Device capable of performing real-time observation of bacterial motility track

Abstract

The invention belongs to the crossing fields of a semiconductor and a biological technology, and particularly relates to a device capable of performing real-time observation of a bacterial motility track. The device comprises a CMOS sensor, a bacterium carrier, a light source and an image processor, wherein the light source is arranged above the bacterium carrier; the light source consists of a first LED lamp and a second LED lamp which have different wave lengths, a light outlet of the first LED lamp vertically faces downwards, and emitted light rays are perpendicular to the plane of the bacterium carrier; a light outlet of the second LED lamp is obliquely arranged until the angle of 45 degrees is formed between the emitted light rays and the plane of the bacterium carrier; and a CMOS sensor chip is arranged in the CMOS sensor, and the CMOS sensor is arranged just below the bacterium carrier and is connected with the image processor. An optical system built with the high-resolution CMOS sensor is used for reconstructing the three-dimensional track of bacteria, and under different conditions, the three-dimensional track of the bacteria is observed in a high-flux real-time manner.

Description

technical field [0001] The invention belongs to the intersecting field of semiconductor and biotechnology, in particular to a device capable of observing the trajectory of bacteria in real time. Background technique [0002] Bacteria refer to a large group of primitive single-celled organisms with a nucleus without a nuclear membrane and only naked DNA called a nucleoid region. Bacteria in the narrow sense that people usually refer to are a type of prokaryotic microorganisms, which are short in shape, simple in structure, and mostly reproduce by binary fission. They are the most widely distributed organisms in nature and have the largest number of individuals. A major player in the material cycle of nature. At the same time, a large number of bacteria exist on the surface and body of our human body, directly or indirectly affecting the health of our human beings. [0003] Whether in nature or in our human body, bacteria are not stationary, but change the movement state by ...

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Problems solved by technology

[0007] Another purpose of the present invention is to apply the CMOS sensor to observe the movement of bacteria, so as to solve the technical problem that the frame rate of the laser confocal microscope in the prior art is too low so that real-time observation cannot be realized

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