Bacillus siamensis capable of degrading grease and application of bacillus siamensis in grease-containing wastewater

A bacillus and oil technology, applied in the field of environmental microorganisms, can solve problems such as oil degradation of Bacillus siamese that have not yet been reported

Active Publication Date: 2021-10-01
FOSHAN YUHUANG ECOLOGICAL ENVIRONMENT TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many types of microorganisms that have the function of degrading oil, such as Pseudomonas, Klebsiella oxytoca, Staphylococcus saprophyticus, Serratia marcescens, yeast, mold, etc. Bacillus for Degradation of Oil in Wastewater

Method used

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  • Bacillus siamensis capable of degrading grease and application of bacillus siamensis in grease-containing wastewater
  • Bacillus siamensis capable of degrading grease and application of bacillus siamensis in grease-containing wastewater
  • Bacillus siamensis capable of degrading grease and application of bacillus siamensis in grease-containing wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Screening, performance verification and identification of Bacillus siamese BS1

[0059] The oil-degrading Bacillus siamese BS1 according to the present invention is screened and collected from kitchen waste oil-containing wastewater.

[0060] enrichment culture:

[0061] Take 5 mL of the collected sample, add it to an Erlenmeyer flask containing 100 mL of enriched medium, and place it on a constant temperature culture shaker for cultivation.

[0062] Composition of enrichment medium: soybean oil 10 g / L (soybean oil brand: Arowana, the soybean oil used below is the same brand), trypsin 5 g / L, yeast powder 10 g / L, sodium chloride 10 g / L L; adjust the pH to 6.8.

[0063] The culture conditions were 30 °C and 200 rpm, and the culture was terminated after 36 h. After the end, take out 5 mL of culture and inoculate it in fresh enriched medium for subculture. The final culture is used to isolate target microorganisms.

[0064] Isolation and Purification:

[00...

Embodiment 2

[0086] Example 2: Performance of Bacillus siamese BS1 at different temperatures

[0087] Bacillus siamese BS1 was inoculated into the oil medium containing 2 g / L soybean oil, the culture temperature was controlled at 30 °C, and the speed of the constant temperature culture oscillator was 200 rpm, and the culture was completed for 12 h. The resulting culture was used as seed for subsequent validation.

[0088] The cultured seeds were inoculated in 100 mL of oil medium containing soybean oil with a concentration of 10 g / L according to the inoculation amount of 5%. . Then, the Erlenmeyer flasks were placed in a constant temperature culture shaker with temperatures of 15 °C, 25 °C, 30 °C, 37 °C, 40 °C, and 45 °C, respectively, and the speed of the constant temperature culture shaker was controlled at 200 rpm. During the culture process, 1 mL samples were taken every 6 h until the end of 48 h. The 1 mL sample taken was detected by a spectrophotometer with the absorbance value OD...

Embodiment 3

[0090] Example 3: Performance of Bacillus siamese BS1 under different dissolved oxygen

[0091] Bacillus siamese BS1 was inoculated into the oil medium containing 2 g / L soybean oil, the culture temperature was controlled at 37 °C, and the speed of the constant temperature culture oscillator was 200 rpm, and the culture was completed for 12 h. The resulting culture was used as seed for subsequent validation.

[0092] The cultured seeds were inoculated in 100 mL of oil medium containing soybean oil with a concentration of 10 g / L according to the inoculation amount of 5%. . Then, the Erlenmeyer flasks were placed in a constant temperature culture shaker with a rotating speed of 100 rpm, 150 rpm, 200 rpm and 250 rpm, respectively, at 37 °C, and the speed of the constant temperature culture shaker was controlled at 200 rpm. During the culture process, 1 mL samples were taken every 6 h until the end of 48 h. The 1mL sample was taken and the absorbance value OD at 600 nm was detec...

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Abstract

The invention discloses bacillus siamensis capable of degrading grease and the application of the bacillus siamensis in grease-containing wastewater. The bacillus siamensis BS1 disclosed by the invention has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2021840 in the China Center for Type Culture Collection). The bacillus siamensis BS1 disclosed by the invention can be used for degrading grease. In a grease culture medium containing soybean oil with the initial concentration of 9.5-10.5 g / L, after being cultured for 48 hours, the grease degradation rate reaches 82.34%, wherein the culture temperature is controlled at 37 DEG C, and the rotating speed of a constant-temperature culture oscillator is 200 rpm. The invention also discloses the application of the bacillus siamensis BS1 in grease-containing wastewater, and the bacillus siamensis BS1 has a relatively good grease degradation capability.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a strain of Bacillus siamese that degrades grease and its application in grease-containing wastewater. Background technique [0002] With the improvement of people's living standards and the rapid development of the catering industry, the content of oils and fats in catering has increased, and the discharge of catering wastewater has also increased. The grease contained in the wastewater is discharged into the environment, which will pollute the environment, form an oil film on the water surface, affect the dissolved oxygen in the water body, and thus endanger aquatic organisms; at the same time, grease is an organic matter, which will lead to eutrophication of the water body when discharged into the environment; , The viscosity of the grease is relatively large, and it is easy to adhere to the inner wall of the waste water transportation pipeline...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F101/34C02F103/32
CPCC12N1/20C02F3/34C02F2101/34C02F2103/322
Inventor 邱建贺翁雯梁锡伟周国彪王春莲陈健辉
Owner FOSHAN YUHUANG ECOLOGICAL ENVIRONMENT TECH
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