UPLC (High Performance Liquid Chromatography) characteristic spectrum construction method and detection method for medicinal material plantain herb

Abstract

The invention relates to a UPLC (High Performance Liquid Chromatography) characteristic spectrum construction method and a detection method for a medicinal material plantain herb. The steps of the characteristic spectrum construction method are as follows: preparing a reference substance solution of the reference material by using the plantamajoside and the mullein glycoside as reference materials, respectively; preparing the reference substance solution of the reference medicinal material with the plantain herb as the reference material; respectively taking the medicinal material plantain herb and the standard decoction sample, extracting by adding extraction solvent, filtering, and applying the obtained filtrate as a test solution and a standard test solution in decoction form; respectively drawing the reference solution, the test solution, and the standard test solution in decoction form, injecting to a ultra-high performance liquid chromatograph for determination; comparing the spectrum of the medicinal material plantain herb test sample and the spectrum of the standard test solution in decoction form, calibrating characteristic peaks of the 7 water-soluble components, and obtaining the UPLC characteristic spectrum of the medicinal material plantain herb. The characteristic spectrum can be used for qualitative and quantitative analysis of the quality of the medicinal material plantain herb, the quality of the traditional decoction of the plantain herb prepared by using the medicinal material is ensured; and the characteristic spectrum is also suitable for detecting other preparations containing plantain herb.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a construction method and a detection method of a UPLC characteristic map of plantain medicinal material. Background technique [0002] Plantain is the dry whole grass of Plantago asiatica L. or Plantago depressaWilld. Plantain was first seen in "The Book of Songs·Zhou Nan·芣苢", and "Shen Nong's Materia Medica" was listed as the top grade. "Compendium of Materia Medica" states: "People in Youzhou call it Buglossis, and toads like to hide under it, so Jiangdong is called toad clothing; the smell is sweet, cold, and non-toxic. It is mainly used to relieve pain, improve water passages and urinate, and eliminate dampness. Take it for a long time to lighten the body and endure old age. Men are injured, women are drenched and don’t want to eat. It nourishes the lungs, strengthens yin and nourishes essence, makes people have children, improves eyesight and cures red pain. Removes wind ...

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Problems solved by technology

[0004] The 2015 edition of "Chinese Pharmacopoeia" uses psyllium as the quality evaluation index of psyllium, and only controls the content of psyllium, which cannot fully reflect its quality, and the establishment of the characteristic map of cypresses reported in the current literature research, All of them are only for the raw materials of medicinal materials, and most of the index components are fat-soluble components as the research objects. They are mainly used for qualitative identification of authenticity, origin, and quality differences of Chinese medicinal materials. It is difficult to fully reflect the quality characteristics of plantain grass, and it cannot reflect the traditional Chinese medicine soup. The material basis characteristics of the agent

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