Method for determining total flavonoid content in litchi seed

A determination method and technology of total flavonoids are applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., to achieve high precision, good stability, Wide linear range effect

Inactive Publication Date: 2019-07-23
GUANGXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0007] At present, there is no unified method for the determination of total flavonoids in litchi kernels. In order to better control the quality of traditi

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  • Method for determining total flavonoid content in litchi seed

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Embodiment 1

[0032] A kind of assay method of total flavonoid content in litchi core, this method is ultraviolet spectrophotometry, comprises the following steps:

[0033] (1) Preparation of reference substance solution: Accurately weigh 10.00 mg of proanthocyanidin A2 reference substance, dissolve it in methanol and constant volume to obtain a reference substance solution with a mass concentration of 1.00 mg / mL; A series of reference substance solutions of 0, 10, 25, 50, 100, 150, 200 µg / mL, set aside;

[0034] (2) Preparation of the test solution: Precisely weigh 1.00 g of lychee core powder crushed to 10 meshes, add 50 mL of methanol with a volume concentration of 50%, reflux extraction at 90°C for 30 minutes, filter while hot, and measure 1 mL To be diluted 25 times in the volumetric flask, obtain need testing solution, for subsequent use;

[0035](3) Preparation of color reaction solution: Precisely measure n-butanol, concentrated hydrochloric acid, and 10% ferric ammonium sulfate so...

Embodiment 2

[0042] A kind of assay method of total flavonoid content in litchi core, this method is ultraviolet spectrophotometry, comprises the following steps:

[0043] (1) Preparation of reference substance solution: Accurately weigh 8.00 mg of proanthocyanidin A2 reference substance, dissolve it in methanol and constant volume to obtain a reference substance solution with a mass concentration of 1.00 mg / mL; A series of reference substance solutions of 0, 10, 25, 50, 100, 150, 200 µg / mL, set aside;

[0044] (2) Preparation of the test solution: Precisely weigh 2.00 g of litchi seed powder crushed to 20 meshes, add 60 mL of methanol with a volume concentration of 50%, reflux extraction at 90°C for 50 minutes, filter while hot, and measure 1 mL To dilute 50 times in the volumetric flask, obtain need testing solution, for subsequent use;

[0045] (3) Preparation of color reaction solution: Precisely measure n-butanol, concentrated hydrochloric acid, and 10% ferric ammonium sulfate soluti...

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Abstract

The invention discloses a method for determining total flavonoid content in a litchi seed; the method is an ultraviolet spectrophotometry and comprises the following steps: (1) preparing a reference substance solution: preparing a gradient standard solution by taking procyanidine A2 as a reference substance; (2) preparing a test solution: taking litchi seed powder, adding methanol into the litchiseed powder, carrying out reflux extraction, filtering and diluting; (3) preparing a chromogenic reaction solution: uniformly mixing n-butyl alcohol, concentrated hydrochloric acid and an ammonium ferric sulfate solution according to a ratio; (4) drawing a standard curve: adding a color developing reaction solution into the reference substance solution, mixing, measuring the absorbance value, anddrawing a standard curve of absorbance value-procyanidine concentration; and (5) determining the test solution: taking the test solution, measuring the absorbance value of the test solution, and substituting into a regression equation to obtain the total flavonoid content in the litchi seed. The method in the invention is simple and rapid, the degree of precision, the reproducibility, the accuracyand the stability are good, batch measurement can be carried out, and the requirements for industrial production detection can be met.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicine components, in particular to a method for determining the content of total flavonoids in litchi kernels. Background technique [0002] Litchi core is Sapindaceae plant Litchi ( Litchi chinensis Sonn. ) dry mature seeds, also known as Li Ren, Li He, Da Li He. It is recorded in "Compendium of Materia Medica" and other ancient books that it is warm in nature, sweet in taste, slightly bitter, and returns to the liver and kidney channels. Efficacy Promoting qi and dispelling stagnation, dispelling cold and relieving pain. Modern studies have shown that lychee kernels are rich in flavonoids, volatile oils, organic acids and fatty acids and other chemical components, which have the functions of lowering blood sugar, regulating blood lipids, relieving pain, anti-inflammatory, anti-tumor, anti-oxidation, scavenging free radicals, and inhibiting platelet aggregation. 、 a...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78
CPCG01N21/33G01N21/78
Inventor 奉建芳罗伟生梁建钦冯宇冯茵怡林忆龙
Owner GUANGXI UNIV OF CHINESE MEDICINE
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