A female sex-specific molecular marker and genetic sex identification method of Chinese soft-shelled turtle

Abstract

The invention belongs to the technical field of aquatic organisms and discloses a specificity molecular marker for the female sex of the Chinese soft shell turtle and a genetic sex identification method. The method comprises the steps that a gene group DNA is subjected to enzyme digestion by a restriction enzyme; after an RAD sequencing result is filtered and subjected to quality control, all female samples and male samples are compared with a reference gene group, and according to a comparison result, all the female samples are filtered and screened to obtain reads comparison results; obtained scaffold is sequenced according to the comparison number of the total reads of the female samples, and DNA areas, covered with the reads, in all the samples are extracted and used as candidate female specific sequence fragments; according to the female specific sequence fragments, Walking of the female gene group is conducted, and the DNA fragments are obtained; a primer designed based on the specific fragments is subjected to primer screening and verification. The specificity molecular marker is high in genetic sex identification accuracy.

Description

technical field [0001] The invention belongs to the technical field of aquatic organisms, and in particular relates to a female sex-specific molecular marker of soft-shelled turtle and a genetic sex identification method. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Chinese soft-shelled turtle (Pelodiscus sinensis), commonly known as soft-shelled turtle, water fish, and turtle, belongs to the class Reptiles and the order Turtles. Its distribution range is wide, and it is distributed in my country except Qinghai, Tibet and Xinjiang. Because soft-shelled turtle has high nutritional value and medicinal value, it has always been loved by consumers. Male soft-shelled soft-shelled turtles grow faster than females. The skirts of males are wider and thicker, and the price of seedlings is higher than that of females. Breeding all-male or high-male soft-shelled soft-shelled turtles can increase breeding pr...

AI Technical Summary

Problems solved by technology

[0012] Accurate identification of the genetic sex of soft-shelled turtles can be achieved through the development of accurate and efficient molecular markers. Although there are many technical methods for the development of sex-specific markers, some of which are relatively mature, there are no technical obstacles in terms of technology. However, there are great uncertainties in the actual development of molecular markers, which are closely related to the Chinese soft-shelled turtle population used by researchers, molecular marker methods, analysis methods in the research process, parameter settings, etc.

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