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A triple-internal standard based glycan structural assignment method for capillary electrophoresis analysis of carbohydrates

An internal standard, glycan technology, applied in the field of triple internal standard-based glycan structure assignment for carbohydrate capillary electrophoresis analysis

Active Publication Date: 2019-05-24
DH TECH DEVMENT PTE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0019] Additionally, traditional LC- or CE-based GU experiments require comparisons between separate experiments performed on the same device

Method used

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  • A triple-internal standard based glycan structural assignment method for capillary electrophoresis analysis of carbohydrates
  • A triple-internal standard based glycan structural assignment method for capillary electrophoresis analysis of carbohydrates
  • A triple-internal standard based glycan structural assignment method for capillary electrophoresis analysis of carbohydrates

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Embodiment Construction

[0035] computer implemented system

[0036] figure 1 To illustrate a block diagram of a computer system 100 on which embodiments of the present teachings may be implemented. Computer system 100 includes a bus 102 or other communication mechanism for communicating information, and a processor 104 coupled with bus 102 for processing information. Computer system 100 also includes memory 106 , which may be a random access memory (RAM) or other dynamic storage device coupled to bus 102 for storing instructions to be executed by processor 104 . Memory 106 may also be used to store temporary variables or other intermediate information during execution of instructions executed by processor 104 . Computer system 100 further includes a read only memory (ROM) 108 or other static storage device coupled to bus 102 for storing static information and instructions for processor 104 . A storage device 110, such as a magnetic or optical disk, is provided and coupled to bus 102 for storing...

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Abstract

The present invention provides a separation device receiving a known or unknown glycan that is co-injected with three different oligomers maltooligosaccharide (MOL). A detector measures the separatedglycan and the separated three different oligomers as intensity peaks that are a function of migration time. The migration times of a plurality of other oligomers of MOL are calculated from the migration times of the three different oligomers. Glucose unit (GU) values for the intensity peaks of the separated glycan are calculated by comparing their migration times to the calculated migration timesof the plurality of other oligomers of MOL. The processor identifies the structure of the glycan by comparing the calculated GU values of the intensity peaks of the separated glycan to a database ofGU values for known glycan structures.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 380,062, filed August 26, 2016, and U.S. Provisional Patent Application No. 62 / 518,104, filed June 12, 2017, both of which The contents of the two U.S. Provisional Patent Applications are incorporated herein by reference in their entirety. Background technique [0003] The teachings herein relate to identifying the structure of carbohydrates or any glycans in a sample using separation techniques such as capillary electrophoresis or liquid chromatography. More particularly, the teachings herein relate to systems and methods for identifying the structure of glycans using co-injection of triple internal standards for glucose unit (GU) calculations that alleviate the concomitantity of such calculations on the maltooligosaccharide ladder running needs. The demarcation standard was co-injection and the internal standard was the fraction of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/487G01N27/447G01N21/33G01N30/88
CPCG01N21/6402G01N27/44726G01N2030/045G01N2030/8836G01N21/33G01N33/5308G16C20/20
Inventor G·贾尔瓦斯M·G·西盖蒂A·格特曼J·查普曼
Owner DH TECH DEVMENT PTE