Liver model and its preparation method and application

Abstract

The invention relates to a liver model and its preparation method and application. The preparation method of the liver model comprises the following steps: decellularizing the liver to obtain a decellularized liver; and reinforcing the decellularized liver with a cross-linking agent , to obtain the above liver model. The preparation method of the liver model takes a short time, and the arrangement and mechanical properties of the vascular system of the prepared liver model are close to real liver cells, and the three-dimensional structure of the vascular system in the liver is preserved, and the whole is transparent, and the internal blood vessels are clearly visible, without the need for Borrow digital subtraction angiography and other equipment to directly observe the process of embolization, the distribution of embolism agent and embolization effect, which can be stored for a long time.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a liver model and its preparation method and application. Background technique [0002] Transcatheter arterial chemoembolization (Transarterial Chemoembolization, TACE) is currently one of the most commonly used interventional methods for the treatment of hepatocellular carcinoma. The embolic agents used in transcatheter arterial chemoembolization are commonly used conventional medical devices, such as lipiodol, gelatin sponge particles, and polyvinyl alcohol microspheres. At present, the following two models are commonly used to study the embolic effect of embolic agents: [0003] (1) Microfluidic in vitro model, which simulates the multi-level blood vessel distribution of the liver based on a microfluidic chip, and injects embolic agent into it to observe and evaluate its embolic effect. For example, two or more microfluidic chips are connected in parallel to form a microvas...

AI Technical Summary

Problems solved by technology

First of all, for in vitro microfluidic models, there is still a big difference between using a microfluidic chip to simulate blood vessels at all levels of the liver and the actual liver vascular system, and the microfluidic chip is often made of artificial polymers such as polydimethylsiloxane. There are certain differences between the mechanical properties of the material and the actual blood vessel

Secondly, if animals are used to evaluate the embolic effect of embolic agents, it will take a lot of money and time to perform embolization surgery and postoperative animal feeding and care, and this process requires a large number of expensive supporting facilities and the participation of many professionals

In addition, the results of in vivo experiments are not intuitive, and expensive instruments such as MRI, digital subtraction angiography, etc. are required to observe and evaluate the effect of embolism, or take out the liver after a long period of time (such as two weeks) to observe and evaluate embolism Effect

In addition, most of the current microparticle or microsphere embolism agents do not have the ability to develop, and intraoperative and postoperative evaluation requires additional injection of contrast agents for imaging observation, which increases the difficulty and cost of surgical operations

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