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Pretreatment method of residue detection of eight glucocorticoid drugs in chicken

A technology of glucocorticoid and chicken, which is applied in the field of pretreatment of analytical chemistry, can solve the problems of large loss in repeated extraction, long processing time, high economic cost, etc., to save analysis time, save pretreatment cost, and simplify separation steps Effect

Inactive Publication Date: 2015-08-19
FUZHOU UNIV
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Problems solved by technology

The key disadvantages of this type of pretreatment technology are the large loss of repeated extraction, long processing time, high economic cost, and cumbersome process.

Method used

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  • Pretreatment method of residue detection of eight glucocorticoid drugs in chicken
  • Pretreatment method of residue detection of eight glucocorticoid drugs in chicken
  • Pretreatment method of residue detection of eight glucocorticoid drugs in chicken

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Embodiment 1

[0031] The following embodiments describe the specific working principle of the present invention in conjunction with the accompanying drawings:

[0032] The specific operation process of the pretreatment method of the detection of 8 kinds of glucocorticoid drug residues in the said chicken meat of the present invention is as attached figure 1 shown. Including liquid-liquid extraction, rotary evaporation and other operations.

[0033] Specific steps:

[0034] (1) Accurately weigh 2.00 g of the sample, accurate to 0.01 g, put it into a 50 mL centrifuge tube, add 15 mL of ethyl acetate, vortex, centrifuge at 4000 r / min for 15 min, and remove the ethyl acetate layer; Add 10 mL of 0.1 mol / L sodium hydroxide solution, mix well, add 20 mL of ethyl acetate, vortex mix, centrifuge at 4000 r / min for 15 min, remove the ethyl acetate layer; combine the two extracts into a heart bottle Medium, 40 ℃ rotary steamed to nearly dry;

[0035] (2) Add 2 mL of 20% acetonitrile / water and 5 mL ...

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Abstract

The invention provides a pretreatment method of residue detection of eight glucocorticoid drugs in chicken. The method comprises the steps of weighing a to-be-detected sample, carrying out liquid-liquid extraction, carrying out rotary evaporation and the like. The method adopts instruments such as a constant temperature oscillator, a centrifugal machine and the like; the sample solution prepared by the pretreatment method can be directly detected by liquid chromatography-tandem mass spectrometry. Compared with the existing method, the pretreatment method has the advantages of being simple and effective in operation, less in time consumption, low in cost, good in reproducibility and the like; the method has very high operability, can be expected to be popularized and applied in industries such as environmental friendliness, commodity inspection, entry and exit inspection and quarantine and the like on a large scale, and has remarkable economical benefit.

Description

technical field [0001] The invention belongs to the field of pretreatment of analytical chemistry, and more specifically relates to a pretreatment method for detecting eight kinds of glucocorticoids in chicken. Background technique [0002] Glucocorticoids belong to steroid hormones (steroid hormones). Glucocorticoids have a wide range of effects in the body. They are not only necessary for the regulation of sugar, protein, and fat metabolism, but also have the effect of regulating potassium, sodium, and water metabolism. Balance plays an important role. [0003] Glucocorticoids are drugs commonly used in livestock products. They are often used to treat inflammatory reactions, immune diseases, and ketosis in cattle. A common illicit use of synthetic glucocorticoids is to increase feed intake in livestock for weight gain purposes. The hormone has been used in animal husbandry for 60 years. Under certain feeding conditions, it can improve the feed conversion rate, but at the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 肖光参邱彬林振宇郭隆华陈国南
Owner FUZHOU UNIV
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