Liver model and preparation method and application thereof

A technology of liver and model, applied in the field of liver model and its preparation, can solve problems such as differences in vascular mechanical properties, increased difficulty and cost of surgical operations, and unintuitive results

Active Publication Date: 2019-04-23
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, for in vitro microfluidic models, there is still a big difference between using a microfluidic chip to simulate blood vessels at all levels of the liver and the actual liver vascular system, and the microfluidic chip is often made of artificial polymers such as polydimethylsiloxane. There are certain differences between the mechanical properties of the material and the actual blood vessel
Secondly, if animals are used to evaluate the embolic effect of embolic agents, it will take a lot of money and time to perform embolization surgery and postoperative animal feeding and care, and this process requires a large number of expensive supporting facilities and the participation of many professionals
In addition, the results of in vivo experiments are not intuitive, and expensive instruments such as MRI, digital subtraction angiography, etc. are required to observe and evaluate the effect of embolism, or take out the liver after a long period of time (such as two weeks) to observe and evaluate embolism Effect
In addition, most of the current microparticle or microsphere embolism agents do not have the ability to develop, and intraoperative and postoperative evaluation requires additional injection of contrast agents for imaging observation, which increases the difficulty and cost of surgical operations

Method used

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  • Liver model and preparation method and application thereof
  • Liver model and preparation method and application thereof
  • Liver model and preparation method and application thereof

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preparation example Construction

[0031] The preparation method of the liver model of one embodiment, comprises the following steps:

[0032] S110. Freezing the isolated liver.

[0033] The liver used in this embodiment refers to an isolated liver. The isolated liver can be the liver of animals such as mice, rats, rabbits, pigs, monkeys, dogs and sheep with diseased or damaged livers. For example, livers carrying tumors, further such as livers of New Zealand rabbits carrying VX2 tumors, or cadaveric livers of liver cancer patients obtained according to corresponding national laws and ethical principles, etc. The isolated liver can also be the healthy liver of animals such as mice, rats, rabbits, pigs, monkeys, dogs and sheep. In this embodiment, the liver used refers to the whole liver. Of course, it can be understood that in some other embodiments, a part of the liver can be used according to actual needs.

[0034] Specifically, the freezing temperature is -70°C to -80°C, and the freezing time is more tha...

Embodiment 1～5

[0074] (1) According to Table 1, the livers of each embodiment and blank control (untreated liver) are provided, wherein, the livers of Examples 1-6 and blank control come from the Guangzhou Provincial Experimental Animal Center, and the livers of Examples 1-6 are from the Guangzhou Provincial Experimental Animal Center. 6 and the blank control have the same biological size of the liver. Wherein, the fresh liver of embodiment 6 such as figure 1 shown. The livers of each embodiment and blank control (untreated liver) were frozen for 24 hours. Wherein the liver after freezing 24h of embodiment 6 is as follows figure 2 shown.

[0075] Table 1

[0076]

[0077] (2) The hepatic artery and portal vein of the liver of each embodiment were inserted and fixed with needles, and the portal vein needles were sealed for later auxiliary tests. Inject different washing solutions into the hepatic portal vein as needed to clean the liver step by step. During the cleaning process, air ...

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Abstract

The invention relates to a liver model and a preparation method and application thereof. The preparation method of the liver model comprises the following steps that the liver is decellularized, and the decellularized liver is obtained; the decellularized liver is reinforced with a cross-linking agent, and the liver model is obtained. The preparation method of the liver model is short in time, theblood vascular system distribution and the mechanical property of the liver model prepared by using the method are close to real liver cells, the three-dimensional structure of a blood vascular system in the liver is reserved, the overall liver model is transparent, and the blood vessels in the liver model are clearly visible; the embolization process, the distribution of an embolic agent and theembolization effect can be directly observed without a digital subtraction angiography instrument or other devices, and the storage time is long.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a liver model and its preparation method and application. Background technique [0002] Transcatheter arterial chemoembolization (Transarterial Chemoembolization, TACE) is currently one of the most commonly used interventional methods for the treatment of hepatocellular carcinoma. The embolic agents used in transcatheter arterial chemoembolization are commonly used conventional medical devices, such as lipiodol, gelatin sponge particles, and polyvinyl alcohol microspheres. At present, the following two models are commonly used to study the embolic effect of embolic agents: [0003] (1) Microfluidic in vitro model, which simulates the multi-level blood vessel distribution of the liver based on a microfluidic chip, and injects embolic agent into it to observe and evaluate its embolic effect. For example, two or more microfluidic chips are connected in parallel to form a microvas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/28G09B23/30
CPCG09B23/28G09B23/306
Inventor 郭琼玉高雅楠陈梓健
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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