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Brasenia schreberi polysaccharide extraction method

A technology for polysaccharide extraction and water shield, which is applied in the field of plant polysaccharide extraction, can solve the problems of low polysaccharide yield and low polysaccharide rate of water shield, and achieve high yield, good effect and high utilization rate

Inactive Publication Date: 2015-02-11
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for extracting water shield polysaccharides, which solves the problem that the yield of polysaccharides extracted by the traditional extraction method of water shield polysaccharides is low, and the yield of water shield polysaccharides is low.

Method used

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  • Brasenia schreberi polysaccharide extraction method
  • Brasenia schreberi polysaccharide extraction method
  • Brasenia schreberi polysaccharide extraction method

Examples

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Effect test

Embodiment 1

[0064] Example 1, the solid-liquid ratio is 1:30 (g / mL), the alkali concentration is 0.1mol / L, the oscillation frequency is 180r / min, the extraction time is 2 hours, the extraction temperature is 30°C, and the extraction is performed 4 times;

Embodiment 2

[0065] Example 2, considering other factors such as energy, efficiency and cost, the extraction process of water shield polysaccharides is selected as a solid-liquid ratio of 1:24 (g / mL), an alkali concentration of 0.1mol / L, an oscillation frequency of 180r / min, and an extraction time of 2 hours , extraction temperature 30 ℃, extraction 4 times.

Embodiment 3

[0066] Example 3, the solid-liquid ratio is 1:25 (g / mL), the alkali concentration is 0.09mol / L, the oscillation frequency is 175r / min, the extraction time is 2 hours, the extraction temperature is 30°C, and the extraction is performed 4 times.

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Abstract

The invention discloses a brasenia schreberi polysaccharide extraction method. According to the method, freeze-dried brasenia schreberi is pulverized, petroleum ether is adopted for extraction degreasing at 50 DEG C, after solvents are dried through volatilization at the room temperature, the crushed brasenia schreberi is placed in a drying box to be dried at 40 DEG C, the dried brasenia schreberi is obtained, a NaOH solution is added, oscillation extraction is carried out on a water bath oscillator, then, the centrifugal treatment is carried out, supernatant containing brasenia schreberi polysaccharide is obtained, the supernatant is extracted out, brasenia schreberi powder precipitates are left, the NaOH solution is added for continuous extraction, the number of extraction times is totally four, the supernatant obtained in each time is merged, the final supernatant containing polysaccharidec is obtained, and the polysaccharidec yield is measured. The brasenia schreberi polysaccharide extraction method has the beneficial effects that the utilization rate of brasenia schreberi raw materials is high, the effect is good, and the yield of brasenia schreberi polysaccharide extracted by the method provided by the invention is high.

Description

technical field [0001] The invention belongs to the technical field of methods for extracting polysaccharides from plants, and relates to a method for extracting water shield polysaccharides. Background technique [0002] Water shield is a traditional rare aquatic vegetable in my country, which has rich nutritional value and medicinal value. According to the "Compendium of Materia Medica", water shield has the effects of quenching thirst and heat, curing heat jaundice, thickening the stomach, calming the lower energizer, dispelling water, and detoxifying. . Chongqing Shizhu water shield grows in the Tujia Autonomous County in the hinterland of the Three Gorges Reservoir area in the southeast of Chongqing. The average altitude is above 1,500 meters, the water quality is good, the soil is fertile, and the annual output of water shield reaches 1,000 tons. It is the largest water shield base in my country, Asia, and the world. Studies have shown that Brasenia polysaccharide has ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张宇昊马良刘馨
Owner SOUTHWEST UNIVERSITY
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