The invention discloses a sterile soft package isolator which comprises an outer frame support. An air feeding and discharging box and a transparent PVC (polyvinyl chloride) flexible film isolation package, an operating glove and a delivery bin are arranged on the transparent PVC flexible film isolation package and connected with the air feeding and discharging box through an air feeding pipe andan air discharging pipe, pipeline-type efficient filters and manual adjusting valves are arranged on pipe bodies of the air feeding pipe and the air discharging pipe, the air feeding and discharging box comprises an air feeding primary filter, an air feeding fan, an air feeding efficient filter, a concentrated air feeding chamber, a concentrated air discharging chamber, an air discharging fan, anexternal air discharging connector, an all-network wireless communication module, a measurement sensor, an integrated control module, a UPS (uninterrupted power supply) and the like. The sterile softpackage isolator is conveniently and rapidly assembled and disassembled, various operations of the workers are facilitated, pollution risks are low, collapse cannot easily generated, manufacturing andtransporting cost is low, the sterile soft package isolator has intelligent control and remote control functions, and healthy living environments can be created for sterile animals.