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Method for extracting synanthrin from burdock

A technology of burdock and inulin, which is applied in the field of extracting inulin from burdock to achieve the effects of improving utilization efficiency and saving economic costs

Inactive Publication Date: 2013-03-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods for extracting polysaccharides from plants at home and abroad include hot water extraction, alcohol precipitation, alkali extraction, acid extraction, enzyme extraction, and microwave extraction. , ultrasonic extraction, CO2 supercritical extraction, ultrafiltration, etc., but there is no report on the use of immobilized enzymes to extract burdock inulin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The fresh burdock root is washed with tap water, dried to constant weight at 40°C, crushed, passed through a 40-mesh sieve, and formulated with acetic acid-sodium acetate buffer to form a solution with a pH of 4. Dissolve cellulase (50,000 U / g) and papain (30,000 U / g) in acetic acid-sodium acetate buffer at pH 4 at a molar ratio of 1:1 to prepare a 4 mg / mL solution. Fix in LX-1000EP resin at 30°C. Then, the immobilized enzyme and the burdock solution were mixed according to the solid-liquid ratio of 1:15, and the temperature was 30°C and the pH was 4, and the temperature was shaken for 4 hours. Filtrate to obtain the enzymatic hydrolysis solution, separate by ultrafiltration membrane, concentrate and dry to obtain the burdock inulin extract.

Embodiment 2

[0019] The fresh burdock root was washed with tap water, dried at 45°C to constant weight, crushed, passed through a 40-mesh sieve, and formulated with acetic acid-sodium acetate buffer to form a solution with a pH of 4.5. Cellulase (50,000 U / g) and papain (30,000 U / g) were dissolved in acetic acid-sodium acetate buffer solution with a pH of 4.5 at a molar ratio of 1:1 to prepare a 5 mg / mL solution. Fix in LX-1000EP resin at 40°C. Then, the immobilized enzyme and the burdock solution were mixed according to the solid-liquid ratio of 1:15, and the temperature was 35°C and the pH was 4.5, and the temperature was shaken for 4 hours. Filtrate to obtain the enzymatic hydrolysis solution, separate by ultrafiltration membrane, concentrate and dry to obtain the burdock inulin extract.

Embodiment 3

[0021] Fresh burdock root is washed with tap water, dried at 50°C to constant weight, crushed, passed through a 40-mesh sieve, and formulated with acetic acid-sodium acetate buffer to form a solution with a pH of 5. Cellulase (50,000 U / g) and papain (30,000 U / g) were dissolved in acetic acid-sodium acetate buffer solution with a pH of 5 at a molar ratio of 1:1 to prepare a 6 mg / mL solution. Fix in LX-1000EP resin at 50°C. Then, the immobilized enzyme and the burdock solution were mixed according to the solid-liquid ratio of 1:15, and the temperature was 35°C and the pH was 5, and the temperature was shaken for 4 hours. Filtrate to obtain the enzymatic hydrolysis solution, separate by ultrafiltration membrane, concentrate and dry to obtain the burdock inulin extract.

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PUM

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Abstract

The invention belongs to the field of extraction methods of synanthrin, and in particular relates to a method for extracting the synanthrin from burdock. The method for extracting the synanthrin from the burdock comprises the following steps in sequence: cleaning fresh burdock; adding immobilized cellulose and immobilized papain to mix with burdock solution; and oscillating at constant temperature by an oscillator. The method for extracting the synanthrin from the burdock has the beneficial effects that the immobilized cellulose and immobilized papain are adopted and used for extracting the synanthrin from the burdock, and the immobilized cellulose and immobilized papain can be recycled, so that the utilization efficiency of the cellulose and the papain can be improved, and the economic cost can be saved.

Description

technical field [0001] The invention belongs to the field of extraction methods of inulin, and in particular relates to a method for extracting inulin from burdock. Background technique [0002] Burdock, also known as tree root vegetable, also known as Dalizi, is a large-scale herbaceous plant with tap root system in the genus Arctium of Compositae. Rich in various vitamins and minerals such as calcium, magnesium, copper and manganese, as well as organic substances such as arctiin, arctol, arctipicrin and inulin, which have the functions of invigorating the stomach, nourishing qi, diuresis, purging, nourishing yin and strengthening yang , heat-clearing and detoxifying, and antibacterial and anti-inflammatory effects, it is deeply loved by people in Japan, Southeast Asia and other countries. In China, burdock root, as a folk herbal medicine, is not recorded in the Pharmacopoeia, and rarely becomes a commodity. With people's continuous in-depth understanding of its nutritio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 张成如
Owner SHANDONG UNIV
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