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Multi-detection kit for breast cancer 21 gene

A detection kit and multiple detection technology, applied in the field of biomedicine, can solve the problems of heavy workload and large sample usage

Pending Publication Date: 2019-08-16
深圳塔歌生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, breast cancer 21 gene detection is through single detection, which requires a large amount of samples, and the workload of detection is also heavy. Based on this, we invented the breast cancer 21 gene multiplex detection kit

Method used

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  • Multi-detection kit for breast cancer 21 gene

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specific Embodiment

[0098] Concrete embodiment, breast cancer 21 gene multiple detection kit, this detection kit comprises 21 pairs of primers, 36 specific Taqman probes, forms 9 groups of 4 heavy primer probe mixtures, 5 * RT Buffer, reverse transcriptase, RNase Inhibitor, dNTP, 10×PCR Buffer, MgCl 2 and DNA polymerase,

[0099] The 21 pairs of primers are as follows:

[0100] ACTB-F CAGCAGATGTGGATCAGCAAG

[0101] ACTB-R GCATTTGCGGTGGACGAT

[0102] GAPDH-F ATTCCACCCATGGCAAATTC

[0103] GAPDH-R GATGGGATTTCCATTGATGACA

[0104] RPLP0-F CCATTCTATCATCAACGGGTACAA

[0105] RPLP0-R TCAGCAAGTGGGAAGGTGTAATC

[0106] TFRC-F GCCAACTGCTTTCATTTGTG

[0107] TFRC-R ACTCAGGCCCATTTCCTTTA

[0108] GUSB-F CCCACTCAGTAGCCAAGTCA

[0109] GUSB-R CACGCAGGTGGTATCAGTCT

[0110] STK15-F CATCTTCCAGGAGGACCACT

[0111] STK15-R TCCGACCTTCAATCATTTCA

[0112] CTSL2-F TGTCTCACTGAGCGAGCAGAA

[0113] CTSL2-R ACCATTGCAGCCCTGATTG

[0114] ESR1-F CGTGGTGCCCCTCTATGAC

[0115] ESR1-R GGCTAGTGGGCGCATGTAG

[0116] GRB7-F C...

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Abstract

The invention belongs to the field of biomedicine, relates to a tumor detection kit, and in particular, relates to a detection kit for risk evaluation of breast cancer recurrence. The invention relates to a multi-detection kit for a breast cancer 21 gene; according to the multi-detection kit for the breast cancer 21 gene, provided is a method for multiple detection of the breast cancer 21 gene; the method has the advantages of small used sample size, fast speed, low cost and the like, and is used for serving the majority of breast cancer patients; by early diagnosis and early treatment, required contribution is made for prolonging the life cycle of patients with breast cancer and reducing unnecessary medical expenses.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a tumor detection kit, in particular to a detection kit for breast cancer recurrence risk assessment. Background technique [0002] Breast cancer is the second most common malignancy worldwide. The latest cancer epidemiological research shows that in 2012, there were about 1.7 million new cases of breast cancer worldwide, and about 521,900 patients died of breast cancer, and the incidence rate is on the rise in recent years. The latest data show that in underdeveloped countries, breast cancer is the first cause of death from malignant tumors in women, while in developed countries, breast cancer is the second cause of death from malignant tumors in women after lung cancer. According to statistics, there are 169,000 female breast cancer cases in my country every year, which is the second most common malignant tumor in women; about 45,000 female breast cancer deaths in my country are one o...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/16
Inventor 杨雁峰
Owner 深圳塔歌生物技术有限公司
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