A bottom-illuminated nucleic acid isothermal amplification detection portable instrument

Abstract

The invention discloses a bottom-illuminated nucleic acid isothermal amplification detection portable instrument. It includes a fluorescence detector, a reagent storage area, a pipette gun storage box, a consumable storage box, and a battery box. The above five parts are placed in a suitcase; the reagent storage area is composed of a reagent storage box and a reagent storage box cover. There are multiple centrifuge tube holes in the box, the top of the reagent storage box has a water injection port for the reagent storage box, and the water level limit hole for the reagent storage box is opened on the side wall, and water is used as the freezing medium; the fluorescence detector includes an instrument shell and a sample rack module , control circuit module, fluorescence detection module, heating module and host computer module. The present invention aims at the real-time quantitative detection of biochemical samples by the fluorescence analysis method based on nucleic acid isothermal amplification technology, realizes the integration and high throughput of amplification and detection, requires less samples to be tested, the whole system is small in size and easy to carry, and has high measurement accuracy. The detection speed is fast, and it is suitable for on-site rapid detection of food safety.

Description

technical field [0001] The invention relates to the field of biochemical sample detection instruments in food safety, in particular to a bottom-illuminated nucleic acid isothermal amplification detection portable instrument, which combines nucleic acid isothermal amplification technology with mechanical and electronic control to form an automatic detection device system. Background technique [0002] As one of the most commonly used molecular biology techniques, polymerase chain reaction (PCR) can amplify a small amount of target DNA by millions of times. It has the advantages of strong specificity, high sensitivity, and simplicity, so it is widely used Applied in the field of food safety testing. However, conventional PCR technology has the following disadvantages: the instrument is expensive, bulky and difficult to carry, not suitable for on-site detection, and easily causes non-specific amplification. [0003] The newly developed nucleic acid isothermal amplification tec...

AI Technical Summary

Problems solved by technology

However, the conventional PCR technology has the following disadvantages: the instrument is expensive, bulky and difficult to carry, not suitable for on-site detection, easy to cause non-specific amplification, etc.

[0006] Existing fluorescence detectors often use xenon lamps, deuterium lamps, mercury lamps, light-emitting diodes (LEDs), laser diodes, etc. Although LEDs and laser diodes are small in size and easy to integrate, they have a single light-emitting band that is difficult to meet the excitation requirements of various fluorescent substances, and the current LED-based fluorescence detector has a fixed luminous power that cannot be adjusted, making it difficult to meet the excitation requirements of different concentrations of analytes.

At the same time, the excitation light source of most detectors is located on the top of the sample container, and the light intensity irradiated on the reagent at the bottom of the sample container is greatly reduced due to the large optical path and the scattering of the container.

The receiver of a fluorescence detector often uses a photomultiplier tube (Photomultiplier, PMT). The conventional PMT has the advantages of sensitive detection and fast response, but its large size is not suitable for multi-channel and portable detection. Although there are miniaturized PMTs, But the price is expensive, which greatly increases the cost of the instrument

In addition, most of the current multi-channel fluorescence detectors use the same reference to eliminate the noise of different channels, but the noise of different channels will be slightly different due to the different sensitivity of the detection instrument, detection position, signal processing circuit, etc. The same reference is not conducive to multiple channels. Consistency of channel detection

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