernatant in a 25ml volumetric flask, dilute to volume with petroleum ether, measure its absorbance A at 233nm 1 ; Determination of blank liposome absorbance A with the same method 0 , Calculate △A, calculate the content of encapsulated kiwifruit seed oil from the standard curve.

[0037] Encapsulation rate%=△A*100 / A 1

[0038] 2. Formula and process optimization

[0039] The technical key of liposome oral solution lies in the preparation of liposome. Taking the encapsulation rate as an inde...

Embodiment 2

[0047] Set the constant temperature oscillator temperature to 60 °C and preheat. Accurately measure 100ml of pH 6.8 phosphate buffer solution and place it in a constant temperature shaker, heat it in a water bath to 60°C, and keep it at a constant temperature at a speed of 150r / min. Using absolute ethanol as solvent, respectively prepare lecithin (LC) concentration of 20mg / ml, cholesterol (CH) concentration of 10mg / ml, kiwifruit seed oil (KFO) concentration of 15mg / ml, purity 99.0%. After the solutions were mixed evenly, they were uniformly injected into the phosphate buffer solution in the constant temperature shaker with a syringe. 60°C constant temperature water bath for 30 minutes, remove residual ethanol, ultrasonic treatment for 30 minutes (ultrasonic power 80W), add distilled water to make up to 250ml, seal, irradiate and sterilize to obtain kiwifruit seed oil liposome oral solution.

[0048] The method of the present invention preferably adopts kiwifruit seed oil prov...

Embodiment 3

[0050] Using ethanol as a solvent, respectively prepare lecithin (LC) concentration of 16 mg / ml, cholesterol (CH) concentration of 12 mg / ml, kiwifruit seed oil (KFO) concentration of 10 mg / ml, the preparation method is the same as that of Example 2.

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Abstract

The invention discloses a kiwi fruit seed oil liposome oral liquid and a preparation method thereof. The method comprises the following steps: (1) adding a phosphate buffer solution with pH of 6.8 in a constant temperature oscillator, heating in a water bath to 60 DEG C and keeping constant temperature; (2) based on anhydrous ethanol as a solvent, preparing a mixed solution containing15-25mg/ml of lecithin, 5-20mg/ml of cholesterol and 5-20mg/ml of kiwi fruit seed oil; and (3) injecting the mixed solution in the step (2) into the phosphate buffer solution in the step (1), placing in the constant-temperature water bath at the temperature of 60 DEG C, then performing ultrasonic treatment, adding distilled water to a certain volume, sealing and sterilizing to obtain the kiwi fruit seed oil liposome oral liquid. According to the kiwi fruit seed oil liposome oral liquid prepared by the method disclosed by the invention, kiwi fruit seed oil has high stability and safety, unsaturated fatty acid in the kiwi fruit seed oil can be prevented from being oxidized, and the purposes of convenience in transportation and long-term preservation can be further achieved.

Description

technical field [0001] The invention belongs to the field of health product preparations, and in particular relates to a kiwifruit seed oil liposome oral liquid and a preparation method thereof. Background technique [0002] Kiwi( Actinidia chinensis ) has another name called carambola, vine pear, fairy peach, hair peach, etc. New Zealand kiwifruit is a kind of vine and one of the precious fruits of our country's special products. With the development of kiwi fruit processing industry, the amount of fruit residues has increased. Kiwi fruit residues are mainly fruit crude fiber and seeds. During the processing and production of these factories, a large amount of pomace is produced, which contains a large amount of kiwifruit seed grains. According to Huang Zhuowei et al. (Huang Zhuowei, Yi Minghua. Research on the Extraction of High-quality Edible Oil from Kiwifruit Seeds [J]. Cereals, Oils and Food Science and Technology, 1992, (4): 11~12), the number of seeds in each wild...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K9/127A61P3/06A61P7/02A61P9/12A61P35/00A61P39/06A61K131/00
Inventor 张盛张胜肖文军傅冬和李适刘仲华
Owner HUNAN AGRICULTURAL UNIV
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