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A method for improving the antioxidant capacity of dendrobium polysaccharides

A technology of anti-oxidation ability and Dendrobium officinale polysaccharide, which is applied in the field of improving the function of plant active ingredients, can solve the problems of unfavorable organisms absorbed into the body, large molecular weight of polysaccharides, poor water solubility, etc., to achieve improved medicinal value, simple operation, low cost effect

Active Publication Date: 2020-12-15
SOUTH CHINA AGRI UNIV +2
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Usually, in the directly extracted polysaccharide extract, the polysaccharide has a large molecular weight and poor water solubility, which is not conducive to the absorption of organisms into the body and exerts its biological activity. This limits the application of polysaccharides to a certain extent. A certain degree of degradation can It is of great significance to maintain or even improve the biological activity of polysaccharides

Method used

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  • A method for improving the antioxidant capacity of dendrobium polysaccharides
  • A method for improving the antioxidant capacity of dendrobium polysaccharides

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Embodiment 1

[0026] Agar plate medium: agar powder (dry powder) 20g / L, beef extract 3g / L, peptone 10g / L, NaCl 5g / L, pH value is 7.4±0.2; liquid medium: beef extract 3g / L, peptone 10g / L L, NaCl 5g / L, pH value is 7.4±0.2. The microorganism used is Bacillus subtilis, which is purchased from China General Microorganism Culture Collection and Management Center and is numbered 1.821 Bacillus subtilis, and is fermented according to the following steps:

[0027] (1) Extraction of polysaccharides from Dendrobium officinale by hot water extraction: Take 100g of fresh Dendrobium, cut into slices, and place them in a dryer at 105°C for drying treatment. After 5 minutes, dry them at 65°C to constant weight (about 41.31g), and grind them. 40-mesh sieve, add 20g of Dendrobium dry powder and 1600mL of distilled water according to the ratio of material to liquid of 1:80 (g:mL), and filter after heating in a water bath at 80°C for 1 hour. Water and ethanol until the final concentration of alcohol is 80%, o...

Embodiment 2

[0034] Agar plate medium: agar powder (dry powder) 20g / L, beef extract 3g / L, peptone 10g / L, NaCl 5g / L, pH value is 7.4±0.2; liquid medium: beef extract 3g / L, peptone 10g / L L, NaCl 5g / L, pH value is 7.4±0.2. The microorganism used is Bacillus subtilis, which is purchased from China General Microorganism Culture Collection and Management Center and is numbered 1.821 Bacillus subtilis, and is fermented according to the following steps:

[0035] (1) Extract polysaccharides from Dendrobium officinale by hot water extraction: Take 200g of fresh Dendrobium, cut into slices, bake at 105°C for 5 minutes, and dry at 65°C until constant weight (about 50.34g), grind through a 40-mesh sieve, and press 1:80( g:mL) Ratio of solid to liquid Add 30g of Dendrobium dry powder and 2400mL of distilled water, heat in a water bath at 90°C for 1 hour, then filter, repeat the extraction of the filter residue once, combine the two extracts, about 4.60L, add absolute ethanol until the final concentratio...

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Abstract

The invention discloses a microbial fermentation method for improving an antioxidant ability of dendrobium polysaccharides, and belongs to the field of functional improvement of plant active ingredients. According to the method, beneficial microorganisms are inoculated, microbial fermentation is utilized to degrade polysaccharides with the large molecular weight into micromolecular polysaccharides, and it is verified that the method effectively improves the oxidation resistance of the dendrobium polysaccharides by measuring the total antioxidant ability of a fermentation liquor and stock solution. The method has the advantages that the process is simple, the operation is convenient, the reaction conditions are mild, the method is friendly to the environment, and the implementation and promotion are easy; the molecular weight of the dendrobium polysaccharides obtained through the method is small, the dendrobium polysaccharides are conductive to being absorbed by organisms, the antioxidant ability is strong, and the method has a wide application prospect in the fields of functional drugs, food development and the like.

Description

technical field [0001] The invention relates to the field of function improvement of plant active ingredients, in particular to a method for improving the antioxidant capacity of dendrobium polysaccharides. Background technique [0002] Dendrobium (Dendrobium) is one of the earliest orchid plants recorded in ancient Chinese literature. It has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing heat, and its main active ingredient is polysaccharide. In the past ten years, relevant research work has mainly focused on the extraction methods, chemical components, pharmacological effects and fingerprints of Dendrobium officinale (Yao Chen, Research progress on polysaccharides from Dendrobium officinale, Chinese National Folk Medicine, 2015, 24 (15 ): 6-7). [0003] Polysaccharide refers to a polymer composed of more than 10 monosaccharides linked by glycosidic bonds. It is widely found in animals, plants and microbial tissues. It is one of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/125
CPCC12P19/04
Inventor 白文艳陈洁梅黄九九刘伟古志平吴保林乔晓颖陈丽花
Owner SOUTH CHINA AGRI UNIV
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