Preparation method for taurine-modified novel giant squid gelatin multifunctional peptide

A technology of taurine and giant squid, which is applied in the field of preparation of new giant squid gelatin multifunctional peptides, can solve the problems of limited application, low rheological properties, and inability to completely replace mammalian gelatin, so as to promote brain development and production High efficiency and purity, high reaction efficiency and conversion

Inactive Publication Date: 2019-03-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with mammalian gelatin, fish gelatin has lower rheological properties, gelatinity, emulsification stability and other physical and chemical properties, so it cannot completely replace mammalian gelatin, which limits its application in food, medicine and other fields

Method used

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  • Preparation method for taurine-modified novel giant squid gelatin multifunctional peptide
  • Preparation method for taurine-modified novel giant squid gelatin multifunctional peptide
  • Preparation method for taurine-modified novel giant squid gelatin multifunctional peptide

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Embodiment 1

[0034] A preparation method of a novel giant squid gelatin multifunctional peptide modified with taurine, comprising:

[0035] 1) providing a carboxyl component derived from a protective peptide fragment whose amino acid sequence is SEQ ID NO.1;

[0036]2) providing an amino component, which is derived from taurine;

[0037] 3) providing a condensation reaction in the presence of a condensing agent to form a peptide bond between the carboxyl component and the amino component, and the above condensation reaction is carried out in the presence of formylglycine and p-aminophenylalanine;

[0038] 4) Provide a deamination protection reaction, which can remove the protection group at the amino terminal of the condensation reaction product. Taurine is a multifunctional active substance. By using taurine to modify the giant squid skin gelatin polypeptide with single antioxidant activity, a new type of multifunctional protein with both taurine biological activity and giant squid skin ...

Embodiment 2

[0049] A preparation method of a novel giant squid gelatin multifunctional peptide modified by taurine, comprising the following steps:

[0050] 1) Take the polypeptide and dissolve it in 15 times the amount of sodium carbonate solution with a concentration of 15%, then take 5 times the amount of fluorenylmethylcarbonyl chloride of the polypeptide, dissolve it in acetone with 10 times the amount of the polypeptide, and mix the above two solutions , stirred in an ice bath at -2°C for 30 min, then stirred at room temperature for 2 h, extracted 3 times with diethyl ether, adjusted the pH to 3 with concentrated hydrochloric acid, precipitated, extracted 3 times with ethyl acetate, dried over anhydrous magnesium sulfate Afterwards, use petroleum ether to separate out the precipitate to obtain the protective peptide fragment;

[0051] 2) Take the protective peptide fragment and diisopropylcarbodiimide at a weight ratio of 1:1.1, add tetrahydrofuran to dissolve, then add taurine, for...

Embodiment 3

[0054] A preparation method of a novel giant squid gelatin multifunctional peptide modified by taurine, comprising the following steps:

[0055] 1) Dissolve the polypeptide in 13 times the amount of sodium carbonate solution with a concentration of 12%, then take 4 times the amount of fluorenylmethylcarbonyl chloride of the polypeptide, dissolve it in acetone 11 times the amount of the polypeptide, and mix the above two solutions , stirred in an ice bath at 0°C for 25 min, then stirred at room temperature for 2.25 h, extracted twice with diethyl ether, adjusted the pH to 3 with concentrated hydrochloric acid, precipitated, extracted twice with ethyl acetate, dried over anhydrous magnesium sulfate Afterwards, use petroleum ether to separate out the precipitate to obtain the protective peptide fragment;

[0056] 2) Take protective peptide fragments and diisopropylcarbodiimide at a weight ratio of 1:1, add tetrahydrofuran to dissolve, then add taurine, formylglycine, and p-aminop...

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Abstract

The invention provides a preparation method for a taurine-modified novel giant squid gelatin multifunctional peptide and belongs to the technical field of biotechnology. The preparation method comprises the following steps: 1) supplying a carboxyl component which is from a protective peptide fragment with amino acid sequence of SEQ ID NO.1; 2) supplying an amino component which is from taurine; 3)supplying condensation reaction under the existence of condensing agent, and forming a peptide bond between the carboxyl component and the amino component, wherein the condensation reaction is performed under the existence of formylglycine and p-aminophenylalanine; 4) supplying amino deprotection reaction, thereby removing a protection group from an amino terminal on a condensation reaction product through the reaction. The preparation method for the novel multifunctional peptide provided by the invention has the advantages of high reaction speed, less synthesis time consumption, high conversion rate of reaction, low production cost, high activity and purity of products and few by-products. The prepared multifunctional peptide has biological activity functions of resisting against oxidation, removing free radicals, boosting brain development, and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of a novel giant squid gelatin multifunctional peptide modified with taurine. Background technique [0002] Gelatin is a high molecular natural polypeptide polymer produced by moderate hydrolysis of collagen. It is widely used in food, medicine and other fields because of its unique physical and chemical properties. At present, 98.5% of the commercial gelatin on the market mainly comes from the skin and bones of pigs and cattle. Due to the impact of shortage of raw materials and gelatin market, it is urgent to find a new type of gelatin raw material that can replace mammalian gelatin. [0003] Fish gelatin is considered to be one of the most potential mammalian gelatin substitutes due to its similar physicochemical properties to mammalian gelatin. However, compared with mammalian gelatin, fish gelatin has lower rheological properties, gelatinity, em...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/107
CPCC07K14/78Y02P20/55
Inventor 金火喜徐红萍曾明谢辉杨立业
Owner ZHEJIANG OCEAN UNIV
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