Magnetic nano composite material for multimode peptide fragment enrichment and preparation method and application thereof

A technology of magnetic nanometer and composite materials, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., to achieve the effects of good magnetic response performance, high magnetic saturation strength, and uniform particle size

Active Publication Date: 2019-03-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the magnetic metal-organic framework nanocomposites are single-mode for the enrichment of phosphorylated polype...

Method used

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  • Magnetic nano composite material for multimode peptide fragment enrichment and preparation method and application thereof
  • Magnetic nano composite material for multimode peptide fragment enrichment and preparation method and application thereof
  • Magnetic nano composite material for multimode peptide fragment enrichment and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of superparamagnetic Fe by hydrothermal method 3 o 4 nano magnetic ball

[0042] The Fe used in the following examples 3 o 4 The specific preparation process of the magnetic ball is: the raw material 1.157gFeCl 3 ·6H 2 O, 3.303gNH 4 Ac (ammonium acetate) and 0.4gNa 3 CT (sodium citrate) was added to a reaction kettle containing 60mL of ethylene glycol, and magnetically stirred for 1 hour to mix the above materials evenly; then the stirrer was removed, and the temperature of the reaction kettle was raised to 200°C for 16 hours of reaction; then the reaction kettle Cool to room temperature, magnetically separate the reaction solution and collect the isolated solid product; then wash the solid product with ethanol and deionized water successively (each washing solution is washed five times), to obtain Fe 3 o 4 magnetic ball.

[0043] The Fe obtained by this method 3 o 4 Magnetic spheres can be uniformly dispersed in water to form a stable s...

Embodiment 2- Embodiment 10

[0045] Example 2-Example 10Fe 3 o 4 Magnetic spheres wrapped in silicon dioxide (SiO 2 ) layer (300nm Fe 3 o 4 magnetic ball)

[0046] Take the raw material according to table 1, and prepare Fe according to the following method in conjunction with the process parameters given in table 1 3 o 4 / SiO 2 Nanoparticles:

[0047] (1) Add 20 mL of HCl with a concentration of 0.1 M to a container containing 100 mg of Fe 3 o 4 In a 100mL two-necked bottle with magnetic balls, perform magnetic separation after ultrasonic treatment for 30 minutes, and collect the separated Fe 3 o 4 Magnetic spheres, isolated Fe 3 o 4 The magnetic ball is washed three times with deionized water to complete the Fe 3 o 4 Pretreatment of magnetic spheres.

[0048] (2) The pretreated Fe 3 o 4 Magnetic balls were added to a mixture of ethanol and deionized water at a volume ratio of 4:1 to obtain Fe 3 o 4 magnetic sphere suspension, and then under ultrasonic conditions to Fe 3 o 4 Add 0.5-2...

Embodiment 11- Embodiment 18

[0052] Embodiment 11-embodiment 18 prepares Fe 3 o 4 / SiO 2 / MOF Magnetic Nanocomposites

[0053] The following examples adopted a one-pot method, and the reaction device used was a two-necked flask reaction vessel heated by an oil bath and mechanically stirred.

[0054] 116mg Fe 3 o 4 / SiO 2 The nanoparticles were uniformly dispersed in 2mL DMF to obtain Fe 3 o 4 / SiO 2 Nanoparticle suspension, dissolving 116-348mg zirconium chloride in 4mL DMF to obtain zirconium chloride solution, dissolving terephthalic acid in 4mL DMF to obtain terephthalic acid solution, dissolving terephthalic acid in 4mL DMF to obtain terephthalic acid Diboronic acid solution. Zr in zirconium chloride solution 4+ The ratio of the amount of the substance to the sum of the amount of terephthalic acid and terephthalic diboronic acid in the terephthalic acid solution and the terephthalic diboronic acid solution is 1:1; terephthalic acid (PTA) and terephthalic diboronic acid The molar ratio of (P...

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Abstract

The invention discloses a magnetic nano composite material for multimode peptide fragment enrichment and a preparation method and application thereof. The magnetic nano composite material is composedof Fe3O4 magnetic sphere, a SiO layer coating the surface of the Fe3O4 magnetic sphere and a metal-organic frame layer growing on the SiO2 layer, the metal-organic frame layer is formed by metal ion Zr4+ and two organic ligands through coordination, and the organic ligands are terephthalic acid and phenylenebisboronic acid. The magnetic nano composite material takes the Fe3O4 magnetic sphere as acore, thereby having good magnetic response performance, and takes Zr4+ as an affine site of phosphorylated polypeptide and boric acid group on terephthalic acid as an affine site of glycopeptide, thereby being capable of realizing gathering of the phosphorylated polypeptide and gathering of the glycopeptides, high in gathering efficiency, of very important significance in studying physiological behavior protein phosphorylation and glycosylation and good in application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and relates to a magnetic nanocomposite material capable of simultaneously enriching multiple model peptides, a preparation method and an application thereof. Background technique [0002] Magnetic metal-organic framework nanocomposites are composed of magnetic nanospheres and metal-organic frameworks (Metal-Organic Frameworks, MOFs) wrapped on the surface of magnetic nanospheres. Therefore, in recent years, magnetic metal-organic framework nanospheres have attracted much attention and have been widely used in the field of biomedicine, especially in the separation of proteins or peptides. , drug delivery, and magnetic resonance imaging. [0003] At present, the magnetic metal-organic framework nanocomposites are single-mode for the enrichment of phosphorylated polypeptides and glycopeptides, that is, they can only be used for the enrichment of one type of peptide (phosphorylated polypeptide...

Claims

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Application Information

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IPC IPC(8): B01J20/22B01J20/30B01J20/28C07K1/22
CPCB01J20/06B01J20/103B01J20/226B01J20/28007B01J20/28009B01J20/28016B01J2220/46C07K1/22
Inventor 吴尧罗斌蓝芳
Owner SICHUAN UNIV
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