A year-round production method for peony chromosomes

A technology of chromosome and peony, which is applied in the field of annual production of peony chromosomes, can solve the problems of unfavorable in-depth study of peony chromosomes and inability to preserve materials, and achieve the effects of low cost of production technology, short material collection cycle, and good dispersion

Inactive Publication Date: 2021-04-23
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromosome banding, fluorescence in situ hybridization and other techniques require a large number of repeated experimental materials. Although traditionally obtained materials can obtain uniformly dispersed and clear chromosome images, they cannot be preserved for a long time, which is not conducive to in-depth research on peony chromosomes.

Method used

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  • A year-round production method for peony chromosomes
  • A year-round production method for peony chromosomes
  • A year-round production method for peony chromosomes

Examples

Experimental program
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Effect test

Embodiment 1

[0059] The present embodiment relates to a method for preparing a paeoniae chromosome, comprising the steps of:

[0060] 1) Material collection: From 9:00 to 11:00 in the morning in mid-March 2017, Chinese peony planting plants were excavated in the peony germplasm resource nursery of Xiaotangshan Base, National Flower Engineering Technology Research Center, Beijing Forestry University, and the succulents that had germinated in early spring were obtained. The root tip of the young root system on the root is 1-2cm ( figure 1 ).

[0061] 2) Pretreatment: the obtained root tip material was placed in a centrifuge tube filled with saturated p-dichlorobenzene solution, pretreated at low temperature (4°C) for 10 hours, and then fixed for use.

[0062] 3) Fixation: Take the pretreated material out of the centrifuge tube, wash it with distilled water for 3 times, then place the material on filter paper to absorb the water, and finally transfer it to a centrifuge tube containing Carnot...

Embodiment 2

[0067] The present embodiment relates to a method for preparing a paeoniae chromosome, comprising the steps of:

[0068] 1) Materials collection: At the end of March, 2017, from 9:00 to 11:00 in the morning, unearthed and germinated mixed buds of Chinese peony varieties were dug out at the Xiaotangshan base, and the larger scales on the periphery were peeled off to obtain newly germinated bud tips ( image 3 ).

[0069] 2) Pretreatment: Put the bud tip in a saturated p-dichlorobenzene solution, and pretreat it at low temperature (4° C.) for 10 h.

[0070] 3) Fixation: Wash the pretreated bud tips 3 times with distilled water, then place the material on filter paper to absorb water, and finally transfer to Carnot’s fixative for fixation for more than 24 hours (4°C). Materials not used immediately after fixation were transferred to 70% ethanol solution for future use.

[0071] 4) Dissociation: Wash the fixed bud tips three times with distilled water, absorb the water with fil...

Embodiment 3

[0075] The shoot tip chromosome preparation after unearthed, the specific process is similar to embodiment 2, the difference is: in early April, 2017, at 9:00-11:00 in the morning, take the top shoot tip of the Chinese peony variety after it germinates from the soil and does not show buds, peel off To the peripheral scales ( Figure 5 ).

[0076] Optical microscopy results showed that ( Figure 6 ): 63 times microscopic examination, the chromosomes are clearly visible, the length is suitable, the staining effect is good, the cells are evenly dispersed, can be counted, and can be used for karyotype analysis. After counting, the chromosome 2n=10 of Chinese peony cultivars.

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Abstract

The present invention relates to a year-round film-making method of peony chromosome, comprising the following steps: (1) pretreatment: placing the material used for film-making in a saturated p-dichlorobenzene solution, and pre-treating it under the condition of 3-5°C Treatment for 9‑11 hours; (2) Fixation: transfer the pretreated material to Carnot’s fixative, and fix for more than 24 hours at 3-5°C; (3) Acid hydrolysis: transfer the fixed material to 0.95‑ In 1.05mol / L hydrochloric acid solution, dissociate at a temperature of 55-65°C for 9‑11min; (4) Stain and press. The present invention proposes a simpler and safer method suitable for the production of peony chromosomes throughout the year. This method can be applied to most of the production materials of peony through specific adjustments to the parameters of each step, and can be used throughout the year. The Chromosome of Paeoniae officinalis is prepared, which lays the foundation for the in-depth study of Paeoniae officinalis.

Description

technical field [0001] The invention relates to a method for producing chromosomes of plants, more specifically, to a method for producing chromosomes of peony throughout the year. Background technique [0002] Radix Paeoniae is a perennial herbaceous plant of Paeoniaceae Paeoniae genus, Paeoniae is divided into three groups: peony group, Paeoniae peony group and North American Paeoniae group, Paeoniae in the present invention refers to the wild species in the Paeoniae group or the varieties in and between groups. Peony is a traditional famous flower in my country and also a traditional flower of love in my country. It has a long history of cultivation and cultural heritage. The cultivated varieties of peony have large flowers, bright colors and rich flower patterns, and have various application forms such as garden cultivation, landscaping, fresh cut flowers, and medicinal purposes, and are loved and concerned by the people of our country and other countries in the world. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/30
Inventor 于晓南朱炜郑严仪赵芮程堂仁张启翔
Owner BEIJING FORESTRY UNIVERSITY
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