A kind of preparation method and application of pseudo sea dragon glycoprotein

A technology of glycoproteins and sea dragons, applied in the field of biochemistry, to achieve good repeatability, high product extraction rate, and ensure the integrity of the effect

Active Publication Date: 2022-08-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese Pharmacopoeia records that it has the effects of warming the kidney and strengthening yang, dispelling stagnation and reducing swelling, and has the same medicine and food. Now more and more people use it to make soup, which has a tonic effect. Studies and reports on immune system regulation

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  • A kind of preparation method and application of pseudo sea dragon glycoprotein
  • A kind of preparation method and application of pseudo sea dragon glycoprotein
  • A kind of preparation method and application of pseudo sea dragon glycoprotein

Examples

Experimental program
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Embodiment 1

[0038] A preparation method of pseudo-hyronoprotein, comprising the following steps:

[0039] ①Pretreatment: Wash the pseudo sea dragon, put it in an oven, dry at 60°C for 4 hours, then pulverize and pass through a 10-mesh sieve to obtain pseudo sea dragon coarse powder, which is weighed;

[0040] ②Degreasing treatment: Add the quasi-sea dragon coarse powder obtained in step 1 and acetone equivalent to twice its volume into a rotary reflux apparatus, and rotate and reflux for 2 hours at 60 °C, carry out degreasing, and then filter, and repeat the rotary reflux extraction of the filter residue. drying the obtained degreasing coarse powder;

[0041]③Hot water extraction: add the degreasing coarse powder obtained in step ② and distilled water into a water bath at a material-to-liquid ratio of 1:20 (g / mL), stir evenly, extract at 100°C for 2.5 hours, filter, and the obtained filtrate is used for later use, The obtained filter residue is mixed and extracted with distilled water at...

Embodiment 2

[0047] The operation of embodiment 2 is basically the same as that of embodiment 1, and the difference is:

[0048] In step (3) hot water extraction: add the degreasing coarse powder obtained in step (2) and distilled water into a water bath at a material-to-liquid ratio of 1:25 (g / mL), stir evenly, extract at 80°C for 3h, filter, and the obtained filtrate For subsequent use, the obtained filter residue is mixed and extracted with distilled water at a material-to-liquid ratio of 1:25 (g / mL) for 1 h, filtered, and the filtrates of two times were merged to obtain a pseudo-hyronoprotein mixed solution;

[0049] In step 4. concentrated alcohol precipitation: add the mixed solution obtained in step 3 into a rotary evaporator, evaporate and concentrate to 1 / 5 of the original volume at 60 °C, add 3 times the volume of ethanol to the concentrated solution, and let stand at 4 °C for 12h, Carry out alcohol precipitation, collect the precipitate after centrifuging the alcohol precipitati...

Embodiment 3

[0051] The operation of embodiment 3 is basically the same as that of embodiment 1, and the difference is:

[0052] In step (3) hot water extraction: add the degreasing coarse powder obtained in step (2) and distilled water into a water bath at a material-to-liquid ratio of 1:30 (g / mL), stir evenly, extract at 90°C for 2h, filter, and the obtained filtrate For subsequent use, the obtained filter residue is mixed and extracted with distilled water at a material-to-liquid ratio of 1:30 (g / mL) for 1 h, filtered, and the filtrates of two times are combined to obtain a pseudo-hyronoprotein mixed solution;

[0053] In the concentrated alcohol precipitation in step 4: add the mixed solution obtained in step 3 into a rotary evaporator, evaporate and concentrate to 1 / 5 of the original volume at 70°C, add 3 times the volume of ethanol to the concentrated solution, and let stand at 4°C for 16h, Carry out alcohol precipitation, collect the precipitate after centrifuging the alcohol precip...

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Abstract

The invention relates to the technical field of biochemistry, and discloses a preparation method and application of a pseudo-hyron glycoprotein. The preparation method comprises the steps of pretreatment, degreasing treatment, hot water extraction, concentrated alcohol precipitation, washing and purification, dialysis freeze-drying and the like. By comparing and optimizing the extraction process of pseudo-hyronoprotein through single-factor experiment combined with orthogonal experiment method, the optimal preparation process parameters of pseudo-hyronoprotein are obtained. , convenient operation, high product extraction rate, high purity, low preparation cost, easy popularization and application, etc. The present invention also proves that the pseudo-hyron glycoprotein has a significant effect on the immunomodulatory activity through cell experiments The application of immune regulation and the maximal development and utilization of pseudo-sea dragons provide theoretical basis and reference value.

Description

technical field [0001] The invention relates to the technical field of biochemistry, and more particularly, to a preparation method and application of a pseudo-hyronoprotein. Background technique [0002] Glycoproteins are formed by covalently binding oligosaccharides to polypeptide chains. Glycoproteins widely exist in eukaryotes, most of which have glycosylation modifications. Glycoproteins play an important role in organisms and are closely related to the occurrence of some diseases. Sugar chains have the characteristics of slight heterogeneity and non-template synthesis, which determine the complexity of sugar chain structures and the diversity of composition. Therefore, the analysis of sugar chains has not been able to make a comprehensive and complete structural identification. Glycoproteins have biological activities such as enhancing immune regulation, anti-tumor, anti-inflammatory, lowering blood sugar and lipids, anti-oxidation and anti-aging. The extraction of g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/16C07K1/14A61P37/02
CPCC07K1/36C07K1/34C07K1/30C07K1/16C07K1/145A61P37/02Y02A50/30
Inventor 黄日明杨丽红王宾郝慧丽韩玉
Owner SOUTH CHINA AGRI UNIV
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