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Application of dracocephalum heterophyllum benth in medicines for prevention and treatment of joint damage/rheumatoid arthritis.

A technology for the rheumatoid and rheumatoid properties of Chrysanthemum heterophylla, applied in the field of medicine, can solve the problems of many side effects, etc., and achieve the effects of small side effects, abundant resources, and easy batch preparation of materials

Inactive Publication Date: 2019-06-25
江西华普康明生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet these western medicines have obvious shortcoming, and side effect is more

Method used

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  • Application of dracocephalum heterophyllum benth in medicines for prevention and treatment of joint damage/rheumatoid arthritis.
  • Application of dracocephalum heterophyllum benth in medicines for prevention and treatment of joint damage/rheumatoid arthritis.
  • Application of dracocephalum heterophyllum benth in medicines for prevention and treatment of joint damage/rheumatoid arthritis.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Preparation of the active ingredient of Qinglan heterophylla

[0036] Take the shade-dried green orchid heterophylla, put it in a pulverizer and grind it, and pass through a 60-mesh sieve. Put the pulverized product of P. heterophylla in a reflux extraction tank, add 95% ethanol, heat and reflux at 60° C. for extraction three times, filter and combine the filtrates, and concentrate under reduced pressure to obtain the extract of P. heterophyllum extract. Dissolve the extract of Indigo isophyllum extract in water, extract three times with ethyl acetate solvent, combine the extracts of ethyl acetate parts and dry under reduced pressure to obtain the extract of ethyl acetate parts.

Embodiment 2

[0037] Example 2: DBA / 1 mouse joint scoring

[0038] 18 male DBA / 1 mice were randomly divided into the following 3 groups (6 mice per group (n=6)): control group = normal mice (Control group); model group = collagen-induced arthritis mice Mice (CIA group); Experimental group = Dracocephalumheterophyllum treated CIA mice (DH group). Briefly, a certain amount of type II collagen (collagen II, CII, 2 mg / ml, Chondrex) was mixed with an equal amount of complete Freund's adjuvant (CFA, sigma) / incomplete Freund's adjuvant (IFA, sigma) . Emulsification was performed by ultrasonic emulsification to prepare CII emulsion (ie CII concentration of 1 mg / mL) and stored in a refrigerator at 4°C. All mice were administered 0.1 ml of bovine type II collagen (100 μg) emulsified in complete Freund's adjuvant intradermally at the base of the tail. Twenty-one days after the primary immunization, animals received a booster injection of bovine type II collagen (100 μg) emulsified in incomplete Fre...

Embodiment 3

[0040] Embodiment 3: DBA / 1 mouse X-ray inspection

[0041] On day 73 of the experiment, images of the ankle and foot joints of the mice were taken by small animal biopsy imaging. Then, imaging examination and analysis are performed. The degree of soft tissue swelling and bone destruction of CIA mice were observed with or without drug administration.

[0042] On the day before the end of the experiment, mice in different groups were anesthetized with chloral hydrate and scanned with X-rays and photographed, the results were as follows: image 3 shown. The results showed that in the Control group, the joint soft tissue was not swollen and the joint was not damaged. In the CIA group, the joint soft tissue was significantly swollen and the joint was damaged. In contrast, in the DH treatment group, joint soft tissue swelling and joint structural damage were significantly relieved.

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Abstract

The invention provides application of dracocephalum heterophyllum benth in medicines for prevention and treatment of joint damage / rheumatoid arthritis. The dracocephalum heterophyllum benth dracocephalum heterophyllum benth is used as a medicine for prevention and treatment of rheumatoid arthritis. The dracocephalum heterophyllum benth significantly down-regulates the levels of proinflammatory cytokines TNF-alpha, IL-6 and IL-1beta in important inflammatory sites such as spleen and paw by inhibiting joint damage and swelling; at the same time, the dracocephalum heterophyllum benth lowers the serum levels of cytokines TNF-Alpha or / and IL-6 to relieve arthritis / joint injury in mice and prevent and treat rheumatoid arthritis. The dracocephalum heterophyllum benth has significant therapeuticeffects on joint damage / rheumatoid arthritis caused by autoimmune immune response of the body and has small side effects and remarkable therapeutic effects, the material distribution is wide, resources are abundant, and preparation in batches is easy.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a traditional Chinese medicine substance capable of effectively preventing and treating joint damage / rheumatoid arthritis and its application in drugs for preventing and treating rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by synovial inflammation, cartilage and bone destruction. The incidence of RA increases with age, and women are more likely than men to develop the disease. Approximately 0.5-0.8% of people worldwide suffer from RA. Although the etiology of RA is complex, it is believed that susceptibility genes together with environmental insults, epigenetic modifications or post-translational modifications cause the activation of autoimmune responses during the development of RA. Activated dendritic cells and macrophages migrate into the synovial compartment and secrete inflammato...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61P19/02A61P37/02
Inventor 王蒲汪亚兰邹晓华王启兰党军
Owner 江西华普康明生物科技有限公司
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