A year-round production method for peony chromosomes
A technology of chromosome and peony, which is applied in the field of annual production of peony chromosomes, can solve the problems of unfavorable in-depth study of peony chromosomes and inability to preserve materials, and achieve the effects of low cost of production technology, short material collection cycle, and good dispersion
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Embodiment 1
[0059] The present embodiment relates to a method for preparing a paeoniae chromosome, comprising the steps of:
[0060] 1) Material collection: From 9:00 to 11:00 in the morning in mid-March 2017, Chinese peony planting plants were excavated in the peony germplasm resource nursery of Xiaotangshan Base, National Flower Engineering Technology Research Center, Beijing Forestry University, and the succulents that had germinated in early spring were obtained. The root tip of the young root system on the root is 1-2cm ( figure 1 ).
[0061] 2) Pretreatment: the obtained root tip material was placed in a centrifuge tube filled with saturated p-dichlorobenzene solution, pretreated at low temperature (4°C) for 10 hours, and then fixed for use.
[0062] 3) Fixation: Take the pretreated material out of the centrifuge tube, wash it with distilled water for 3 times, then place the material on filter paper to absorb the water, and finally transfer it to a centrifuge tube containing Carnot...
Embodiment 2
[0067] The present embodiment relates to a method for preparing a paeoniae chromosome, comprising the steps of:
[0068] 1) Materials collection: At the end of March, 2017, from 9:00 to 11:00 in the morning, unearthed and germinated mixed buds of Chinese peony varieties were dug out at the Xiaotangshan base, and the larger scales on the periphery were peeled off to obtain newly germinated bud tips ( image 3 ).
[0069] 2) Pretreatment: Put the bud tip in a saturated p-dichlorobenzene solution, and pretreat it at low temperature (4° C.) for 10 h.
[0070] 3) Fixation: Wash the pretreated bud tips 3 times with distilled water, then place the material on filter paper to absorb water, and finally transfer to Carnot’s fixative for fixation for more than 24 hours (4°C). Materials not used immediately after fixation were transferred to 70% ethanol solution for future use.
[0071] 4) Dissociation: Wash the fixed bud tips three times with distilled water, absorb the water with fil...
Embodiment 3
[0075] The shoot tip chromosome preparation after unearthed, the specific process is similar to embodiment 2, the difference is: in early April, 2017, at 9:00-11:00 in the morning, take the top shoot tip of the Chinese peony variety after it germinates from the soil and does not show buds, peel off To the peripheral scales ( Figure 5 ).
[0076] Optical microscopy results showed that ( Figure 6 ): 63 times microscopic examination, the chromosomes are clearly visible, the length is suitable, the staining effect is good, the cells are evenly dispersed, can be counted, and can be used for karyotype analysis. After counting, the chromosome 2n=10 of Chinese peony cultivars.
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