Preparation method of medical micromolecular pectin

A small molecule, pectin technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of low yield and quality of pectin, achieve low impurity content, high efficiency alcohol precipitation, easy-to-remove effect

Active Publication Date: 2019-06-14
广州市莱檬生物科技有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This technical scheme process is simple, bu...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Pretreatment: crush and stir 2 tons of lemon peels to break the lemon peels into particles with a particle size of 0.2cm-2cm, add 8 tons of water, and mix evenly, maintaining the mixing temperature at 50°C;

[0041] (2) Extraction: Add the mixed solution of (1) into the medium, add 2kg of Novozymes Celluclast1.5L cellulase while stirring, add sodium bicarbonate to adjust the pH to 7.2, treat for 1 hour, and control the reaction temperature to 55°C , followed by adding nitric acid to adjust the pH to 3 for acidolysis, and stirring for 1 h;

[0042](3) Separation: use ceramic ultrafiltration membranes with a molecular weight cut-off of 30,000 and 10,000 to separate the mixed solution of (2), and collect the filtrate with a molecular weight of 10,000 to 30,000 to obtain a pectin solution with a molecular weight of 10,000 to 30,000;

[0043] (4) Purification: The liquid obtained in (3) is vacuum-concentrated, and the sugar content of the concentrated liquid is measured ...

Embodiment 2

[0047] The difference between this example and Example 1 is that (4) sodium carbonate is also added in the alcohol precipitation process, and the pH is adjusted to be 6.5-7, as described below.

[0048] (1) Pretreatment: crush and stir 2 tons of lemon peel to break the lemon peel into particles with a particle size of 0.2cm-2cm, add 8 tons of water, and mix evenly, maintaining the mixing temperature at 50°C;

[0049] (2) Extraction: Add the mixed solution of (1) into the medium, add 2kg of Novozymes Celluclast1.5L cellulase while stirring, add sodium bicarbonate to adjust the pH to 7.2, treat for 1 hour, and control the reaction temperature to 55°C , followed by adding nitric acid to adjust the pH to 3 for acidolysis, and stirring for 1 h;

[0050] (3) Separation: use ceramic ultrafiltration membranes with a molecular weight cut-off of 30,000 and 10,000 to separate the mixed solution of (2), and collect the filtrate with a molecular weight of 10,000 to 30,000 to obtain a pecti...

Embodiment 3

[0055] The difference between this example and Example 1 is that (2) 0.1 kg of subtilisin and 0.1 kg of α-amylase were added to the extraction process for enzymatic hydrolysis, as described below.

[0056] (1) Pretreatment: crush and stir 2 tons of lemon peel to break the lemon peel into particles with a particle size of 0.2cm-2cm, add 8 tons of water, and mix evenly, maintaining the mixing temperature at 50°C;

[0057] (2) Extraction: Add the mixed solution of (1) to the medium, add 2kg Novozymes Celluclast1.5L cellulase, 0.1kg subtilisin, 0.1kg α-amylase under stirring, add sodium bicarbonate to adjust the pH to 7.2, treat for 1 hour, control the reaction temperature to 55°C, then add nitric acid to adjust the pH to 3 for acidolysis, and stir for 1 hour;

[0058] (3) Separation: use ceramic ultrafiltration membranes with a molecular weight cut-off of 30,000 and 10,000 to separate the mixed solution of (2), and collect the filtrate with a molecular weight of 10,000 to 30,000 ...

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Abstract

The invention discloses a preparation method of medical micromolecular pectin. The method comprises the following steps of pretreatment, extraction, separation, purification and drying. The cellulaseand nitric acid are used for treatment; the extraction time is about 2 hours. Compared with a conventional acidolysis method, the preparation method has the advantages that the extraction efficiency can be greatly improved; the pectin molecules can be more dispersed through cellulase; the content of small molecule pectin in the solution is further improved; vacuum concentration and alcohol precipitation are used for purifying small molecule pectin; the purification purity is higher; the impurity content is small; the quality of the small molecule pectin is very good. The process design is reasonable; the obtained small molecule pectin has very good use effects in aspects of capsules of colloidal bismuth pectin capsules and the like.

Description

technical field [0001] The invention belongs to the technical field of high molecular pectin production, in particular to a preparation method of medicinal small molecular pectin. Background technique [0002] Pectin is a kind of galacturonic acid polymer that constitutes the structural components of cells, and is widely found in the cell walls of roots, stems, leaves, and fruits of various plants. Pectin has the functions of antibacterial, hemostasis, detumescence, detoxification, antidiarrheal, hypolipidemic, anti-radiation, etc. Material. However, the molecular chain of natural pectin is relatively large and cannot be absorbed by the human body. As a carrier for intravenous injection, polymer drugs need to control the molecular weight within a certain range. Studies have found that the molecular weight of pectin used as a carrier in the kidney excretion in the body should be controlled below 50,000, that is, small molecule pectin is more suitable for medical use, so a r...

Claims

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Application Information

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IPC IPC(8): C08B37/06A61K31/732
Inventor 廖劲松
Owner 广州市莱檬生物科技有限公司
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