Mesoporous silica nanosphere and its preparation method and application in drug loading

Abstract

The invention discloses a mesoporous silica nanosphere, its preparation method and its application in drug loading. The preparation method comprises: adding a silicon source solution composed of an organic silicon source and a diluent into In the mixed aqueous solution in the diluent, stir and react, filter, wash and then calcined; the particle size of the prepared sample is 40-200nm, and the specific surface area is 1050-1350m 2 / g, with a pore size of 1.8‑2.4nm; it can be applied to the loading of hydrophobic drugs to realize nanonization of drugs and enhance their water solubility and dissolution rate. The method provided by the invention can avoid the use of organic templates of traditional mesoporous silica nanospheres; the raw materials are easy to obtain, the equipment is simple, and the efficiency is high; the pore wall particles are introduced into the mesoporous structure, which is beneficial to drug diffusion and loading, and reduces the weight. Crystallization, so that the in vitro dissolution rate is almost 100%, and its bioavailability is significantly higher than that of commercially available drugs, which is of great practical significance.

Description

technical field

[0001] The invention relates to the field of nanomaterials for hydrophobic drug loading, in particular to a mesoporous silica nanosphere, a preparation method thereof, and an application in drug loading. Background technique

[0002] Oral drug delivery system is the most convenient and widely used method of drug delivery clinically. After oral administration, the drug is absorbed by the mucous membrane in the oral cavity or gastrointestinal tract, and then enters the blood circulation to reach the tissues in the body to play a therapeutic role. In the case of oral administration, the absorption process of drugs through organs such as the gastrointestinal tract is a very complex physiological process. The speed and degree of drug absorption are affected by many aspects, including the physical and chemical properties of the drug itself, the dosage form of the drug, the absorption site, and the physiological environment of the digestive tract. The main barrier...

Examples

Embodiment 1

[0056] Embodiment 1 of the present invention provides a method for preparing mesoporous silica nanospheres, the specific steps are as follows:

[0057] Measure 78mL of deionized water, 27mL of absolute ethanol, and 0.75mL of ammonia water. After mixing, add 0.3g of CTAB (cetyltrimethylammonium bromide) and ultrasonically mix to obtain solution A; measure 1.65mL of TEOS , and fully mixed with 2.175mL of absolute ethanol, wherein the volume ratio of TEOS (tetraethyl orthosilicate) to absolute ethanol is 0.758, to obtain solution B; quickly add solution B to solution A, seal, and magnetically stir React for 24 hours; then filter the obtained product with suction, wash with deionized water and absolute ethanol three times each, until neutral.

[0058] The suction-filtered product was dried at 50° C. for 12 hours, and then calcined in a muffle furnace at 550° C. for 5 hours to obtain mesoporous silica nanospheres with a diameter of 50 nm.

[0059] figure 1 and figure 2 These ar...

Embodiment 2

[0062] Embodiment 2 of the present invention provides a method for preparing mesoporous silica nanospheres, the specific steps are as follows:

[0063] Measure 78mL of deionized water, 27mL of absolute ethanol, and 0.75mL of ammonia water, mix well, add 0.3g of CTAB, and ultrasonically mix to obtain solution A. Measure 1.65mL of TEOS and fully mix with 1.25mL of absolute ethanol, wherein the volume ratio of TEOS to absolute ethanol is 1.32 to obtain solution B. Solution B was quickly added to solution A, sealed, and magnetically stirred for 24 hours. Then the obtained product was subjected to suction filtration, washed with deionized water and absolute ethanol three times, until neutral.

[0064] The suction-filtered product was dried at 50° C. for 12 hours, and then calcined in a muffle furnace at 550° C. for 5 hours to obtain mesoporous silica nanospheres with a diameter of 150 nm.

[0065] Figure 5 and Figure 6 These are the scanning electron micrographs of the mesopo...

Embodiment 3

[0067] Embodiment 3 of the present invention provides a method for preparing mesoporous silica nanospheres, the specific steps are as follows:

[0068] Measure 27mL of deionized water, 78mL of absolute ethanol, and 0.75mL of ammonia water, mix well, add 0.3g of CTAB, and ultrasonically mix to obtain solution A. Measure 1.65mL of TEOS and fully mix with 1.25mL of absolute ethanol, wherein the volume ratio of TEOS to absolute ethanol is 1.32 to obtain solution B. Solution B was quickly added to solution A, sealed, and magnetically stirred for 24 hours. Then the obtained product was subjected to suction filtration, washed with deionized water and absolute ethanol three times, until neutral.

[0069] The suction-filtered product was dried at 50° C. for 12 hours, and then calcined in a muffle furnace at 550° C. for 5 hours to obtain mesoporous silica nanospheres with a diameter of 1 μm.

[0070] Figure 7 and Figure 8 These are the scanning electron micrographs of the mesoporo...

technical field

[0001] The invention relates to the field of nanomaterials for hydrophobic drug loading, in particular to a mesoporous silica nanosphere, a preparation method thereof, and an application in drug loading. Background technique

[0002] Oral drug delivery system is the most convenient and widely used method of drug delivery clinically. After oral administration, the drug is absorbed by the mucous membrane in the oral cavity or gastrointestinal tract, and then enters the blood circulation to reach the tissues in the body to play a therapeutic role. In the case of oral administration, the absorption process of drugs through organs such as the gastrointestinal tract is a very complex physiological process. The speed and degree of drug absorption are affected by many aspects, including the physical and chemical properties of the drug itself, the dosage form of the drug, the absorption site, and the physiological environment of the digestive tract. The main barrier...

Examples

Embodiment 1

[0056] Embodiment 1 of the present invention provides a method for preparing mesoporous silica nanospheres, the specific steps are as follows:

[0057] Measure 78mL of deionized water, 27mL of absolute ethanol, and 0.75mL of ammonia water. After mixing, add 0.3g of CTAB (cetyltrimethylammonium bromide) and ultrasonically mix to obtain solution A; measure 1.65mL of TEOS , and fully mixed with 2.175mL of absolute ethanol, wherein the volume ratio of TEOS (tetraethyl orthosilicate) to absolute ethanol is 0.758, to obtain solution B; quickly add solution B to solution A, seal, and magnetically stir React for 24 hours; then filter the obtained product with suction, wash with deionized water and absolute ethanol three times each, until neutral.

[0058] The suction-filtered product was dried at 50° C. for 12 hours, and then calcined in a muffle furnace at 550° C. for 5 hours to obtain mesoporous silica nanospheres with a diameter of 50 nm.

[0059] figure 1 and figure 2 These ar...

Embodiment 2

[0062] Embodiment 2 of the present invention provides a method for preparing mesoporous silica nanospheres, the specific steps are as follows:

[0063] Measure 78mL of deionized water, 27mL of absolute ethanol, and 0.75mL of ammonia water, mix well, add 0.3g of CTAB, and ultrasonically mix to obtain solution A. Measure 1.65mL of TEOS and fully mix with 1.25mL of absolute ethanol, wherein the volume ratio of TEOS to absolute ethanol is 1.32 to obtain solution B. Solution B was quickly added to solution A, sealed, and magnetically stirred for 24 hours. Then the obtained product was subjected to suction filtration, washed with deionized water and absolute ethanol three times, until neutral.

[0064] The suction-filtered product was dried at 50° C. for 12 hours, and then calcined in a muffle furnace at 550° C. for 5 hours to obtain mesoporous silica nanospheres with a diameter of 150 nm.

[0065] Figure 5 and Figure 6 These are the scanning electron micrographs of the mesopo...

Embodiment 3

[0067] Embodiment 3 of the present invention provides a method for preparing mesoporous silica nanospheres, the specific steps are as follows:

[0068] Measure 27mL of deionized water, 78mL of absolute ethanol, and 0.75mL of ammonia water, mix well, add 0.3g of CTAB, and ultrasonically mix to obtain solution A. Measure 1.65mL of TEOS and fully mix with 1.25mL of absolute ethanol, wherein the volume ratio of TEOS to absolute ethanol is 1.32 to obtain solution B. Solution B was quickly added to solution A, sealed, and magnetically stirred for 24 hours. Then the obtained product was subjected to suction filtration, washed with deionized water and absolute ethanol three times, until neutral.

[0069] The suction-filtered product was dried at 50° C. for 12 hours, and then calcined in a muffle furnace at 550° C. for 5 hours to obtain mesoporous silica nanospheres with a diameter of 1 μm.

[0070] Figure 7 and Figure 8 These are the scanning electron micrographs of the mesoporo...

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