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Lactobacillus plantarum and application thereof

A technology of Lactobacillus plantarum and strains, applied in the direction of application, bacteria, microorganisms, etc., to achieve the effect of ensuring safety, strong resistance to gastric juice, and good antibacterial properties

Active Publication Date: 2019-07-02
江西仁仁健康微生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are almost no relevant reports about the ability of Lactobacillus plantarum to inhibit oral pathogens (Porphyromonas gingivalis and Streptococcus mutans)

Method used

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  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof
  • Lactobacillus plantarum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening and Physiological and Biochemical Characteristics of Lactobacillus plantarum HCS03-001CGMCC No.16258 Strain

[0028] Take 5g of the feces of a healthy 8-month-old baby girl and add it to 5mL of buffered peptone solution, serially dilute it 10 times, select the appropriate dilution, and streak it onto the MRS modified medium plate. Cultivate at 37°C for 24-48 hours (put the plate in a ziplock bag); pick a single colony with typical characteristics of the target strain, larger colony, and stronger activity, and perform streak purification culture on the improved MRS medium, and repeat this process for 2-3 times. times, until the characteristics of the colonies in the streaked plate are consistent; pick more than 2 single colonies from each purified plate for smear, Gram staining, and observe under the microscope whether the color and shape of the bacteria are consistent, so as to determine the Whether the colonies in the plate are pure cultures. If th...

Embodiment 2

[0030] The Lactobacillus plantarum HCS03-001 screened in Example 2 is resistant to the adverse environment experiment of the digestive tract

[0031] 2.1 Simulated gastric juice test

[0032] Shake the bacteria liquid that has been passaged three times, take 10 mL of the bacterial suspension and centrifuge (5000×g, 10 min, 5°C) to obtain the bacterial sludge, wash it with PBS buffer for 3 times, and resuspend the obtained bacterial sludge in 10 mL of simulated gastric juice , digested at 37°C for 3 hours, and took samples to measure the number of viable bacteria.

[0033]The number of live bacteria of the test strain after activation for three generations in the third-generation culture medium is expressed by N', and the number of live bacteria measured after being digested in the simulated gastric juice medium for 3 hours is expressed by N", and the number of live bacteria of the tested strain simulated gastric juice The formula for calculating the survival rate of the test ...

Embodiment 3

[0047] Embodiment 3 antibacterial function characteristic experiment of bacterial strain

[0048] Preparation of indicator strains: Take the cryopreservation tube of the indicator strains in a water bath at 37°C to dissolve them quickly, use a micropipette to take 100 μL of the bacterial solution and add it to 10 ml of liquid medium, Escherichia coli / Staphylococcus aureus / Salmonella typhimurium / Chi Hogella / Listeria monocytogenes was cultured overnight at constant temperature at 37°C; Candida albicans was cultured at a constant temperature at 28°C for 48 hours at a constant temperature of 100rpm; Porphyromonas gingivalis was cultured overnight at a constant temperature at 37°C; Incubate overnight at constant temperature.

[0049] Prepare a solid medium, dilute the pathogenic bacteria cultivated to the logarithmic phase to a bacterial concentration of 10 5 cfu / mL, take 100 μL of the bacterial suspension and spread it evenly on the solid medium, use tweezers to place the s...

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PUM

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Abstract

The invention discloses lactobacillus plantarum and an application thereof, which belong to the technical field of microorganisms. A bacterial strain is named as lactobacillus plantarum HCS03-001. Thebacterial strain is preserved on August 14, 2018 in a "general microbiology center of China committee for culture collection of microorganisms", and the preservation number is CGMCC No.16258. The lactobacillus plantarum selected by the invention has strong capability of inhibiting oral pathogenic bacteria, and has efficacy of bowel relaxing and diarrhea resistance. The invention also discloses the application of the bacterial strain as a lyophilized powder of the additive lactobacillus plantarum with good bacteriostatic properties and especially strong capability of inhibiting the oral pathogenic bacteria in preparing food products, health-care food or a pharmaceutical composition.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Lactobacillus plantarum with strong ability of inhibiting oral pathogenic bacteria, laxative and anti-diarrhea effects and application thereof. [0002] The bacterial strain described in the present invention is named Lactobacillus plantarum HCS03-001, and the bacterial strain has been preserved in the "General Microorganism Center of China Microbial Culture Collection Management Committee" on August 14, 2018, and the preservation number is CGMCC No. 16258. Background technique [0003] When internal or external factors change and the micro-ecological system in the oral cavity is out of balance, it is easy to cause common oral diseases, such as dental caries, periodontitis, dental caries and bad breath. Dental caries and periodontal disease are oral diseases with a high incidence rate, mainly caused by oral pathogenic bacteria such as Streptococcus mutans and...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23K10/18A61K35/747A61K9/19A61P1/02A61P31/02A61P31/04A61P1/10A61P1/12C12R1/25
CPCA23V2002/00A61K9/19A61K2035/115A23K10/18A23L33/135A61P1/02A61P1/10A61P1/12A61P31/02A61P31/04C12N1/20C12N1/205C12R2001/25A23V2200/3204
Inventor 赵迪张春宇王聪耀闵祥博汤纯
Owner 江西仁仁健康微生态科技有限公司
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