Method for realizing batch production of cloned pigs by estrus synchronization with drugs

Abstract

The invention relates to the technical field of cloned animals, in particular to a method for realizing batch production of cloned pigs by estrus synchronization with drugs. The method comprises the following steps: S1, preparing an altrenogest solution; S2, taking a small amount of pellet feed, sucking a proper amount of the altrenogest solution by using a 10 ml injector with a needle head, uniformly spraying the altrenogest solution on the feed, meanwhile shaking while spraying to enable the feed to fully absorb the solution, and feeding the feed into a trough of surrogate sows; S3, after waiting for a few minutes, checking the trough to ensure that all the feed containing allylpregnane is eaten by each medicated surrogate sow; S4, feeding the sows with a feed containing the allylpregnane for 14 days, then stopping feeding for 7 days, and injecting the sows with 2 ml of human chorionic gonadotrophin 1000 IU on the 18th day; and S5, observing oestrus of the surrogate sows on 20th dayto 21th day. The bottleneck of estrus asynchronization and ovulation synchronization of replacement sows is solved, and a foundation is laid for large-scale production of the cloned pigs.

Description

technical field

[0001] The invention relates to the technical field of cloning animals, in particular to a method for realizing mass production of cloned pigs by using drugs to synchronize estrus. Background technique

[0002] In recent years, cloning animals has become the focus of discussions around the world, including the recent advent of somatic cell cloning monkeys that broke the bottleneck of primate cloning. During the entire process of cloning animals, a large part of scientific research institutes and universities are doing However, very few cloned pigs have actually achieved industrialization. At present, the methods of domestic production of cloned pigs are to select natural estrus multiparous sows as surrogates or select natural estrus gilts for surrogacy. The former is expensive and seriously affects Provide production tasks for surrogate sow farms, which cannot meet the demand for mass production with natural estrus gilts, because the number of natural estrus ...