A top-illuminated nucleic acid isothermal amplification detection portable instrument

Abstract

The invention discloses a portable top-lit nucleic acid isothermal amplification detection instrument, which comprises a fluorescence detector, a reagent storage area, a pipette storage box, a consumable storage box and a battery box. The fluorescence detector, the reagent storage area, the pipette storage box, the consumable storage box and the battery box are placed in a suitcase. The reagent storage area is formed by connection of a reagent storage box and a reagent storage box cover, the reagent storage box is internally provided with a plurality of centrifugal tube holes, the top end of the reagent storage box is provided with a reagent storage box water injection port, the side wall is provided with a reagent storage box water level limiting hole, and water serves as a refrigerationmedium. The fluorescence detector comprises an instrument outer shell and sample holder module, a control circuit module, a fluorescence detection module, a heating module and an upper computer module. By the nucleic acid isothermal amplification technology based fluorescence analysis method for quantitative detection of biochemical samples, amplification and detection integration and high flux are realized, a low quantity of to-be-detected samples are needed, and a whole system is small in size, portable, high in measurement accuracy, quick in detection and suitable for onsite quick detectionfor food safety.

Description

technical field [0001] The invention relates to an isothermal amplification detector in the field of biochemical sample detection instruments in food safety, in particular to a top-illuminated nucleic acid isothermal amplification detection portable instrument, which combines nucleic acid isothermal amplification technology with mechanical and electronic control to form a set Automatic detection device system. Background technique [0002] As one of the most commonly used molecular biology techniques, polymerase chain reaction (PCR) can amplify a small amount of target DNA by millions of times. It has the advantages of strong specificity, high sensitivity, and simplicity, so it is widely used Applied in the field of food safety testing. However, conventional PCR technology has the following disadvantages: the instrument is expensive, bulky and difficult to carry, not suitable for on-site detection, and easily causes non-specific amplification. [0003] The newly developed ...

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Problems solved by technology

However, the conventional PCR technology has the following disadvantages: the instrument is expensive, bulky and difficult to carry, not suitable for on-site detection, easy to cause non-specific amplification, etc.

[0006] Existing fluorescence detectors often use xenon lamps, deuterium lamps, mercury lamps, light-emitting diodes (LEDs), laser diodes, etc. Although LEDs and laser diodes are small in size and easy to integrate, they have a single light-emitting band that is difficult to meet the excitation requirements of various fluorescent substances, and the current LED-based fluorescence detector has a fixed luminous power that cannot be adjusted, making it difficult to meet the excitation requirements of different concentrations of analytes.

The receiver of a fluorescence detector often uses a photomultiplier tube (Photomultiplier, PMT). The conventional PMT has the advantages of sensitive detection and fast response, but its large size is not suitable for multi-channel and portable detection. Although there are miniaturized PMTs, But the price is expensive, which greatly increases the cost of the instrument

In addition, most of the current multi-channel fluorescence detectors use the same reference to eliminate the noise of different channels, but the noise of different channels will be slightly different due to the different sensitivity of the detection instrument, detection position, signal processing circuit, etc. The same reference is not conducive to multiple channels. Consistency of channel detection

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