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Cherry seedling cultivation method

A cultivation method, cherry technology, applied in the field of plant tissue culture, can solve the problems of orchard yield decline, and achieve the effects of increasing production, promoting adventitious root formation, and high absorption efficiency

Inactive Publication Date: 2019-08-23
浦江县合洪园艺研发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prunus necrotic ringspot virus infecting European sweet cherries can significantly reduce the yield of orchards by 25% to 50%, and some strains can reduce production by more than 50%. If two or more viruses co-infect European sweet cherries, the yield reduction will be even greater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A method for cultivating cherry seedlings, comprising the following steps:

[0013] 1) Disinfection of explants: Cut out plump buds that have just germinated, turned green and have not yet unfolded leaves from cherry plants, rinse them with running water for 10 minutes, then soak them in 6% washing powder for 5 minutes, disinfect them with 70% alcohol for 6 seconds, and disinfect them with 0.1% sodium hypochlorite After 15 minutes, rinse with sterile water for 4 times; peel off the outer leaf primordia of the buds in a sterile environment, and cut out the shoot apical meristem below 1.0 mm to obtain the target explant. Washing powder can reduce the surface tension of plant materials, remove the dirt slightly attached to the surface of plants, remove lipid substances, and facilitate the direct contact disinfection of alcohol and sodium hypochlorite;

[0014] 2) Induce clustered shoots: transfer the obtained explants to MS medium supplemented with 2.0mg / LZT, 0.19mg / LNAA, ...

Embodiment 2

[0019] A method for cultivating cherry seedlings, comprising the following steps:

[0020] 1) Disinfection of explants: Cut out plump buds that have just germinated, turned green and have not yet unfolded leaves from cherry plants, rinse them with running water for 12 minutes, then soak them in 6% washing powder for 5 minutes, disinfect them with 70% alcohol for 7 seconds, and disinfect them with 0.08% sodium hypochlorite After 20 minutes, rinse with sterile water for 5 times; peel off the outer leaf primordia of the buds in a sterile environment, and cut out the shoot apical meristem below 1.0 mm to obtain the target explant. Washing powder can reduce the surface tension of plant materials, remove the dirt slightly attached to the surface of plants, remove lipid substances, and facilitate the direct contact disinfection of alcohol and sodium hypochlorite;

[0021] 2) Induce clustered shoots: transfer the obtained explants to MS medium supplemented with 1.9mg / LZT, 0.21mg / LNAA,...

Embodiment 3

[0026] A method for cultivating cherry seedlings, comprising the following steps:

[0027] 1) Disinfection of explants: Cut out plump buds that have just germinated, turned green and have not yet unfolded leaves from cherry plants, rinse them with running water for 10 minutes, then soak them in 6% washing powder for 5 minutes, disinfect them with 70% alcohol for 6 seconds, and disinfect them with 0.1% sodium hypochlorite After 15 minutes, rinse with sterile water for 4 times; peel off the outer leaf primordia of the buds in a sterile environment, and cut out the shoot apical meristem below 1.0 mm to obtain the target explant. Washing powder can reduce the surface tension of plant materials, remove the dirt slightly attached to the surface of plants, remove lipid substances, and facilitate the direct contact disinfection of alcohol and sodium hypochlorite;

[0028] 2) Induce clustered shoots: transfer the obtained explants to MS medium supplemented with 2.0mg / LZT, 0.19mg / LNAA, ...

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PUM

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Abstract

The invention discloses a cherry seedling cultivation method. Newly germinating green plump buds without leaf expansion are cut from a cherry plant and serve as explants for plant tissue culture to obtain non-toxic cherry seedlings with good varieties. The cherry seedling cultivation method has the advantage that an improved 1 / 2 MS culture medium is prepared and serves as a rootless seedling rooting culture medium. All components in the 1 / 2 MS culture medium have coupling functions, the activity of polyphenol oxidase (PPO) can be effectively improved, the condensation reaction rate of catalytic IAA (indoleacetic acid) and phenolic substances is increased, production of 'IAA-phenolic acid compounds' is increased, formation of adventitious roots is promoted, grown roots are white and robust,and water and mineral substance absorption efficiency is high. Finally, the obtained cherry seedlings are virus-free, and good varieties of female parents are effectively retained.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for cultivating cherry seedlings. Background technique [0002] Due to the long-term vegetative reproduction of fruit trees, various viral diseases continue to spread and become more and more serious, resulting in poor tree growth, reduced yield, poor quality, and abnormal tree bodies, which bring huge economic losses to production. Since the 1980s, my country has introduced a large number of European sweet cherry (Prunus avium L.) varieties from abroad. Due to the blind introduction, some viral diseases that seriously affect the growth and development of cherries have also been brought in. With the large-scale promotion and cultivation of European sweet cherry in my country and the disordered seedling propagation, the spread of the virus among different plants has been accelerated, the proliferation and accumulation of the virus in plants have been promoted, and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 任倩妍
Owner 浦江县合洪园艺研发有限公司
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