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Method for simultaneously measuring hormones and antibiotics in fatty food sample

A technology for antibiotics and samples, which is applied to hormones and antibiotic compounds in complex matrices, and the field of simultaneous determination of hormones and antibiotics in fatty food samples, which can solve problems such as low efficiency, complex matrices, and drug loss, and achieve the effect of increasing diffusion

Active Publication Date: 2019-04-09
SHENYANG PHARMA UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to address the problems existing in the prior art, and to provide a method for extracting steroid hormones and antibiotics in complex matrices such as meat using rapid solvent extraction technology, which can effectively improve the extraction efficiency of steroid hormones and antibiotics, and the matrix Difficult problems such as loss of medicines in the experimental process caused by complexity
And solve the existing problems of complex matrix affecting the extraction effect and low efficiency

Method used

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  • Method for simultaneously measuring hormones and antibiotics in fatty food sample
  • Method for simultaneously measuring hormones and antibiotics in fatty food sample
  • Method for simultaneously measuring hormones and antibiotics in fatty food sample

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Embodiment 1

[0039] 1. Instruments and reagents

[0040] 1.1 Instruments:

[0041] ACQUITYTM UPLC system (Waters Company, Milford, Massachusetts, USA), electronic analytical balance, Zhongjia LC-400 low-speed centrifuge, Taixing freeze dryer, ASE 350 accelerated solvent extractor (Diane China Co., Ltd.), kq5200 ultrasonic cleaning machine (Kunshan Ultrasonic Instrument Co., Ltd.), rotary evaporator re-5203 (Shanghai Yalong Biochemical Instrument Factory)

[0042] Liquid chromatography

[0043] 1.2 Reagents:

[0044] Water: Class I water in accordance with GB / T 6682.

[0045] Methanol: analytically pure;

[0046] Acetonitrile: analytically pure;

[0047] n-Hexane: analytically pure;

[0048] Diatomaceous earth: 2mm;

[0049] 2. Method

[0050] 2.1 Preparation of reference solution

[0051] Take an appropriate amount of hormone and antibiotic reference substances, weigh them accurately, add methanol to make a 1mg / ml solution, and get it.

[0052] 2.2 Preparation of the test solutio...

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Abstract

The invention relates to the complex matrix extraction technology and the technical field of medicine ingredient detection, and relates to a method for simultaneously measuring hormones and antibiotics in a fatty food sample, in particular to a method for simultaneously measuring hormone and antibiotic compounds in complex matrixes, including pork and the like. By use of the method, a freezing dryer is used for carrying out freezing drying on the sample, then, the sample is grinded into powder, a quick solvent extraction instrument is used, the mixed solvent of normal hexane, methyl alcohol and acetonitrile is taken as extraction agent, and the sample is extracted, wherein the volume ratio of normal hexane, methyl alcohol and acetonitrile is 1:1:1-2:1:2. Meanwhile, a plurality of hormonesand antibiotics in the complex matrixes, including pork and the like, can be simultaneously extracted, and in addition, extraction efficiency is obviously superior to other extraction methods.

Description

【Technical field】 [0001] The invention relates to the technical fields of complex matrix extraction technology and drug component detection, and relates to a method for simultaneously measuring hormones and antibiotics in fatty food samples, in particular to a method for simultaneously measuring hormones and antibiotic compounds in complex matrices such as pork. 【Background technique】 [0002] Rapid Solvent Extraction (ASE) is based on the principle of different solubility of solutes in different solvents. Using rapid solvent extraction equipment, organic or polar solvents to achieve efficient and rapid extraction of organic compounds in solid or semi-solid samples. Fast solvent extraction is the latest extraction and separation method that integrates short extraction time, less solvent consumption, less environmental pollution, and automatic control. [0003] Steroid hormones can be produced by the body and play key roles and have important effects on physiology. Steroid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 赵龙山王梦婷王洋赵晶
Owner SHENYANG PHARMA UNIVERSITY
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