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Amplification primer for identifying green sea turtle and hawksbill products and its application and identification method

A technique for amplifying primers and turtles, which is applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc. It can solve problems affecting results and achieve simple operation, strong specificity, and short time-consuming Effect

Active Publication Date: 2022-06-28
海南通识科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many aspects such as species sex and developmental stage, cryptic species, phenotypic plasticity and genetic diversity will affect the results of traditional morphological identification methods

Method used

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  • Amplification primer for identifying green sea turtle and hawksbill products and its application and identification method
  • Amplification primer for identifying green sea turtle and hawksbill products and its application and identification method
  • Amplification primer for identifying green sea turtle and hawksbill products and its application and identification method

Examples

Experimental program
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Embodiment 1

[0031] Example 1: Comparison of two amplification primers

[0032] In the present invention, PCR amplification and sequencing is carried out on two kinds of sea turtles in preliminary morphological classification according to the universal primers of the COX1 DNA gene fragment of sea turtles, and edited by Seqman, artificially adjusted and optimized, and confirmed as two kinds of sea turtles in GenBank through BLAST homology comparison in GenBank 2 pairs of PCR primers including the primers of the present invention were manually designed using clustalx software:

[0033] Amplification primers of the present invention: shown in SEQ ID NOs: 1-2;

[0034] Comparative amplification primers: shown in SEQ ID NOs: 5-6;

[0035] The above two pairs of amplification primers were manually designed by the clustalx software under the condition that the target fragments are consistent and all meet the primer design rules;

[0036] DNA extraction (according to the instruction manual of Ge...

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PUM

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Abstract

The invention relates to the technical field of species molecular identification, and discloses an amplification primer for identifying green sea turtles and hawksbill products, and an application and identification method thereof. The amplification primer of the present invention has the sequence shown in SEQ ID NO: 1‑2. The amplification primer and method of the present invention are used to specifically identify green sea turtles (C.mydas) and hawksbill turtles (E.imbricata). The morphologically identified species of sea turtles provide technical support for the identification of sea turtles, and provide technical support for species identification to combat illegal trade in sea turtles (sea turtle handicrafts, illegal products).

Description

technical field [0001] The invention relates to the technical field of molecular identification of species, in particular to an amplification primer for identification of green sea turtles and hawksbill products and an application and identification method thereof. Background technique [0002] DNA barcoding is a standard, sufficiently variable and easily amplified DNA fragment that can represent the species in the organism. DNA barcoding technology is a simple mechanical repetition, which can be used quickly after simple training, and will make up for the inaccuracy of species identification caused by the convergence and variation of morphological identification features. DNA barcoding has made great contributions to the discovery of new species or cryptic species, and to the identification of endangered species, can make a huge contribution to the protection of biodiversity. [0003] There are only 2 families of sea turtles in the world (sea turtles and leatherbacks), 6 g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2563/185
Inventor 杜宇姚运涛刘其则陈忠荫赵一帆张子璇林炽贤计翔
Owner 海南通识科技有限公司