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Dry chemical reagent tablet for quantitatively detecting content of hemoglobin and preparation method of dry chemical reagent tablet

A technology for quantitative detection of hemoglobin, applied in material analysis through observation of the influence of chemical indicators, analysis through chemical reactions of materials, color/spectral characteristic measurement, etc. High equipment requirements, complex production process and other issues, to achieve good economic benefits and applications, high color intensity, high color uniformity effect

Inactive Publication Date: 2019-06-21
吉林省汇酉生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned dry chemical reagent sheets can be quantitatively detected, organic reagents are required for the distribution layer or diffusion layer, which requires high materials and equipment, complicated production technology, and is extremely harmful to the health of the preparation personnel and pollutes the environment.

Method used

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  • Dry chemical reagent tablet for quantitatively detecting content of hemoglobin and preparation method of dry chemical reagent tablet
  • Dry chemical reagent tablet for quantitatively detecting content of hemoglobin and preparation method of dry chemical reagent tablet
  • Dry chemical reagent tablet for quantitatively detecting content of hemoglobin and preparation method of dry chemical reagent tablet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: the preparation method of hemoglobin dry chemical reagent sheet of the present invention

[0060] The transparent support layer is made of polyethylene terephthalate, with a thickness of 0.18 mm, a length of 20 cm, and a width of 20 cm. The surface is treated by ultraviolet irradiation, the irradiation distance is 10cm, and the irradiation time is 10 minutes.

[0061] The formula of reagent solution reagent is as follows:

[0062] Polyvinyl alcohol 50.0g / m 2 ;

[0063] Polyethylene glycol p-isooctyl phenyl ether 20.0g / m 2 ;

[0064] Sodium carbonate 0.5mol / m 2 ;

[0065] The formula of the diffusion liquid reagent is as follows:

[0066]

[0067] The reagent solution is evenly coated on the UV-treated polyethylene terephthalate support layer, and put into a drying oven for drying. The reaction solution is sprayed on the dried reagent layer, and put into a drying oven for drying.

[0068] Cut it into 12mm×12mm test paper pieces with a cutting ma...

Embodiment 2

[0070] Embodiment 2: the accuracy analysis of the method of the present invention

[0071] Test samples: blood samples from 20 medical examiners;

[0072] Comparative detection method: Use commercially available hemoglobin detection kit (methemoglobin method) single liquid reagent to detect on Hitachi 7080 automatic biochemical analyzer, add 2.5 μL sample and 250 μL reagent to the automatic biochemical analyzer, react at 37°C for 2 minutes, Measure the absorbance value at 546nm, and calculate the content of hemoglobin in the sample according to the standard curve.

[0073] 20 samples were measured respectively with the detection method of Example 1 and the comparative detection method, and the detection results are shown in Table 1.

[0074] Table 1 analysis results

[0075]

[0076] The results show that the R calculated according to the test results 2 The value is 0.9889, which is greater than 0.95, showing that there is no significant difference between the detection ...

Embodiment 3

[0077] Embodiment 3: the precision analysis of the method of the present invention

[0078] Test sample: any serum sample;

[0079] Using the detection method of Example 1, the same sample to be tested was repeatedly detected 10 times, and the detection results are shown in Table 2.

[0080] Table 2 detects sample hemoglobin content (10 times) and standard deviation rate (variation coefficient)

[0081]

[0082] The result shows that the standard deviation rate is 0.96%, which is less than 10% of the standard requirement, indicating that the method of the present invention has high precision.

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Abstract

The invention belongs to the technical field of clinical in vitro diagnostic reagents and relates to a dry chemical reagent tablet for quantitatively detecting the content of hemoglobin and a preparation method of the dry chemical reagent tablet. The dry chemical reagent tablet comprises three laminations which are respectively a support layer, a reagent layer and a diffusion layer distributed sequentially; and reagents in the reagent layer include a hydrophilic colloid, a non-ionic type surfactant, and an alkaline reagent; and reagents in the diffusion layer comprise a hydrophilic high-molecular polymer, a non-ionic type surfactant and a particulate matter. The dry chemical reagent tablet of the invention has the advantages of high accuracy of detection results, high precision, high stability, long storage period, simple operation, wide application range and the like. With the dry chemical reagent tablet and the preparation method thereof adopted, professionals are not needed, and thedeficiencies of a wet chemical method can be well made up. The measurement accuracy and precision of the dry chemical reagent tablet are close to the accuracy and precision of the wet chemical determination method, so that great convenience can be provided for the field of clinical rapid detection.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro diagnostic reagents, in particular to a dry chemical reagent sheet for quantitatively detecting hemoglobin content and a preparation method thereof. Background technique [0002] Hemoglobin, also known as hemoglobin, is the main component of red blood cells. It can combine with oxygen and transport oxygen and carbon dioxide. Hemoglobin is a protein responsible for carrying oxygen in higher organisms. Hemoglobin in the human body is composed of four subunits, two α subunits and two β subunits, and the four subunits of hemoglobin can automatically assemble into α subunits in an electrolyte solution similar to the human body environment. 2 beta 2 status. Each subunit of hemoglobin is composed of a peptide chain and a heme molecule. Under physiological conditions, the peptide chain will coil and fold into a spherical shape to hold the heme molecule inside. The spherical structure formed b...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 王菁菁崔建华董理
Owner 吉林省汇酉生物技术股份有限公司
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