Seedling culturing method for purple sweet potatoes
A purple potato and seedling technology, which is applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc. Problems such as breeding and planting, to achieve the effects of accelerating early differentiation and proliferation, accelerating differentiation and proliferation, strengthening glycolysis process and mitochondrial activity
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Embodiment 1
[0038] A kind of seedling raising method of purple sweet potato, it specifically comprises the following steps:
[0039] 1 Sterilization: Collect the young budding stems of purple sweet potato from the field, remove the leaves and excess petioles, trim them into 0.5cm-long stems, wash off the dust and soil on the surface with a brush under running water, and then use Sterilize with ascorbic acid solution, wash with washing powder solution, rinse with running water for 30 minutes; sterilize with alcohol, rinse with sterile water twice, then sterilize with mercury chloride solution, rinse with sterile water twice for later use; Bacteria can kill the harmful microorganisms carried by the shoot tip of purple potato, avoid introducing harmful microorganisms into the culture medium to cause pollution, and at the same time prevent the absorption and utilization of the culture medium by microorganisms, and provide sufficient nutrients for the shoot tip cells for their differentiation; ...
Embodiment 2
[0053] A method for raising seedlings of purple sweet potato, comprising:
[0054] a) Get the young and tender stem section of purple potato with buds and trim it into small sections, then carry out sterilization treatment;
[0055] b) the subsection of step a) is propagated and cultivated in a proliferation medium containing powdery activated carbon to obtain seedlings;
[0056] c) the seedlings of step b) are cultivated in the rooting medium to obtain the increased seedlings of purple sweet potato;
[0057] d) The purple sweet potato seedlings are sterilized after hardening and transplanted.
[0058] Wherein, the proliferation medium is MS medium containing 8g / L sucrose, 15mg / L benzylquinine chloride, 0.4g / L ethanol, and 0.12g / L oxytetracycline.
[0059] In the method of the present invention, firstly, the young purple sweet potato stem section with buds collected from the field is trimmed, and after being sterilized with ascorbic acid solution, alcohol, and mercuric chlorid...
Embodiment 3
[0076] Example 3 is basically the same as Example 2, except that in Example 3, the proliferation medium does not contain benzylquinine chloride.
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