A method for extracting tea polyphenols from camellia oleifera

A technology of tea polyphenols and camellia oleifera, which is applied in the field of natural product chemistry, can solve problems affecting product purity, and achieve the effects of low production cost, high extraction rate, and high product purity

Active Publication Date: 2021-08-31
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the addition of metal ion complex precipitation in this extraction process, the added metal ion impurities will affect the purity of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for extracting tea polyphenols from camellia oleifera comprises the following steps:

[0029] (1) Raw material pretreatment: collect the branches and leaves of Camellia oleifera, wash them with clear water, and after drying, sterilize them with an ultraviolet sterilizing lamp for 10 minutes, and crush them to 10 meshes to obtain raw materials for extraction;

[0030] (2) Enzymolysis: the extraction raw material is added in the composite enzyme aqueous solution (the mass ratio of the extraction raw material and the composite enzyme aqueous solution is 1:2, the mass fraction of the composite enzyme aqueous solution is 20%, and the composite enzyme is composed of pectinase and cellulase by mass ratio 1:2), adjust the pH value to 3.8 with acetic acid / sodium acetate buffer solution, heat up to 50°C for enzymolysis for 1 hour, heat up to 90°C to inactivate the enzyme after the enzymolysis is completed, and collect the enzymatic solution;

[0031] (3) Wall-breaking ...

Embodiment 2

[0037] The method for extracting tea polyphenols from camellia oleifera comprises the following steps:

[0038] (1) Raw material pretreatment: collect the branches and leaves of Camellia oleifera, wash them with clean water, sterilize them with an ultraviolet sterilizing lamp for 20 minutes after drying, and crush them to 30 meshes to obtain raw materials for extraction;

[0039] (2) Enzymolysis: the extraction material is added in the composite enzyme aqueous solution (the mass ratio of the extraction raw material and the composite enzyme aqueous solution is 1:4, the mass fraction of the composite enzyme aqueous solution is 22%, and the composite enzyme is composed of pectinase and cellulase according to mass ratio 1:2.5), adjust the pH value to 4.2 with acetic acid / sodium acetate buffer solution, heat up to 55°C for enzymolysis for 2 hours, heat up to 95°C to inactivate the enzyme after the enzymolysis is completed, and collect the enzymatic solution;

[0040] (3) Wall-break...

Embodiment 3

[0046] The method for extracting tea polyphenols from camellia oleifera comprises the following steps:

[0047] (1) Raw material pretreatment: collect the branches and leaves of Camellia oleifera, wash them with clear water, and after drying, sterilize them with an ultraviolet sterilizing lamp for 12 minutes, and crush them to 20 meshes to obtain the extracted raw materials;

[0048](2) Enzymolysis: the extraction material is added in the composite enzyme aqueous solution (the mass ratio of the extraction raw material and the composite enzyme aqueous solution is 1:4, the mass fraction of the composite enzyme aqueous solution is 22%, and the composite enzyme is composed of pectinase and cellulase according to mass ratio 1:2), adjust the pH value to 4.0 with acetic acid / sodium acetate buffer solution, heat up to 52°C for enzymolysis for 2 hours, heat up to 95°C after enzymolysis to inactivate the enzyme, and collect the enzymatic solution;

[0049] (3) Wall-breaking extraction: ...

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PUM

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Abstract

The invention discloses a method for extracting tea polyphenols from camellia oleifera. The branches and leaves of camellia oleifera are cleaned, dried, broken, and subjected to enzymatic hydrolysis and wall-breaking leaching, and the extract is roughly filtered through a 100-200-mesh filter. Finally, using the characteristics of the molecular size of tea polyphenols, tea polyphenols were separated by ultrafiltration with a 5-8kDa ultrafiltration membrane. Aiming at the characteristics of high tea polyphenol content and special leaf structure of fragrant flower camellia, the present invention improves the extraction process of tea polyphenol, provides a low-cost, high-yield method for extracting tea polyphenol from fragrant flower camellia, and improves the The utilization rate of Camellia oleifera planting products reduces the cost of extracting tea polyphenols. The tea polyphenols in the tea polyphenol concentrate prepared by the preparation method of the invention account for more than 98% of the total amount of solutes, and the extraction rate of the tea polyphenols in the extraction raw materials can reach more than 70%.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for extracting tea polyphenols from camellia oleifera. Background technique [0002] The planting area of ​​camellia oleifera forests in Guangxi is about 6 million mu, ranking third in the country. It is one of the main producing areas of camellia oleifera in the country. In the process of pruning and pruning of camellia oleifera trees and transformation of low-yield forests every year, a large number of leaves are often used as waste Abandoned, a great waste of resources. As an oil plant, after oil extraction every year, the quantity of remaining camellia oleifera husks is also very large. If it can be effectively used, it will generate huge economic benefits. At the end of 2011, a species of Camellia oleifera was discovered in Nanning, Guangxi. After the study of morphological characteristics and phenological differences, ISSR molecular i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07G99/00
CPCC07G99/00
Inventor 陈海燕杨漓梁忠云李桂珍叶海芹温如斯
Owner GUANGXI FORESTRY RES INST
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