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Polypeptide, CD19 single domain antibody, preparation method, nucleotide sequence and kit thereof

A nucleotide sequence and single-domain antibody technology, applied in the field of genetic engineering, can solve problems such as low binding activity, high production cost, and difficult modification

Active Publication Date: 2019-07-12
SHENZHEN PREGENE BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in recent years, a large number of new antibody drugs targeting CD19 have been continuously developed. However, the expression level of CD19 antibodies in the prior art is low, the binding activity is low, the molecular weight is large, the production cost is high, and it is not easy to modify

Method used

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  • Polypeptide, CD19 single domain antibody, preparation method, nucleotide sequence and kit thereof
  • Polypeptide, CD19 single domain antibody, preparation method, nucleotide sequence and kit thereof
  • Polypeptide, CD19 single domain antibody, preparation method, nucleotide sequence and kit thereof

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Embodiment Construction

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0037] The present invention actually proposes a polypeptide, a CD19 single domain antibody, a nucleotide sequence and a kit. The following content will introduce the polypeptide and its screening process in detail.

[0038] First, the polypeptide of the present invention has three framework regions and two variable regions:

[0039] FR1: Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly GlySer Leu Ile Leu Ser Cys Ala Ala Ser Gly Phe Thr Ph...

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Abstract

The invention discloses a polypeptide, a CD19 single domain antibody, a nucleotide sequence and a kit, wherein the polypeptide comprises four framework regions and three variable regions, and the polypeptide sequence is as follows: G1u Va1 G1n Leu Va1 G1u Ser Gly Gly Gly Leu Va 1 G1 n Pro Gly Gly Ser Leu I1e Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe I1e Ser Tyr Asp Me t Asn Trp Leu Arg Gln Thr Pro Gly Lys Gly Pro Glu Trp Val Ser Gly Ile Asn Thr Gly Gly Ser Arg Thr Tyr Tyr Ala Asp Ser Val Lys Asp Asn Thr Leu Tyr Leu Gln Met Asp Ser Leu Lys Pro Glu Asp Thr Ala Arg Tyr His Cys Ala Arg Gln Val Gly Tyr Ser Thr Leu Gly Tyr Glu Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser. According to the technical scheme, the polypeptide can specifically bind to a CD19 protein and has high binding activity.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a polypeptide, a CD19 single domain antibody, a preparation method thereof, a nucleotide sequence and a kit. Background technique [0002] CD19 is a surface protein expressed on B lymphocytes and follicular dendritic cells. It is a member of the immunoglobulin (Ig) superfamily and is a type I transmembrane glycoprotein composed of 556 amino acids. It is closely related to B cell activation, signal transduction and growth regulation. As a functional receptor molecule on the surface of B lymphocytes, CD19 constitutes a B cell double antigen binding model when the B cell antigen receptor (BCR) recognizes antigens, participates in the transport of Ca2+ in B cells, and regulates the activation and proliferation of B cells. As an important marker, CD19 is widely used in the diagnosis and prognosis of leukemia, lymphoma and immune system diseases. At the same time, since C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C12N15/13C12N15/70
CPCC07K16/2803C12N15/70C07K2317/569Y02A50/30
Inventor 李胜华包朝乐萌李莹莹余祥许莎莎
Owner SHENZHEN PREGENE BIOPHARMA CO LTD
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