Gene synthesis duck alpha interferon gene and protein expression

An alpha interferon, whole gene technology, applied in the direction of interferon, cytokine/lymphokine/interferon, genetic engineering, etc., can solve problems such as loss of duck farming
CN101870976AInactive Publication Date: 2010-10-27NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2010-10-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention uses the gene synthesis technology to obtain the duck alpha interferon gene and carry out pronucleus expression on interferon protein, belonging to the field of biological pharmacy. The invention comprises the following steps: carrying out codon reformation to the duck alpha interferon gene sequence, and combining the sequence by genes, building the pronucleus expression carrier containing the duck alpha interferon gene; converting escherichia coli by the pronucleus expression carrier containing the duck alpha interferon gene, carrying out induction expression, extracting protein, carrying out denaturation and renaturation, and researching antiviral activity. The interferon obtained by the test can resist the attack of vesicular disease viruses, and the specific activity reaches 5.6*103U / mg. The interferon produced by the method has simple technology, high expression amount and high bioactivity.
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Description

a technical field

[0001] The invention belongs to the field of genetic engineering biopharmaceuticals, and relates to the prokaryotic expression of duck alpha interferon gene and interferon protein obtained by using the whole gene synthesis technology. Two background technology

[0002] At present, with the continuous expansion of the scale of duck raising in my country and the continuous improvement of the degree of intensification, the harm caused by duck viral diseases such as duck viral hepatitis, duck plague, and duck flu has become more and more serious, causing major damage to the duck industry. loss. The interferon system is an important defense system for the body to fight against viral infections. When the interferon system is activated, serum interferon can spread to various organs in the body within a few minutes, and it only takes a few minutes for cells to be exposed to interferon to produce antiviral State, moreover, animals can become resistant to superinfect...

Claims

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