Method for direct regeneration of hypertrophic cartilage tissue using adipose tissue and scaffold material

A technology of adipose tissue and scaffold materials, applied in tissue regeneration, bone/connective tissue cells, prostheses, etc., can solve the problem of weakened multidirectional differentiation ability of mesenchymal stem cells, achieve multidirectional differentiation and angiogenesis potential, Effect of promoting osteoclast chemotaxis and promoting bone regeneration

Active Publication Date: 2021-06-04
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] The present invention proposes a method for directly regenerating hypertrophic cartilage tissue using adipose tissue and scaffolding materials, which solves the problems in the prior art that the multidirectional differentiation ability of ASC as mesenchymal stem cells is weakened after a long period of passage in vitro

Method used

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  • Method for direct regeneration of hypertrophic cartilage tissue using adipose tissue and scaffold material
  • Method for direct regeneration of hypertrophic cartilage tissue using adipose tissue and scaffold material
  • Method for direct regeneration of hypertrophic cartilage tissue using adipose tissue and scaffold material

Examples

Experimental program
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Embodiment 1

[0067] A method for directly regenerating hypertrophic cartilage tissue using adipose tissue and scaffold material, comprising the following steps (see figure 1 ):

[0068] (1) Preparation of microparticle adipose tissue

[0069] (a) Human adipose tissue obtained from liposuction operation, after repeated rinsing with saline, the upper layer of adipose tissue was collected;

[0070] (b) Cut the upper layer of adipose tissue with scissors, centrifuge at 1200g for 5 minutes, remove the upper layer of fat and lower layer of swelling fluid, and collect the middle fat layer in a 20mL syringe;

[0071] (c) Use a three-way tube to connect two 20mL syringes, inject the two syringes back and forth 30 times, and obtain particulate adipose tissue;

[0072] (d) The particulate adipose tissue was centrifuged at 1500 g for 5 minutes to remove the upper layer of fat layer, and the lower layer was purified particulate adipose tissue.

[0073] (e) Add HA / β-TCP scaffold material to micro-adi...

Embodiment 2

[0095] The difference between this example and Example 1 is that the volume of the HA / β-TCP scaffold material and the particulate adipose tissue is 1:8, and other steps are the same as Example 1.

Embodiment 3

[0097] The difference between this example and Example 1 is that the volume of the HA / β-TCP scaffold material and the particulate adipose tissue is 1:16, and other steps are the same as Example 1.

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Abstract

The present invention proposes a method for directly regenerating hypertrophic cartilage tissue using adipose tissue and a scaffold material, comprising: (1) adding a scaffold material to particulate adipose tissue; (2) proliferating and culturing in vitro to form mesenchymal stem cells rich in fat (ASC) and still have multi-directional differentiation potential composite tissue block; (3) in vitro construction of hypertrophic cartilage tissue. In the present invention, a support material is added, and the added support material has the following effects: (1) provides an attachment surface for the cells in the adipose tissue, can promote the proliferation and migration of ASC, and increase the number of ASC; (2) promote hypertrophy Mineralization of cartilage tissue, thereby promoting bone regeneration; (3) promoting osteoclast chemotaxis, thereby promoting bone remodeling and bone marrow regeneration; (4) as a substitute for bone transplantation, increasing clinical maneuverability.

Description

technical field [0001] The invention relates to the technical field of bone tissue regeneration, in particular to a method for directly regenerating hypertrophic cartilage tissue using adipose tissue and scaffold materials. Background technique [0002] The traditional bone tissue engineering technology is to plant stem cells on biomaterials. After a period of in vitro culture, stem cells differentiate into bone cells on the biomaterials, secrete collagen, and finally regenerate new bone tissue, and then transplanted in bone defects. Repair function and appearance defects. At present, there are two major challenges in applying the bone tissue engineering technology in the laboratory to the clinic. On the one hand, it is difficult to obtain stem cells that are easy to obtain, have less damage, and have strong bone regeneration ability. At present, stem cell-based tissue engineering technology mainly obtains a sufficient number of stem cells from limited clinical tissue sampl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077A61L27/36A61L27/38A61L27/20A61L27/12
CPCA61L27/12A61L27/20A61L27/3604A61L27/365A61L27/3654A61L27/3687A61L27/3691A61L27/3834A61L27/3847A61L27/3852A61L2430/02A61L2430/06C08L5/08
Inventor 黄如林李青峰王文进李佳奇傅娆
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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