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Construction method of magnetic resonance molecular probe for targeted-marking MMP-2

A technology of MMP-2 and molecular probes, applied in the medical and health field, can solve problems such as patient suffering, limited diagnostic information, brain damage, etc., and achieve high magnetic saturation intensity and relaxation rate, and good biocompatibility

Inactive Publication Date: 2019-07-30
钟士江
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Problems solved by technology

However, this invasive examination method not only brings pain to the patient but also causes complications such as bleeding and brain damage
Traditional imaging examination methods such as CT and MRI provide limited diagnostic information, not only cannot achieve accurate grading, but it is even difficult to compare gliomas with central nervous system lesions such as demyelinating diseases, inflammatory pseudotumors, and infections. identify

Method used

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Embodiment Construction

[0017] When the present invention is specifically implemented, a method for constructing a magnetic resonance molecular probe targeting marker MMP-2, the novel molecular probe for MRI diagnosis of glioma consists of superparamagnetic iron oxide nanoparticles (superparamagnetic iron oxide nanoparticles, USPIO ) and matrix metalloproteinase-2 antibody (MMP2Ab) were synthesized by carbodiimide method.

[0018] Synthesis of USPIO-PEG superparamagnetic iron oxide nanoparticles by pyrolysis method:

[0019] 1. Weigh 15g of PEG into a 50ml three-neck round bottom flask;

[0020] 2. Under an argon atmosphere of 200ml / min, stir magnetically for 10 minutes and raise the temperature to 80°C at a constant speed;

[0021] 3. Add 0.7g of iron acetylacetonate and keep it warm for 10 minutes, then raise the temperature to 260°C within 25min at 7.2°C / min, keep it warm for 1h, stop heating, and cool naturally to about 60°C;

[0022] 4. The obtained product was ultrasonically cleaned with 60ml...

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Abstract

The invention discloses a construction method of a magnetic resonance molecular probe for targeted-marking MMP-2, wherein the novel molecular probe for MRI diagnosis of glioma is prepared from superparamagnetic iron oxide nanoparticles (USPIO) and matrix metalloproteinase-2 antibody (MMP2Ab) by using a diimide method. Compared with the prior art, the method has the advantages that the USPIO-PEG-MMP2Ab not only has higher magnetic saturation intensity and relaxation rate, but also has good biocompatibility; after intravenous administration, the USPIO-PEG-MMP2Ab can actively target glioma cellsto generate obvious negative contrast effect on magnetic resonance T2WI; and qualitative and hierarchical diagnosis of brain glioma is finished through magnetic resonance molecular imaging.

Description

technical field [0001] The invention relates to a molecular probe and a manufacturing method thereof, in particular to a molecular probe for diagnosing glioma by nuclear magnetic resonance technology (MRI) and a manufacturing method thereof, and belongs to the medical and health field. Background technique [0002] Glioma is a type of malignant disease among primary human brain tumors. Most of the patients have a survival rate of less than 5 years, and the degree of malignancy is high. The treatment of glioma is a difficult medical problem in the world. Although great progress has been made in surgery, chemotherapy and radiotherapy in recent years, the therapeutic effect of glioma has not been significantly improved. A large part of the reason is that the location of the disease is located in the central nervous system. Therefore, understanding the molecular mechanism of glioma invasion plays a key role in its early diagnosis, clear grading, and determination of treatment op...

Claims

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Application Information

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IPC IPC(8): A61K49/18
CPCA61K49/1875
Inventor 钟士江单煜恒张哲
Owner 钟士江
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