Device and method for hydrolyzing plant hemicellulose by fermented citric acid wastewater

A technology for fermenting citric acid and plant cellulose, which is applied in the field of enzymatically decomposing plant cellulose to obtain sugar and hydrolyzing plant hemicellulose in the next step. It can solve the problem of high cost, reduce processing cost, reduce pretreatment cost, and reduce fermentation The effect of inhibitor production

Inactive Publication Date: 2010-07-28
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of organic acids is higher than that of commonly used inorganic acids. Therefore, it is necessary to find a cheap and easy-to-obtain organic acid to replace inorganic acids to hydrolyze plant cellulose.

Method used

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  • Device and method for hydrolyzing plant hemicellulose by fermented citric acid wastewater
  • Device and method for hydrolyzing plant hemicellulose by fermented citric acid wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This embodiment combines figure 1 Describe the structure of the device.

[0033]The device of the present invention that can effectively utilize fermented citric acid wastewater to hydrolyze plant hemicellulose is mainly composed of a circulating spray flushing filter reactor a, an acid storage tank 11, a water storage tank 10, heat exchangers 5 and 7, wherein the circulating spray The top inlet b of the flushing reactor a is connected to the acid storage tank 11 and the water storage tank 10 through the pump 9 on the one hand, and connected to the circulating spray flushing reactor through the first heat exchanger 7 and the pressure valve 8 on the other hand. The outlet c at the bottom of a is circulated; the outlet c at the bottom of the circulating spray flushing reactor a is also connected with a second heat exchanger 5 .

[0034] Wherein, the upper side of the circulating spray flushing filter reactor a is provided with a material inlet d, and the lower side is pr...

Embodiment 2

[0037] This embodiment illustrates the detailed steps of implementing the method of the present invention by using the device of the present invention.

[0038] Wherein, the xylose detection method in the xylose hydrolyzate obtained after the hydrolysis of the hemicellulose involved in the embodiment is: HPLC (Dionex), Bio-Rad Aminex HPX-87H column, differential detector; column temperature: 65 ° C, Mobile phase 0.005mol / L sulfuric acid, flow rate: 0.6mL / min.

[0039] The detection method of glucose in the enzymolysis solution obtained after cellulose enzymolysis is: HPLC (Dionex), Bio-RadAminex HPX-87H column, differential detector. Column temperature: 65°C, mobile phase 0.005mol / L sulfuric acid, flow rate: 0.6mL / min.

[0040] (1) Pretreatment of fermented citric acid waste water: heat the fermented citric acid waste water after natural settlement to 90°C for evaporation and concentration; add concentrated sulfuric acid with a volume ratio of 0.1% and mix evenly, filter and ...

Embodiment 3

[0046] The detection method of xylose and glucose described in this embodiment is the same as that in Example 2.

[0047] (1) Pretreatment of fermented citric acid waste water: heat the fermented citric acid waste water after natural settlement to 100°C for evaporation and concentration; add concentrated sulfuric acid with a volume ratio of 0.01% and mix evenly, filter and place in acid storage tank 11 ready for use;

[0048] (2) Hydrolysis of hemicellulose: Air-dry and pulverize sorghum stalks to the last 10 mesh, add from material inlet d and place on the bottom sieve plate 4 of circulating spray flushing reactor a, ferment lemon from acid storage tank 11 The liquid-solid ratio of acid waste water and sorghum stalks is 20:1 (w / v) and injected into fermented citric acid waste water; the thermocouple 2 is controlled by the temperature controller 1 so that the temperature of the reactor a is 200°C, and the first heat exchanger 7 is controlled to maintain the same temperature. ...

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Abstract

The invention relates to a device capable of effectively utilizing fermented citric acid wastewater to hydrolyze plant hemicellulose. The device is mainly composed of a circulating spray flushing and filtering reactor, an acid storage tank, a water storage tank and a heat exchanger; the method is characterized by comprising the steps: at first, mixing the citric acid wastewater with raw material of the plant hemicellulose for hydrolysis reaction at 100 to 200 DEG C to obtain hydrolyzate mainly containing pentose, and then adding cellulase into hydrolyzed residues for batch enzyme hydrolysis to obtain high-concentration dextrose for the fermentation of ethanol. The method for preparing fermentable sugar for ethanol by pretreating the raw material of the plant hemicellulose by using the fermented citric acid wastewater can not only sufficiently utilize the fermented citric acid wastewater to save water resource, but can also lower the cost for treating wastewater from fermented citric factory and the production cost of the fermentable sugar for cellulose ethanol.

Description

technical field [0001] The invention relates to a device and method for hydrolyzing plant hemicellulose by fermenting citric acid wastewater, and a process for obtaining sugar by enzymolyzing plant cellulose in the next step derived therefrom, belonging to the field of biochemical engineering. Background technique [0002] The wastewater produced after industrial fermentation of citric acid is characterized by high concentration and low pH. Traditional methods of treating fermented citric acid wastewater generally use anaerobic, aerobic biological methods or biological combination methods, etc., but these methods not only require large towers and pools and other equipment, but also the treated wastewater still needs to be discharged, polluting the environment And waste a lot of water resources. [0003] For the pretreatment method of plant cellulose, dilute acid hydrolysis is one of the common pretreatment methods. Compared with other methods (such as steam explosion, alkal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/14C12P19/14
Inventor 黄和张红漫金强赵晶林增祥陈敬文郑荣平
Owner NANJING UNIV OF TECH
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