Combined inactivated vaccine of photobacterium damselae and vibrio splendidus of pampus argenteus

A dual-inactivated vaccine and photobacteria technology, which is applied in the field of microbial vaccine preparation, can solve the problems of drug residue and drug resistance, and achieve the effects of low cost, stable output and high immune effect

Pending Publication Date: 2019-06-11
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a dual inactivated vaccine of Photobacterium mermaid and vibriosis resplendent in silver pomfret to replace antibiotics in the treatment of Photobacterium mermaid and vibriosis resplendent in order to solve drug residues and microbial resistance caused by treatment The production of medicinal properties and the reduction of silver pomfret stress stimulation and other problems make up for the deficiencies of the existing technology

Method used

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  • Combined inactivated vaccine of photobacterium damselae and vibrio splendidus of pampus argenteus
  • Combined inactivated vaccine of photobacterium damselae and vibrio splendidus of pampus argenteus
  • Combined inactivated vaccine of photobacterium damselae and vibrio splendidus of pampus argenteus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: screening photobacterium mermaid

[0014] 1. Sample collection and processing

[0015] The diseased silver pomfret was obtained from Gangwan Aquaculture Breeding Company, Xiangshan County, Ningbo City, Zhejiang Province. The abdomen of the silver pomfret was cut open with sterilized dissecting tools, the intestine was taken out, and the fat and blood on the outer wall of the intestine were washed clean with PBS buffer. Squeeze out the contents of the gastrointestinal tract slowly, then cut the gastrointestinal tract longitudinally with sterilized ophthalmic scissors, wash with PBS buffer several times, and remove the residual intestinal contents. Then use a rubber spatula to slowly scrape off the intestinal lining mucosa. The separated endolysates and mucous membranes were mixed together, put into a 10mL centrifuge tube containing PBS buffer, and mixed evenly. Use an inoculation loop to inoculate a small amount of turbid solution in the intestinal tract ...

Embodiment 2

[0043] Example 2: Preparation of inactivated vaccine by mixed strain of Photobacterium mermaid and Vibrio brilliant

[0044] 1. Preparation of inactivated vaccine

[0045] First, the Photobacterium mermaid and Vibrio resplendents screened by the present invention (purchased from the General Microbiology Center CGMCC of China Microbiological Culture Collection Management Committee, strain preservation number 1.6382) were respectively inoculated in 500mL LB medium (peptone 10g / L, yeast powder 5g / L, NaCl 10g / L, pH 7.4~7.6), 28 ℃ shaking culture for 24 hours, the concentration of the bacterial liquid detected by the hemocytometer plate reached 10 9 CFU / ml, or when the OD600 value reaches 0.6 or more, the cultivation is terminated, and the expanded culture solution of Photobacterium mermaid and Vibrio brilliantis is obtained;

[0046] Add 25ml formaldehyde solution by volume ratio 0.5% then, inactivate 48 hours at room temperature to the expanded culture solution of Vibrio splend...

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Abstract

The invention provides a combined inactivated vaccine of photobacterium damselae and vibrio splendidus of pampus argenteus. The preservation number of the photobacterium damselae is CGMCC NO.16907. The combined inactivated vaccine is simple in preparation process, stable in yield and low in cost, and a large amount of inactivated vaccines with a high immune effect can be obtained. The immune protection rate of the inactivated vaccine prepared by the method reaches 92.5%; the vaccine is a pure biological preparation, and is safe and environmentally friendly, drug residues and environmental pollution are avoided, the occurrence and prevalence of the photobacterium damselae and vibrio splendidus diseases of the pampus argenteus can be fundamentally restrained, and technical support is provided for large-scale preparation of the bigeminy inactivated vaccine for the photobacterium damselae and vibrio splendidus diseases of the pampus argenteus.

Description

technical field [0001] The invention belongs to the field of preparation of microbial vaccines, in particular to a dual inactivated vaccine of Photobacterium mermaid and Vibrio brilliantis. Background technique [0002] Silver Pomfret (Pampus argenteus) belongs to Perciformes (Perciformes), Pomfretaceae (Stromateidae), and Pomfret (Pampus). my country, Iran and other countries also account for a large proportion of fishery economy. In the past 20 years, due to overfishing and environmental pollution, wild silver pomfret resources have been shrinking, and the caught silver pomfret tends to be smaller in size. Therefore, it is particularly important to carry out artificial breeding and seedling breeding of silver pomfret. Due to the late start of domestic breeding of silver pomfret, although the bottleneck of captive breeding has been broken through, there is still a long way to go before industrialization. Especially in the breeding stage of adult fish, it is easy to be inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/106A61K39/02A61P31/04
Inventor 李渊博王亚军张曼杨阳胡佳宝余娜曹小欢匡思雯顾玮玮孙弋博
Owner NINGBO UNIV
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