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Target protein separation and purification system

A technology for separation and purification of target proteins, applied in the field of biochemical separation, which can solve the problems of adding too much or a small amount of buffer, improving and unfavorable extraction rate of target protein, etc., to achieve the effect of improving extraction rate, ensuring stability and ensuring accuracy

Inactive Publication Date: 2021-08-10
上海点拾科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

However, the existing target protein separation and purification system for water bloom cyanobacteria cannot quantitatively process the added buffer, which can easily lead to excessive or small amounts of buffer added, resulting in low accuracy of the amount of buffer added. The separation and purification of protein is inconvenient, and it is not conducive to the improvement of the extraction rate of the target protein

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  • Target protein separation and purification system
  • Target protein separation and purification system
  • Target protein separation and purification system

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Embodiment Construction

[0025] The following is attached Figure 1-4 The principles and features of the present invention are described, and the examples given are only used to explain the present invention, and are not used to limit the scope of the present invention. In the following paragraphs the invention is described more specifically by way of example with reference to the accompanying drawings. Advantages and features of the present invention will be apparent from the following description and claims. It should be noted that all the drawings are in a very simplified form and use imprecise scales, and are only used to facilitate and clearly assist the purpose of illustrating the embodiments of the present invention.

[0026] It should be noted that when a component is said to be "fixed" to another component, it can be directly on the other component or there can also be an intervening component. When a component is said to be "connected" to another component, it may be directly connected to ...

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Abstract

The invention relates to a target protein separation and purification system which comprises an ultrasonic oscillator, a base plate is arranged at the bottom of the ultrasonic oscillator, two sets of vertical rods are connected to the rear side of the top of the base plate, the tops of the two sets of vertical rods are jointly connected with a top plate, and a control panel is arranged on the side portion of the top plate. A display screen is arranged on the control panel, a lifting mechanism is arranged on the top plate, a fixing ring is arranged on the lifting mechanism, a burette is movably inserted into the inner cavity of the fixing ring, scales are arranged on the burette, a limiting ring is fixedly connected to the periphery of the burette in a sleeving mode, and a monitoring mechanism is arranged in an inner cavity of the burette. An electromagnetic valve is arranged on the burette, and a titration nozzle is communicated with the bottom of the burette. According to the system, when the crushed cells are quantitatively titrated with the buffer solution, the accuracy of the titration amount of the buffer solution is ensured, and the extraction rate of the target protein in the water-blooming cyanobacteria is favorably improved.

Description

technical field [0001] The invention relates to the technical field of biochemical separation, in particular to a target protein separation and purification system. Background technique [0002] Bloom cyanobacteria are rich in phycobiliproteins, and the structures of different phycobiliproteins are basically similar. They all contain two polypeptide chains α and β subunits with similar structures. The molecular weight is about 30,000 Daltons. Each subunit is related to a Molecule of phycocyanin binding. According to the absorption spectrum properties, phycobiliproteins can be divided into phycocyanin, allophycocyanin, phycoerythrin and phycoerythrin. Among them, phycocyanin and allophycocyanin generally exist in the form of trimers and hexamers. The higher the purity of phycocyanin and allophycocyanin, the higher the price. Extracting and purifying high-purity phycocyanin and allophycocyanin from cyanobacteria in water blooms can realize the harmless treatment of cyanobact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K14/795
CPCC07K1/14C07K14/795
Inventor 骆国众
Owner 上海点拾科技有限公司
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