Response surface method optimized method for extracting antioxidant ingredients from podocarpus leaves

A technology of antioxidant components and response surface method, applied in the direction of medical preparations containing active ingredients, plant/algae/fungus/moss components, anti-toxins, etc., can solve the problems of rare reports on the extraction of antioxidant components, and achieve extraction The effect of mild conditions and simple extraction process

Inactive Publication Date: 2019-03-22
JIAXING VOCATIONAL TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the extraction of the antioxidant components of Podocarpus

Method used

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  • Response surface method optimized method for extracting antioxidant ingredients from podocarpus leaves
  • Response surface method optimized method for extracting antioxidant ingredients from podocarpus leaves
  • Response surface method optimized method for extracting antioxidant ingredients from podocarpus leaves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Test method

[0030] 1 Extraction of antioxidant components

[0031] Accurately weigh 5.000g of Podocarpus pine leaf sample, extract with ultrapure water for a certain period of time at a certain ratio of solid to liquid, enzyme amount and extraction temperature, after centrifugation, dilute the resulting solution to a certain volume with ultrapure water volume, and shake well to obtain the sample solution.

[0032] 2 Determination of antioxidant activity

[0033] The antioxidant activity of the samples was measured by the DPPH free radical scavenging method. Determination of DPPH free radical scavenging of Podocarpus leaves extract with reference to Qin Manman et al. Pharmacology, 2014, 25(2):185-188), with modifications.

[0034] Accurately weigh 25.8mg of DPPH·, dissolve it with 70% ethanol and set the volume in a 200mL volumetric flask, store it in a brown bottle as a stock solution and store it in a refrigerator at 4°C, and take it out when used. Utilizing ...

Embodiment 2

[0070] A pectinase-assisted extraction method for the antioxidant components of Podocarpus leaves, the specific process is as follows:

[0071] Wash the fresh Podocarpus leaves, fix them for 5 minutes, dry them at 60°C, and grind them to get Podocarpus leaves powder.

[0072] Add ultrapure water to Podocarpus pine leaf powder to make the ratio of solid to liquid 1:20g / mL, pH 4.5, add pectinase at the enzymolysis temperature of 50°C for enzymolysis, enzymolysis time 2.5h, pectinase dosage It is 0.6g / L.

[0073] Filter the enzymatic solution with four layers of gauze, heat the filtrate to 90°C for 5 minutes to inactivate the enzyme, put it into a reagent bottle after cooling, seal it, and store it at 4°C.

[0074] After testing, the extraction rate of flavonoids and polysaccharides in the enzymolysis solution was 1.38% and 6.52%.

Embodiment 3

[0076] A pectinase-assisted extraction method for the antioxidant components of Podocarpus leaves, the specific process is as follows:

[0077] Wash the fresh Podocarpus leaves, fix them for 5 minutes, dry them at 60°C, and grind them to get Podocarpus leaves powder.

[0078] Add ultrapure water to Podocarpus pine leaf powder to make the ratio of solid to liquid 1:22g / mL, pH 4.5, add pectinase for enzymolysis under the condition of enzymolysis temperature 55℃, enzymolysis time 2.0h, pectinase dosage 0.5g / L.

[0079] Filter the enzymatic solution with four layers of gauze, heat the filtrate to 90°C for 5 minutes to inactivate the enzyme, put it into a reagent bottle after cooling, seal it, and store it at 4°C.

[0080] After testing, the extraction rate of flavonoids and polysaccharides in the enzymolysis solution was 1.23% and 6.31%.

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Abstract

The invention relates to a response surface method optimized method for extracting antioxidant ingredients from podocarpus leaves. The method comprises the following steps: a, weighing a podocarpus leaf powder, adding ultrapure water to perform enzymolysis extraction according to a fixed solid-liquid ratio under different temperatures, enzymolysis time and the addition amount of pectinase, and determining an optimal ratio by virtue of a single factor experiment and a response surface method; b, on the basis of the single factor experiment, taking the enzyme addition amount (A), the enzymolysistime (B) and the enzymolysis temperature (C) as independent variables, taking a scavenging rate (Y) of DPPH as a response value, and performing second-order polynomial model fitting of nonlinear regression by utilizing experimental design software; c, obtaining a response surface analysis chart according to a regression equation, investigating the fitted response surface shape, seeking the optimized process parameters by analyzing the response surface contour line, intuitively evaluating interactions of the various factors by analyzing the 3D surface chart, and finally obtaining the optimizedprocess parameters.

Description

technical field [0001] The invention relates to a method for extracting plant components, in particular to a method for extracting antioxidant components of Podocarpus leaves optimized by response surface methodology. Background technique [0002] Podocarpus, also known as Podocarpus, soil fir, scientific name Podocarpus macrophyllus (Thumb.) D.Don, a genus of gymnosperm Podocarpus family, is an evergreen tree. In recent years, scholars at home and abroad have studied the chemical composition and pharmacological effects of Podocarpus medicinal material. [1] Many studies have been carried out, and the biflavone components contained in Podocarpus plants have shown good activities in anti-tumor, anti-virus, anti-oxidation, anti-inflammation, and effects on the blood system and nervous system. Podocarpus seed extract has obvious inhibitory effect on human gastric cancer cell MGC-803 in vitro. Podocarpus fruit alcohol extract has the effects of lowering blood fat, protecting liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/15A61P39/06A61K127/00
CPCA61K36/15A61K2236/19A61P39/06
Inventor 蔡辉李素丹顾杰高诗佳沈杰
Owner JIAXING VOCATIONAL TECHN COLLEGE
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