Efficient corn growth promoting bacterium capable of reducing application of nitrogen phosphorus fertilizer and application of efficient corn growth-promoting bacterium

A technology of growth-promoting bacteria and microbial strains, applied in the field of microorganisms, can solve the problems of high cost of biological fertilizers, achieve the effect of reducing nitrogen fertilizer input, reducing pollution, and promoting green, healthy and sustainable development

Active Publication Date: 2019-04-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many varieties of microbial fertilizers in our country, the main development and application targets are plants with high economic value such as fruits and vegetables. There are very few suitable for food crops, and even fewer suitable for corn. In addition, the cost of existing biological fertilizers is high. , in fact, farmers can't afford it in corn planting

Method used

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  • Efficient corn growth promoting bacterium capable of reducing application of nitrogen phosphorus fertilizer and application of efficient corn growth-promoting bacterium
  • Efficient corn growth promoting bacterium capable of reducing application of nitrogen phosphorus fertilizer and application of efficient corn growth-promoting bacterium
  • Efficient corn growth promoting bacterium capable of reducing application of nitrogen phosphorus fertilizer and application of efficient corn growth-promoting bacterium

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Experimental program
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Effect test

Embodiment 1

[0058] Example 1, Bacillus MGPR1 isolation and identification

[0059] Corn plays a vital role in my country's agricultural production and development. With the improvement of material level and science and technology, corn no longer only plays the role of food raw material. are increasingly used. Northeast my country is one of the main corn-growing areas in China, and the black soil in Northeast China urgently needs to take effective measures to protect it to curb the degradation of black soil. The present invention aims at corn production and soil status in Northeast China, by isolating rhizosphere bacteria from corn rhizosphere soil and measuring the growth-promoting functions of the strains, such as nitrogen fixation, phosphorus dissolution, potassium dissolution, IAA production, siderophore production, stress resistance, anti disease, etc., selected high-efficiency strains, and applied them to the long-term positioning test field of nitrogen- and phosphorus-deficient soil ...

Embodiment 2

[0142] Embodiment 2, field application of bacillus MGPR1

[0143] 1. Field application method of Bacillus MGPR1

[0144] 1. Strain activation and expansion culture: Bacillus sp. MGPR1CGMCC No.17040 strains stored in a -80°C refrigerator were activated in LB solid medium by three-section line method, and cultured in a 37°C constant temperature incubator After 24 hours, single colonies were isolated. Pick a single colony with a sterile bamboo stick to LB liquid medium supplemented with tryptophan (0.1mg / ml, function: the substrate for the synthesis of IAA) for expansion, and place it on a shaker at 37°C and 180 rpm Cultivate to bacterial solution OD 600 = 1.5 (with no culture medium as blank control, the concentration of bacteria solution is 1 × 10 10-11 cfu / ml);

[0145] 2. Before use, add trace elements (1μL / ml, effect: increase nutrition and improve bacterial activity) to the bacterial agent and mix well. The composition of the trace element solution is as follows: H 3 ...

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Abstract

The invention discloses an efficient corn growth promoting bacterium capable of reducing application of nitrogen phosphorus fertilizer and application of the efficient corn growth-promoting bacterium.The growth-promoting bacterium provided by the invention is a Bacillus sp. MGPR1, the preservation No. of the Bacillus sp. MGPR1 is CGMCC No.17040 in the CGMCC (China general microbiological culturecollection center). The Bacillus sp. MGPR1 can be used for promoting the height of a corn plant, the overground and underground biomass, the chlorophyll and the leaf area index to be obviously increased at different stages, the yield is obviously increased by 12.56% (nitrogen is reduced by 50%) and 7.73% (phosphorusis reduced by 100%) compared with the yield of nitrogen-deficient contrast corns and the yield of phosphorus-deficient contrast corns, and the yield has no obvious difference with that after normal fertilizer application. The Bacillus sp.MGPR1 is a stable and efficient fertilizer-reduction and efficiency-increase microbial fertilizer bacterium resource.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a corn high-efficiency growth-promoting bacterium capable of reducing the application of nitrogen and phosphorus fertilizers and its application. Background technique [0002] In my country's agricultural production, the amount of fertilizer application is large, but the utilization rate is not high and the loss is serious. The long-term excessive application of chemical fertilizers has also caused certain damage to the soil, such as soil compaction, acidification, salinization, and a series of problems such as the destruction of microbial community structure (reduction of beneficial microorganisms and increase of harmful microorganisms), which has also caused food safety. a certain threat. So how to effectively solve these agricultural production obstacles in our country and realize the sustainable development of agriculture? This coincides with the function of microbial fertiliz...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05F11/08A01C1/06C12R1/07
CPCA01C1/06A01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07Y02P60/21
Inventor隋新华陈腊米国华
OwnerCHINA AGRI UNIV