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Application of wighteone in preparation of medicines for treating or preventing hand, foot and mouth disease

A technology for hand, foot and mouth disease and weiterone, which is applied in the field of medicine, can solve problems such as teratogenicity and inhibition of growth and development, and achieve various effects of drug administration

Active Publication Date: 2019-08-30
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although HFMD vaccines have been approved for marketing, their safety and effectiveness still need further clinical verification; The strategy of symptomatic treatment; and the broad-spectrum antiviral drug ribavirin has the risk of teratogenicity and inhibition of growth and development when applied to infants

Method used

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  • Application of wighteone in preparation of medicines for treating or preventing hand, foot and mouth disease
  • Application of wighteone in preparation of medicines for treating or preventing hand, foot and mouth disease
  • Application of wighteone in preparation of medicines for treating or preventing hand, foot and mouth disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The Vero cytotoxicity of embodiment 1 Weiterone

[0030] 1. Materials and methods

[0031] 1.1 Cells and culture methods

[0032] African green monkey kidney cells (Vero cells) were used as the cell model (provided by the Institute of Microbial Epidemiology, Academy of Military Medical Sciences, the same below), and DMEM medium (purchased from Jiangsu) containing 10% fetal bovine serum (purchased from Gibco Company) was used KGI Biotechnology Co., Ltd.) at 37°C, 5% CO 2 Cultivate in an incubator, and subculture when the cells grow to 90% density, and the ratio of cell subculture is 1 / 3-1 / 4.

[0033] 1.2 Reagents

[0034] MTS Cell Proliferation Quantitative Detection Kit (purchased from Promega); Weiterone (purchased from Shanghai Yuanye Biotechnology Co., Ltd., the same below).

[0035] 1.3 Instruments

[0036] Microplate reader (purchased from Perkin Elmer Company, model: EnSpire, the same below); inverted microscope (purchased from Olympus Company, model: CKX41, ...

Embodiment 2

[0047] Embodiment 2 Weiterone protects Vero cells infected with EV71 and CoxA16 virus and inhibits viral replication

[0048] 1. Materials and methods

[0049] 1.1 Cells and culture methods

[0050] African green monkey kidney cells (Vero cells) were used as the cell model, and DMEM medium containing 10% fetal bovine serum was used at 37°C and 5% CO 2 Cultivate in an incubator, and subculture when the cells grow to 90% density, and the ratio of cell subculture is 1 / 3-1 / 4.

[0051] 1.2 Virus strains

[0052] EV71 virus BJ09 / 07 strain, GenBank accession number JQ319054.1; CoxA16 virus TS10 / 08 strain, GenBank accession number JX068829, all provided by the Institute of Microbial Epidemiology, Academy of Military Medical Sciences, the same below. Determine the half cytopathic dose (TCID) of EV71 virus before use 50 ) is 10 8 / mL, CoxA16 TCID 50 for 10 8.5 / mL.

[0053] 1.3 Reagents

[0054] MTS Cell Proliferation Quantitative Detection Kit; Weiterone; Total RNA Extraction ...

Embodiment 3

[0073] Embodiment 3 The broad-spectrum research of weiterone anti-hand-foot-mouth virus

[0074] 1. Materials and methods

[0075] 1.1 Cells and culture methods

[0076] African green monkey kidney cells (Vero cells) were used as the cell model, and the cells with the number of passages between 130 and 145 were used in the experiment. Cells were cultured in DMEM medium containing 10% fetal bovine serum at 37°C, 5% CO 2 Cultivate in an incubator, and subculture when the cells grow to 90% density, and the subculture ratio is 1 / 3-1 / 4.

[0077] 1.2 Virus strains

[0078] Coxsackieviruses A groups 4, 5, 7, 9 and 10 (CoxA4, CoxA5, CoxA7, CoxA9 and CoxA10), Coxsackieviruses B groups 2 and 5 (CoxB2 and CoxB5) and echoviruses (ECHO) . Measure the TCID of each virus before use 50 10 respectively 8 、10 7.5 、10 7 、10 6 、10 8.5 、10 7.5 、10 7 and 10 7.5 / mL, the above viruses were provided by the Wuhan Institute of Virology, Chinese Academy of Sciences.

[0079] 1.3 Reagents ...

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PUM

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Abstract

The present invention provides an application of wighteone or solvates thereof or pharmaceutically acceptable salts thereof in preparation of medicines for treating or preventing hand, foot and mouthdisease. The wighteone or the pharmaceutically acceptable salt are small in toxic and side effects, can effectively inhibit cytopathic effect caused by hand, foot and mouth disease viruses, inhibit viral replication, reduce viral load, reduce or eliminate death of infected mice, prolong survival experiments, suppress inflammation, etc. The compound or the pharmaceutically acceptable salts can be further developed into the medicines for treating or preventing the hand, foot and mouth disease, have various dosage forms and administration modes, and have broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of weiterone in the preparation of medicines for treating or preventing hand, foot and mouth disease. Background technique [0002] Hand, foot and mouth disease is a childhood infectious disease caused by a virus, and it is a legal Class C infectious disease in my country. The disease mainly affects infants and young children aged 0-6, and children aged 2-3 are the most common infection. In the early stage of hand, foot and mouth disease, herpes or ulcers are caused on the extremities and mouth of the children, and most of the children are within 1-2 weeks. get well. However, the disease progresses rapidly in a small number of cases, and meningitis, aseptic encephalitis, brainstem encephalitis, encephalomyelitis, pulmonary edema, respiratory and blood circulation disorders, etc. may occur within 1-5 days of onset, and very few cases are in critical c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K36/484A61P31/14
CPCA61K31/352A61K36/484A61P31/14
Inventor 肖伟曹泽彧徐君许治良曹亮王振中
Owner JIANGSU KANION PHARMA CO LTD
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