Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of Anji white tea polysaccharides and novel use of the polysaccharides against human cytomegalovirus

A technology of Anji white tea and polysaccharides, applied in the field of preparation of Anji white tea polysaccharides, can solve the problems of lack of drugs for HCMV infection, electrolyte imbalance, acidosis, etc.

Active Publication Date: 2019-03-29
ZHEJIANG HOSPITAL
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that electrolyte disturbance, acidosis, anemia, fever, and vomiting may occur during foscarnet treatment. Other anti-HCMV western medicines also have similar adverse reactions, such as bone marrow suppression, nephrotoxicity, and electrolyte disturbance. Emergence of induced drug-resistant strains
Therefore, there is still a lack of ideal drugs for the treatment of HCMV infection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of Anji white tea polysaccharides and novel use of the polysaccharides against human cytomegalovirus
  • Preparation method of Anji white tea polysaccharides and novel use of the polysaccharides against human cytomegalovirus
  • Preparation method of Anji white tea polysaccharides and novel use of the polysaccharides against human cytomegalovirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1: Preparation of Anji white tea polysaccharide

[0061] The old summer and autumn leaves of Anji white tea were dried in an oven at 70°C, crushed appropriately, immersed in absolute ethanol, and refluxed at 90°C for 2 hours to degrease. After suction filtration, the residue was collected, dried in a vacuum oven, and passed through a 40-mesh sieve. Weigh the sieved Anji white tea powder and put it into the extraction kettle, set the process conditions as follows: entrainer flow rate: 0.5mL min -1 ;CO 2 Flow rate: 2L·min -1 (CO 2 The outlet pressure of the storage tank is 5-6MPa, and the temperature is room temperature (25-30°C); outlet valve temperature: 70°C. The extraction time is 90min, the extraction pressure is 25.0MPa, the extraction temperature is 70°C, and the entrainer ethanol concentration is 90%. After dialysis and desalination of the extract, 3 times the amount of absolute ethanol was added, fully stirred and left overnight. The precipitate ...

Embodiment 2

[0062] Embodiment 2: HCMV inoculation and drug treatment

[0063] Using 30PD human embryonic lung diploid fibroblast WI-38, press 2×10 with 10% FBS medium 4 / cm 2 Inoculate into a culture dish, replace the medium with 0.2% FBS after 24 hours and continue to culture for 48 hours. Through this method of serum starvation, the cells are synchronized at G0 / G1 at this time, which is more conducive to the infection of HCMV. Afterwards, the HCMV virus (Towne virus strain) was inoculated with an inoculation amount of 0.01 MOI (multiplicity of infection), and the culture was continued until the specified time for relevant detection. When detecting the activity of anti-HCMV drugs, a certain concentration of drugs was added to the medium 2 hours in advance, and then HCMV was inoculated, and then the changes in cell morphology and changes in viral gene and DNA amplification were observed at different times.

Embodiment 3

[0064] Example 3: Effect of Anji white tea polysaccharides on the morphology of WI-38 cells after HCMV inoculation

[0065] After WI-38 was inoculated with HCMV, plaques began to appear on the 3rd day, and then gradually increased. On the 6th day after inoculation, the cells showed significant lesions, while the cells treated with Anji white tea polysaccharides, as the concentration of Anji white tea polysaccharides increased, Its pathological effect gradually weakened, similar to cells treated with the positive drug foscarnet (PFA) at higher doses, and no significant pathological changes ( figure 2 ).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biotechnology and relates to preparation of Anji white tea polysaccharides and use of the polysaccharides against a human cytomegalovirus (HCMV). The Anji white tea polysaccharides prepared by supercritical CO2 extraction have obvious anti-HCMV effect, and are characterized by significantly suppressing expression of HCMV characteristic genes IE1 / 2 and UL44 inits in vitro host cells that are human embryonic lung fibroblast, effectively suppressing DNA replication of HCMV, and significantly improving the cytopathic effect caused by HCMV infection. The Anjiwhite tea polysaccharides have low toxicity, and have the application prospect of preparing anti-HCMV drugs.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a preparation method of Anji white tea polysaccharide and its new application of anti-human cytomegalovirus. Background technique [0002] Human cytomegalovirus (HCMV) belongs to the β subfamily of herpesviruses. HCMV is the most common virus causing congenital infection worldwide, with an infection rate of 0.2% to 2% in live births. my country is also a high-incidence area of ​​HCMV infection. The clinical onset and outcome of HCMV infection are affected by the body’s immunity. Most HCMV-infected patients have no obvious symptoms, but in pregnant women, infants, the elderly with low immunity, and individuals with immunosuppression, it can cause serious diseases, such as fetal malformations, Infant CMV hepatitis, CMV pneumonia, multiple organ dysfunction, etc. HCMV infection is also one of the important reasons for the failure of organ transplantation. However, so far, there is no...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P31/22
CPCA61P31/22C08B37/0003Y02P20/54
Inventor 毛根祥张忠山王三应万晓青徐小刚苏慧丽王晓梅陈莎莎代继桓
Owner ZHEJIANG HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com