Method for extracting and separating active zinc polysaccharide from lycium chinense pollen

The technology of zinc polysaccharide and wolfberry is applied in the field of extraction and separation of active zinc polysaccharide, which can solve the problems of less research and development work on functional components and no extraction and separation method of active polysaccharide from wolfberry pollen, etc. Effects of immune function

Inactive Publication Date: 2019-06-07
WOLFBERRY ENG RES INST NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are few researches and developments on its functional components, especially the specific extraction and separation methods for active polysaccharides from wolfberry pollen have not yet been developed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: A method for extracting and isolating active zinc polysaccharides from wolfberry pollen. The method is to routinely pulverize wolfberry bee pollen, then use 5 times the volume of ether to extract at room temperature for 5 hours, filter to remove the organic layer, and then use 5 times the volume The deionized water extraction residue was extracted 3 times at 30-50°C for 2 hours each time. After the water extracts were combined and concentrated under reduced pressure, four times the volume of absolute ethanol was added to precipitate overnight at 4°C. Centrifuge at a speed of rpm for 15 minutes to collect the precipitate, dissolve it in 5-10 times the deionized solution, and dialyze it with a 5000 molecular weight dialysis bag for 48 hours. After dialysis, the extract is concentrated under reduced pressure and freeze-dried to obtain crude wolfberry pollen zinc polysaccharide, and then purified, eluted, concentrated, dialyzed, and freeze-dried to obtain the wolf...

Embodiment 2

[0020] Example 2: A method for extracting and isolating active zinc polysaccharides from wolfberry pollen. The method is to routinely pulverize wolfberry bee pollen, then use 6 times the volume of diethyl ether to extract at room temperature for 5 hours, filter to remove the organic layer, and then use 6 times the volume of The deionized water extraction residue was extracted 3 times at 30-50°C for 2 hours each time. After the water extracts were combined and concentrated under reduced pressure, four times the volume of absolute ethanol was added to precipitate overnight at 4°C. Centrifuge at a speed of rpm for 15 minutes to collect the precipitate, dissolve it in 5-10 times the deionized solution, and dialyze it with a 6000 molecular weight dialysis bag for 48 hours. After dialysis, the extract is concentrated under reduced pressure and freeze-dried to obtain crude wolfberry pollen zinc polysaccharide, and then purified, eluted, concentrated, dialyzed, and freeze-dried to obta...

Embodiment 3

[0022] Example 3: A method for extracting and isolating active zinc polysaccharides from wolfberry pollen. The method is to routinely pulverize wolfberry bee pollen, then use 6 times the volume of diethyl ether to extract at room temperature for 5 hours, filter to remove the organic layer, and then use 6 times the volume The deionized water extraction residue was extracted 3 times at 30-50°C for 2 hours each time. After the water extracts were combined and concentrated under reduced pressure, four times the volume of absolute ethanol was added to precipitate overnight at 4°C. Centrifuge at a speed of rpm for 15 minutes to collect the precipitate, dissolve it in 5-10 times the deionized solution, and dialyze it with a 6000 molecular weight dialysis bag for 48 hours. After dialysis, the extract is concentrated under reduced pressure and freeze-dried to obtain crude wolfberry pollen zinc polysaccharide, and then purified, eluted, concentrated, dialyzed, and freeze-dried to obtain ...

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PUM

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Abstract

The invention relates to an extraction separation method for active zinc polysaccharide, and in particular relates to a method for extracting and separating the active zinc polysaccharide from lyciumchinense pollen. The method comprises the following steps: crushing the lycium chinense bee pollen, performing extraction by using ether with a volume of 4-8 times at a room temperature for 3-6 h, performing filtration to remove an organic layer, performing extraction on residues by using deionized water with a volume of 3-6 times, wherein extraction is performed for three times at 30-50 DEG C, and each extraction is performed for 2 h, merging water extracts, performing reduced-pressure concentration, adding absolute ethanol with a volume of 3-6 times, performing precipitation at 4 DEG C overnight, performing centrifugation at 4000 rpm for 15 min to collect a precipitate, dissolving the precipitate into a deionized solution with a volume of 5-10 times, performing dialysis for 48 h by usinga dialysis bag with a molecular weight of 4000-8000, after dialysis is completed, performing reduced-pressure concentration on the extract, performing lyophilization to obtain crude lycium chinense pollen zinc polysaccharide, performing purification, performing elution, performing concentration, performing dialysis, and performing lyophilization to obtain the lycium chinense pollen zinc polysaccharide. The method provided by the invention is unique and has physiological functions and pharmacological action of enhancing Immunity, resisting aging, reducing blood fat and suppressing prostatic hyperplasia.

Description

technical field [0001] The invention relates to a method for extracting and separating active zinc polysaccharides, in particular to a method for extracting and separating active zinc polysaccharides from wolfberry pollen. Background technique [0002] Goji berry has a long history of health preservation, and now it is highly valued by experts and scholars. The "Compendium of Materia Medica" written by Li Shizhen, an outstanding medical scientist in the Ming Dynasty, records: "Godberry can nourish the kidney and generate essence, nourish the liver, improve eyesight, strengthen bones, relieve fatigue, change color, whiten, improve eyesight and calm the nerves, and make people live longer... …Flowers picked in summer are called 'Changshengcao'; fruits picked in autumn are called "'Goji'...". That is, besides goji fruit, goji flowers are also treasures. Modern research also shows that goji flowers are rich in phenolic acids, flavonoids, sugar beet Alkaline, and other active in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04A61P39/06A61P3/06A61P15/08A61P35/00
Inventor 闫亚美米佳冉林武曹有龙禄璐金波罗青张志娟李晓莺安巍石志刚张曦燕古玲玉
Owner WOLFBERRY ENG RES INST NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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