Epidermal growth factor liposome and preparation method thereof
A technology of epidermal growth factor and liposome, which is applied in the direction of liposome delivery, skin care preparations, skin diseases, etc., can solve the problems of moldy deterioration, poor stability of silk fibroin, drug inactivation, etc., and achieve encapsulation efficiency High, good product compatibility, easy to operate
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Embodiment 1
[0037] Embodiment 1: the preparation of epidermal growth factor liposome
[0038] According to Table 1, 2mg epidermal growth factor (hEGF), prescription quantity Soluplus, prescription quantity silk fibroin are dissolved in 5mL water; The prescription quantity liposome film-forming material is mixed with 20mL organic solvent to form an oil phase; Oil phase and water Mix with each other and ultrasonically obtain a white suspension; remove the organic solvent by rotary evaporation to form a white gel-like substance; add 75mL of water and continue to rotate to dissolve and fully hydrate the film, and make up water to 100mL to obtain the epidermal growth factor liposome.
[0039] Table 1 Epidermal growth factor liposome prescription
[0040]
[0041] Note: * indicates that the ingredients in the marked dosage are replaced by the ingredients in brackets.
Embodiment 2
[0042] Embodiment 2: particle size and surface potential of liposome
[0043] Get the epidermal growth factor liposome prepared in embodiment 1, measure its particle size and encapsulation efficiency respectively, the results are shown in Table 2. Get the epidermal growth factor liposome prepared in embodiment 1, after placing 3 months under room temperature, measure its particle size and encapsulation efficiency respectively, the results are shown in Table 2.
[0044] Particle size and encapsulation efficiency of table 2 epidermal growth factor liposome
[0045] serial number Particle size (nm) Encapsulation rate (%) Particle size after storage (nm) Encapsulation rate after storage (%) 1 147.61 56.34 150.38 49.72 2 99.49 45.58 117.32 38.38 3 159.35 20.17 166.73 17.38 4 235.67 37.42 410.63 23.42 5 125.39 17.32 133.84 9.12 6 129.47 - 173.78 - 7 162.42 15.94 183.37 8.84 8 125.39 30.28 136.74 28.71 ...
Embodiment 3
[0048]Example 3. Epidermal growth factor liposome promotes cell proliferation activity
[0049] Get the epidermal growth factor liposome that prepares in embodiment 1, utilize MTT method to detect each group liposome promotes 3T3 cell proliferation activity. Get the epidermal growth factor liposome that prepares in embodiment 1, after placing 3 months under room temperature condition, utilize MTT method to detect each group liposome promotes 3T3 cell proliferation activity.
[0050] The specific experimental process is as follows: 3T3 cells were divided into 1 × 10 6 Each well was inoculated in a 12-well culture plate, and 50 μL of liposome solution of each group was added to the cell solution in 6 wells, and a blank control group was set. Add 100 μL MTT reagent at 48 hours, incubate at 37°C for 4 hours, then add 150 μL DMSO to dissolve the formazan precipitate, measure the absorbance value of the culture solution at a wavelength of 570 nm with a microplate reader, and calcul...
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