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Pepsinogen II (PGII) detection kit and production technology

A technology of pepsinogen and detection kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor stability, low measurement repeatability, and poor sensitivity, and achieve good stability, good measurement repeatability, and detection The effect of high sensitivity

Active Publication Date: 2019-03-19
NANJING AUBRIME ABM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the determination of pepsinogen Ⅱ (PG Ⅱ) in the prior art requires a relatively complicated liquid preparation process, and the storage time is greatly affected by environmental conditions, and the long-term storage stability is poor, the sensitivity is poor, and the measurement repeatability is low.

Method used

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  • Pepsinogen II (PGII) detection kit and production technology
  • Pepsinogen II (PGII) detection kit and production technology
  • Pepsinogen II (PGII) detection kit and production technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the production of pepsinogen II (PGII) detection kit comprises the following steps:

[0046] S1: Weigh each component of reagent R1, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0047]

[0048] S2: Dissolve latex microsphere activator with 50mmol / L MES buffer solution, prepare 710mg / ml latex microsphere activator, add 6ml latex microsphere activator to 10g polystyrene latex microspheres to activate, at 25 ℃ Under stirring reaction 45min, obtain the latex microsphere solution of activation;

[0049] S3: Dilute the pepsinogen II (PGII) antibody with 50mmol / L MES buffer to prepare a 2mg / ml pepsinogen II (PGII) antibody dilution, and slowly drop the pepsinogen II (PGII) antibody dilution Add it to the latex microsphere solution prepared in step S2, control the mass ratio of pepsinogen II (PGII) antibody and polystyrene latex microsphere to 0.03:1, stir an...

Embodiment 2

[0056] Embodiment 2: the production of pepsinogen II (PGII) detection kit comprises the following steps:

[0057] S1: Weigh each component of reagent R1, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0058]

[0059] S2: Dissolve the latex microsphere activator with the MES buffer solution of 50mmol / L, prepare the latex microsphere activator of 710mg / ml, add 0.2ml latex microsphere activator to 0.33g polystyrene latex microsphere and activate, in Stir and react at 25°C for 45 minutes to obtain an activated latex microsphere solution;

[0060] S3: Dilute the pepsinogen II (PGII) antibody with 50mmol / L MES buffer to prepare a 2mg / ml pepsinogen II (PGII) antibody dilution, and slowly drop the pepsinogen II (PGII) antibody dilution Add it to the latex microsphere solution prepared in step S2, control the mass ratio of pepsinogen II (PGII) antibody and polystyrene latex microsph...

Embodiment 3

[0067] Embodiment 3: the production of pepsinogen II (PGII) detection kit comprises the following steps:

[0068] S1: Weigh each component of reagent R1, add it into the solvent and stir, stir evenly, let it stand still, filter and remove impurities, and the content of each component is as follows:

[0069]

[0070] S2: Dissolve latex microsphere activator with 50mmol / L MES buffer solution, prepare 710mg / ml latex microsphere activator, add 20ml latex microsphere activator to 33g polystyrene latex microspheres to activate, at 25 ℃ Under stirring reaction 45min, obtain the latex microsphere solution of activation;

[0071] S3: Dilute the pepsinogen II (PGII) antibody with 50mmol / L MES buffer to prepare a 2mg / ml pepsinogen II (PGII) antibody dilution, and slowly drop the pepsinogen II (PGII) antibody dilution Add it to the latex microsphere solution prepared in step S2, control the mass ratio of pepsinogen II (PGII) antibody and polystyrene latex microsphere to 0.03:1, stir a...

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Abstract

The invention provides a pepsinogen II (PGII) detection kit. The pepsinogen I detection kit comprises a reagent R1 and a reagent R2 which are independent to each other, wherein the reagent R1 comprises the following components: 1 to 10g / L of a trihytdroxy methyl aminomethane buffering solution, 1 to 20g / L of sodium chloride, 0.1 to 10mg / L of Tween 20, 10 to 50g / L of polyethylene glycol 20000 and 0.1 to 10g / L of sodium azide; the reagent R2 comprises the following components: 1 to 10g / L of the trihytdroxy methyl aminomethane buffering solution, 10 to 100g / L of trehalose, 2 to 10ml / L of a surfactant, 10 to 1000mg / L of a pepsinogen II (PGII)antibody and 0.1 to 10g / L of the sodium azide. The pepsinogen II (PGII) detection kit is ready to use and does not need to be prepared; and the pepsinogenII (PGII) detection kit has high detection sensitivity and good measurement repeatability and has relatively good stability when being stored for a long period. The invention further provides a production technology of the pepsinogen II (PGII) detection kit.

Description

technical field [0001] The invention relates to a pepsinogen II (PGII) detection kit, which belongs to the technical field of diagnostic kits. Background technique [0002] Pepsin is a digestive protease secreted by the principal cells of the gastric mucosa in the stomach. Its function is to break down protein in food into small peptide fragments. Pepsinogen (PG) is the inactive precursor of pepsin in gastric juice, which is divided into PGⅠ and PGⅡ. Pepsinogen is stimulated by gastric acid or pepsin to form pepsin. PG I is derived from the principal cells and cervical mucus cells of the fundic gland, while PG II is derived from the whole gastric gland (gastric cardia gland, gastric fundic gland, gastric antral pyloric gland) and distal duodenal Brunner's gland, and the prostate and pancreas also produce A small amount of PGⅡ, PGⅡ synthesized by gastric mucosa is about 25% of the total. Most of the synthesized PG enters the gastric cavity and is activated into pepsin under...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/539G01N33/573G01N33/577
CPCG01N33/539G01N33/573G01N33/577
Inventor 曾宪亮路爱成宣善浪
Owner NANJING AUBRIME ABM BIOTECH
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