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Streptomycesalbogriseus and preparation method and application of antibiological inoculant of Streptomycesalbogriseus

A technology of Streptomyces albicans and biocontrol agent, which is applied in the field of preparation of Streptomyces albus and its biocontrol agent, which can solve the problems of enhanced resistance of pathogenic bacteria, environmental pollution, pesticide residues, etc., and achieve good results , no pesticide residues, easy to operate

Inactive Publication Date: 2019-06-21
BENGBU COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chemical control is widely used because of its fast effect, which causes adverse effects such as enhanced resistance of pathogenic bacteria, pesticide residues, and environmental pollution.

Method used

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  • Streptomycesalbogriseus and preparation method and application of antibiological inoculant of Streptomycesalbogriseus

Examples

Experimental program
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Effect test

Embodiment 1

[0024] A preparation method of a biocontrol fungicide for Streptomyces albus, comprising the following steps:

[0025] (1) Preparation of spore suspension: add sterile water to the slant of Streptomyces albicans Ah11601 Gao's No. 1 culture medium, scrape off the spores when Streptomyces albicans Ah11601 produces spores, and pour it into an Erlenmeyer flask Shake and mix well to obtain a spore suspension, and control the thalline concentration of the spore suspension to be 10 8 -10 9 cfu / mL;

[0026] Effective component concentration in the described Gaoshi No. 1 medium: soluble starch 20g / L, potassium nitrate 1g / L, dipotassium hydrogen phosphate 0.5g / L, sodium chloride 0.5g / L, magnesium sulfate 0.5g / L , ferrous sulfate 0.01g / L;

[0027] (2) Seed liquid culture: inoculate the spore suspension obtained in step (1) into the seed liquid medium, the inoculation amount accounts for 1% of the volume fraction of the seed liquid medium, then at 28°C, shake at a speed of 140r / min Th...

Embodiment 2

[0032] A preparation method of a biocontrol fungicide for Streptomyces albus, comprising the following steps:

[0033] (1) Preparation of spore suspension: add sterile water to the slant of Streptomyces albicans Ah11601 Gao's No. 1 culture medium, scrape off the spores when Streptomyces albicans Ah11601 produces spores, and pour it into an Erlenmeyer flask Shake and mix well to obtain a spore suspension, and control the thalline concentration of the spore suspension to be 10 8 -10 9 cfu / mL;

[0034] Effective component concentration in the described Gaoshi No. 1 medium: soluble starch 20g / L, potassium nitrate 1g / L, dipotassium hydrogen phosphate 0.5g / L, sodium chloride 0.5g / L, magnesium sulfate 0.5g / L , ferrous sulfate 0.01g / L;

[0035] (2) Seed liquid culture: inoculate the spore suspension obtained in step (1) into the seed liquid medium, the inoculation amount accounts for 2% of the volume fraction of the seed liquid medium, then at 28 ° C, shake at a speed of 200 r / min ...

Embodiment 3

[0040] A preparation method of a biocontrol fungicide for Streptomyces albus, comprising the following steps:

[0041] (1) Preparation of spore suspension: add sterile water to the slant of Streptomyces albicans Ah11601 Gao's No. 1 culture medium, scrape off the spores when Streptomyces albicans Ah11601 produces spores, and pour it into an Erlenmeyer flask Shake and mix well to obtain a spore suspension, and control the thalline concentration of the spore suspension to be 10 8 -10 9 cfu / mL;

[0042] Effective component concentration in the described Gaoshi No. 1 medium: soluble starch 20g / L, potassium nitrate 1g / L, dipotassium hydrogen phosphate 0.5g / L, sodium chloride 0.5g / L, magnesium sulfate 0.5g / L , ferrous sulfate 0.01g / L;

[0043](2) Seed liquid culture: inoculate the spore suspension obtained in step (1) into the seed liquid medium, the inoculation amount accounts for 3% of the volume fraction of the seed liquid medium, then at 28°C, shake at a speed of 220r / min The...

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Abstract

The invention provides Streptomycesalbogriseus. The Streptomycesalbogriseus Ah11601 was deposited at the General Microbiological Center of the Chinese Culture Collection and Management Committee of Microorganisms under the accession number CGMCC No. 11363. The Streptomycesalbogriseus Ah11601 antibiological inoculant is prepared from the Streptomycesalbogriseus Ah11601, and specifically comprises the following steps: adding sterile water to an inclined plane where a Streptomycesalbogriseus No. 1 culture medium is stored, scraping off spores to obtain a spore suspension, inoculating the spore suspension into a seed solution culture medium, and then carrying out shake flask culture for 50-90 h at the temperature of 28 DEG C and at the rotation speed of 140-220 r / min to obtain a seed solution;inoculating the seed liquid into a solid fermentation culture medium, carrying out koji plate fermentation culture on the solid fermentation culture medium for 5-8 days at the temperature of 28 DEG C, and then air-drying, grinding and sieving through a sieve with meshes of 120. The antibiological inoculant has a good effect of preventing and treating gray mold of peppers, drug resistance is not easily caused, pesticide residues are avoided, and the preparation method is simple.

Description

technical field [0001] The invention relates to a preparation method and application of Streptomyces albicans and its biocontrol agent. Background technique [0002] Plant fungal diseases seriously affect the growth of crops, and seriously can cause crop death, resulting in major losses such as reduced production or even no harvest. The control of plant fungal diseases usually adopts methods such as chemical control, breeding of disease-resistant varieties, and biological control. Among them, chemical control is widely used because of its fast effect, which causes adverse effects such as enhanced resistance of pathogenic bacteria, pesticide residues, and environmental pollution. Contents of the invention [0003] The first technical problem to be solved by the present invention is to provide a kind of streptomyces albicans; [0004] The second technical problem to be solved by this invention is to provide a kind of biocontrol agent of Streptomyces albicans; [0005] The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/465
Inventor 吕超田花日茂潘月敏周晶付敏
Owner BENGBU COLLEGE
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