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Method for establishing chicken embryo fibroblast monoclonal cell line

A fibroblast, monoclonal technology, applied in embryonic cells, microorganism-based methods, animal cells, etc., can solve the problems of high price, low efficiency, narrow application range, etc., and achieve low price, simple operation and easy purchase of finished products. Effect

Pending Publication Date: 2019-06-04
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the shortcomings of the current method for preparing single cell clones: low efficiency, high price, narrow application range, etc., the present invention aims to overcome the above shortcomings and invent a simple, cheap and efficient monoclonal cell line of chicken embryo fibroblasts established method

Method used

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  • Method for establishing chicken embryo fibroblast monoclonal cell line
  • Method for establishing chicken embryo fibroblast monoclonal cell line
  • Method for establishing chicken embryo fibroblast monoclonal cell line

Examples

Experimental program
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Effect test

Embodiment Construction

[0020] Such as figure 1 As shown, connect the 1ml syringe 1 (remove the plunger and needle tip, keep the syringe), 0.22 micron syringe filter 3, 2.5 micron inner diameter, 10 cm long rubber tube 4, the BJ40 glass tube 5 (needle tube) drawn to form shaped glass tube).

[0021] The specific operation is as follows:

[0022] Part 1: Discard the plunger and needle tip of the 1ml syringe, and insert a clean and dry cotton ball 2 into the syringe tube for the purpose of preliminarily filtering the impurities in the port.

[0023] The second part: connect the big end of the 0.22 micron syringe filter to the needle port of the 1 ml syringe, the connection should be well sealed, the purpose is to filter the cells and bacteria in the port.

[0024] The third part: connect one end of a rubber tube with an inner diameter of 2.5 microns and a length of 10 cm to the small head of a 0.22 micron syringe filter, and the connection should be well sealed.

[0025] The fourth part: First, pinc...

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Abstract

The invention relates to a method for establishing a chicken embryo fibroblast monoclonal cell line, and belongs to the technical field of biology. The method is as follows: cramming clean and dry cotton balls into an injection tube, connecting a big head of a needle tube-type filter with a needle opening of an injector, connecting one end of a rubber tube with a small head of the needle tube-typefilter, and connecting the other end of the rubber tube with a treated glass tube for directly cell picking. In the invention, the materials adopted by an oral siphon are easy to purchase and low inprice. After the assembly is finished, the finished product is simple to operate, a user can pick a specific single cell for monoclonal culture, and the adopted recombinant oral siphon is suitable forall types of cells.

Description

technical field [0001] The invention relates to a method for establishing a chicken embryo fibroblast monoclonal cell line, belonging to the field of biotechnology. Background technique [0002] Chicken embryonic fibroblasts are often used as the experimental object of transfection, challenge and other experiments in in vitro research, and are an important model cell for in vitro experiments. However, the primary isolated embryonic fibroblasts usually contain more miscellaneous cells, their culture and proliferation ability is not stable, and the cell uniformity is poor. Therefore, it is particularly important to establish a single-cell clone cell line of chicken embryo fibroblasts. [0003] Now the main methods for preparing single cell clones mainly include limiting dilution method, cloning ring method, mexisol or agar gel cloning method, flow cytometry and so on. However, the limited dilution method and cloning ring method are inefficient in picking monoclonals, and it t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073C12R1/91
Inventor 金晶赵瑞丰张晨李碧春张亚妮左其生孙红艳
Owner YANGZHOU UNIV