Dry chemical reagent tablet for quantitatively detecting content of hemoglobin and preparation method of dry chemical reagent tablet
A technology for quantitative detection of hemoglobin, applied in material analysis through observation of the influence of chemical indicators, analysis through chemical reactions of materials, color/spectral characteristic measurement, etc. High equipment requirements, complex production process and other issues, to achieve good economic benefits and applications, high color intensity, high color uniformity effect
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[0059] Example 1: Preparation method of the hemoglobin dry chemical reagent tablet of the present invention
[0060] The transparent support layer is made of polyethylene terephthalate, with a thickness of 0.18 mm, a length of 20 cm, and a width of 20 cm. The surface is treated with ultraviolet irradiation, the irradiation distance is 10cm, and the irradiation time is 10 minutes.
[0061] The formula of reagent solution is as follows:
[0062] Polyvinyl alcohol 50.0g / m 2 ;
[0063] Polyethylene glycol p-isooctyl phenyl ether 20.0g / m 2 ;
[0064] Sodium carbonate 0.5mol / m 2 ;
[0065] The formula of the diffusion reagent is as follows:
[0066]
[0067] The reagent solution is uniformly coated on the polyethylene terephthalate support layer that has undergone ultraviolet treatment, and is placed in a drying box for drying. The reaction solution is sprayed on the dried reagent layer and placed in a drying box for drying.
[0068] Cut it into 12mm×12mm test strips with a cutting machine, an...
Example Embodiment
[0070] Example 2: Accuracy analysis of the method of the present invention
[0071] Test samples: blood samples of 20 medical examiners;
[0072] Comparative detection method: use a commercially available hemoglobin detection kit (methemoglobin method) single liquid reagent to detect on the Hitachi 7080 automatic biochemical analyzer, add 2.5μL sample, 250μL reagent, 37°C for 2 minutes, Measure the absorbance value at 546nm, and calculate the hemoglobin content in the sample according to the standard curve.
[0073] 20 samples were measured with the detection method of Example 1 and the comparative detection method, and the detection results are shown in Table 1.
[0074] Table 1 Analysis results
[0075]
[0076] The results show that R calculated according to the test results 2 The value is 0.9889, which is greater than 0.95, indicating that the detection result of the method of the present invention is not significantly different from the detection result of the comparison kit, and ...
Example Embodiment
[0077] Example 3: Precision analysis of the method of the present invention
[0078] Test sample: any serum sample;
[0079] The detection method of Example 1 was used to repeat the detection of the same sample to be tested 10 times, and the detection results are shown in Table 2.
[0080] Table 2 Test sample hemoglobin content (10 times) and standard deviation rate (coefficient of variation)
[0081]
[0082] The results show that the standard deviation rate is 0.96%, which is less than 10% required by the standard, indicating that the method of the present invention has high precision.
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