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Method and SSR primers for purity identification of melon hybrid Huangjinmi 25

A technology for purity identification and hybrids, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. It can solve problems such as large seasonal restrictions and long identification periods, and achieve convenient operation and high commercial applications. Value, effect of genetic stability

Inactive Publication Date: 2019-03-08
江苏绿港现代农业发展股份有限公司
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  • Abstract
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Problems solved by technology

[0004] The present invention aims at the defects that current melon hybrid seed purity identification mostly relies on field morphological identification methods, such as large seasonal restrictions and long identification period, etc. The first purpose of the present invention is to provide 2 pairs of hybrid seeds for identification of the new melon variety Golden Honey 25 Pure SSR primers, these two pairs of primers can produce specific markers in the parents of Golden Honey 25, with strong specificity

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  • Method and SSR primers for purity identification of melon hybrid Huangjinmi 25
  • Method and SSR primers for purity identification of melon hybrid Huangjinmi 25

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Embodiment Construction

[0032] The present invention will be described in further detail below in conjunction with specific embodiments, but the embodiments of the present invention are not limited thereto, and the protection scope of the present invention cannot be limited by the following embodiments.

[0033] Unless otherwise specified, the experimental methods used in the following embodiments are conventional methods; unless otherwise specified, the materials and reagents used can be obtained from commercial sources.

[0034] (1) Materials and methods

[0035] 1. Materials

[0036] Golden honey 25, early-maturing thick-skinned melon, it takes 30-35 days for the female flowers to open to fruit maturity; the female flowers open more neatly, saving labor and labor for pollination and cultivation management; the plants grow vigorously, the leaves are notched, and the light transmittance is good, which is conducive to fruit turning Ripe color and sugar accumulation; the fruit is oval, with golden to...

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Abstract

The invention discloses SSR primers for purity identification of melon hybrid Huangjinmi 25. The SSR primers include a primer pair gSSR15606 and / or a primer pair Gssr45335 and can generate specific markers in Huangjinmi 25 parents, and high specificity is realized. The invention further provides a method for purity identification of melon hybrid Huangjinmi 25. According to the method, melon hybridseed purity can be identified quickly, accurately and efficiently by the SSR primers. By the primer and the method, purity of the melon hybrid seeds can be effectively identified only through simpleextraction of melon leaf genome DNA, PCR amplification and electrophoresis. Hybrid seed purity identification can be completed within 5h according to the method, and the method has advantages of quickness, accuracy, low cost, convenience in operation and the like, can be used for substituting a traditional hybrid seed purity identification method and is high in commercial application value.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to an SSR primer and a method for identifying the purity of the melon hybrid Jinmi 25. Background technique [0002] Melon is an alienated pollination crop of Cucurbitaceae (Cucurbitaceae) and the genus Cucumis. It is an important fruit vegetable crop in the world and one of the top ten fruits in the world. Melon has strong heterosis, and the first-generation hybrids have the advantages of strong growth, good disease resistance, high yield, and good quality. In recent years, most of the commercial seeds used in melon production are hybrids. Since most hybrids are artificially produced, the purity is difficult to control. If detasseling is not timely and thorough, false hybrids are easily produced, resulting in reduced purity of hybrid seeds, failing to meet seed quality standards, and reducing crop yield and quality. [0003] The traditional method for the pur...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 阿门卢霞滕录华邓志军林爱华樊文义司龙亭
Owner 江苏绿港现代农业发展股份有限公司